Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 18 of 18 for “"GTPase activating protein(GAP)"”.
-
Molecular genetic investigation of the variability of the GTPase activating protein- (GAP-) related domain of the tuberous sclerosis-2 (TSC2) gene in TSC patients and healthy subjects
… TSC1 on chromosome 9q34.3 codes for the protein hamartin and TSC2 on chromosome 16p13.3 codes for tuberin. Tuberin shows homology to GTPase activating proteins (GAPs) and hamartin and tuberin may interact with one another to control the cell cycle, suggesting a tumor suppressor function …
-
Mechanism of action of AexU, a new type III secretion system effector from an emerging human pathogen Aeromonas hydrophila
… SSU of A. hydrophila. We identified an effector protein (designated as AexU) of the T3SS, which exhibited ADP-ribosyltransferase (ADPRT) and GTPase-activating protein (GAP) activity. AexU was successfully expressed in the HeLa cell Tet-Off system and I provided evidence that cells expressing and …
-
Identification of transcriptional mechanisms downstream of nf1 gene defeciency in malignant peripheral nerve sheath tumors
… in an inoperable location. Neurofibromin, the protein coded by the Nf1 gene, functions as a GTPase activating protein (GAP) whose mutation leads to constitutive activation of RAS and mitogen-activated protein kinase (MAPK) signaling in NF1 patientsf tumors. However, therapeutic targeting of RAS …
-
Identification of TbRP2-interacting proteins using proximity-dependent biotinylation (BioID)
The protein RP2 is a tubulin cofactor C-domain containing protein with important roles in ciliogenesis. In humans, mutations in the RP2 gene are associated with 10-15% of cases of X-linked retinitis pigmentosa; a devastating disease characterised by progressive degeneration of retinal …
-
Analysis of essential structural and functional elements in the GTP-regulated signal recognition particle cycle
… signal recognition particle (SRP)-mediated protein targeting using a combination of genetic, biochemical, and molecular sequence analysis techniques. First, I consider the SRP cycle from the perspective of molecular evolution. This analysis provides insight into the significance of …
-
An Investigation into the Intracellular Trafficking and Processing Pathways of Amyloid Precursor Protein
Amyloid precursor protein (APP) is a type-I membrane-spanning protein, believed to be central to Alzheimer’s disease (AD) pathology. It undergoes a complex intracellular trafficking and processing itinerary to produce toxic amyloid-β peptides that accumulate in extracellular plaques in the brains …
-
A NOVEL, RAS-INDEPENDENT ROLE FOR NF1 IN MICROTUBULE DYNAMICS AND DAMAGE REPAIR DICTATES SENSITIVITY TO T-DM1 IN HER2 POSITIVE BREAST CANCER.
… to the best characterized activity of NF1, its GTPase-activating protein (GAP) function that attenuates RAS signaling. NF1 encodes Neurofibromin (NF1), a large multifunctional protein consisting of 2,818 amino acids. Because of its large size and difficulty in cloning, NF1 structure has resisted …
-
Biochemical and Biophysical Studies of Novel Features of Ras-related Protein Interactions
<p>The Ras superfamily of G-proteins are of great research interest for structure-function relationships among proteins as they act as molecular switches in the regulation of various biochemical reactions in the cell. They are regulated by protein-protein interactions targeted to the highly …
-
Principles of COPII Coat Assembly
Protein secretion in eukaryotic cells begins with COPII-mediated transport from the endoplasmic reticulum (ER). The COPII coat assembles on the cytoplasmic side of the ER membrane in consecutive stages. The inner coat comprises the Sar1-Sec23/Sec24 heterotrimeric complex and is recruited through …
-
Amino acid regulation of mTORC1
… is mediated by a family of heterodimeric GTPases known as the Rags that reside on the lysosomal surface. Unique among the small GTPases, the Rags are obligate heterodimers: the highly related RagA and RagB are functionally redundant and bind to RagC or RagD, which are also very similar to …
-
Biochemical and physiological exploration of the nutrient sensing pathway upstream of mTORC1
… (mechanistic target of rapamycin complex 1) protein kinase controls growth in response to environmental cues. Aberrant mTORC1 activity is linked to numerous diseases including cancer. Understanding the regulation of mTORC1 will facilitate therapeutic developments. Amino acids promote the …
-
Rho GTPase family members in establishment of polarity in C. elegans embryos
… conserved partitioning-defective (PAR) proteins as well as the actin cytoskeleton. In Caenorhabditis elegans one-cell embryos, the PAR proteins are segregated into an anterior (PAR-3, PAR-6) and a posterior (PAR-1, PAR-2) corticaldomain. The formation of PAR polarity correlates with …
-
Characterisation of a novel Rab18 mouse model for Warburg Micro syndrome
… mutations in four members of the RAB protein network; RAB3GAP1, RAB3GAP2, RAB18 and TBC1D20, causing clinically indistinguishable phenotypes. RAB3GAP1 and RAB3GAP2 form a heterodimeric complex specifically regulating the RAB3 family of proteins in calcium mediated exocytosis of …
-
The role and function of the Ras-related protein TC21 in Neurofibromatosis type 1
… 3500 individuals worldwide. Neurofibromin, the protein mutated in NF1 disease, is a GTPase activating protein (GAP) for Ras proteins, inactivating the Ras proteins H-Ras, N-Ras, K-Ras, M-Ras, R-Ras, and TC21. Missense mutations in the GAP related domain of neurofibromin cause NF1 disease, …
-
Understanding functional mechanisms of genetic susceptibility to mycobacterial infection
… new TB-associated gene ASAP1 that encodes an Arf GTPase-activating protein (GAP). ASAP1 is known to be involved in regulation of actin and membrane remodeling. My Ph.D. included three projects. In my first project, I used RNAi and CRISPR-Cas9 technologies to study the role of ASAP1 in dendritic …
-
Spatial Control of CDC42 Activation Regulates Cell Width and Growth Zone Formation
… genes are implicated in the control of the small GTPase Cdc42. Further analyses showed two different pathways are involved: one is defined by the Cdc42 guanine-nucleotide exchange factor (GEF) Scd1 and the second by the GTPase activating protein (GAP) Rga4. Deletions of rga4 and scd1 had additive …
-
FRET-Based Conformational Sensor for the m1 Muscarinic Cholinergic Receptor
Signaling behaviors of G-protein-mediated signaling are the outcome of a regulated cycle of GTP binding and hydrolysis. Binding of GTP, which activates G proteins, is promoted by an agonist-activated receptor; and hydrolysis of bound GTP, which deactivates G proteins, is accelerated by a …
-
Studies on the Molecular Mechanisms of Tumorigenesis in Neurofibromatosis Type 1
… neurofibromin, a tumor suppressor with Ras-GAP activity that negatively regulates p21-RAS signaling. Neurofibromin deficiency typically causes chronic activation of Ras, which is thought to be a major contributor to manifestation of NF1. The cardinal features of NF1 are cafe-au-lait (CAL) …