Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"GTPase activating protein"”.
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Genetic Analysis of Grinder Formation in Caenorhabditis Elegans: Regulation by RAB-6.2 and Its GTPase Activating Protein EAT-17
… found that eat-17 encodes an ~825 amino acid Rab GTPase activating protein with a series of C-terminal coiled-coil domains. Its closest human homolog is Evi5, a putative oncogene whose function is not currently understood. Rabs are key regulators of vesicle transport, and cycle between active, GTP …
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RGA2, a putative rho-type GTPase-activating protein, is regulated by the transcription factor Tec1 during filamentous growth of Saccharomyces cerevisiae
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Biology, 1999.
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Structural and functional analysis of yeast proteins involved in ER-to-Golgi transport: Sec24p family proteins and the GTPase activating protein Gyp5p
… Ich beschreibe die Hauptmerkmale der beiden Proteine Sfb2p und Sfb3p, die ortholog zu dem COP II-Vesikelhüllprotein Sec24p sind. Diese Proteine sind höchstwahrscheinlich in die Herstellung und die Auswahl der zu verfrachtenden Ladung von COP II-Transportvesikeln eingebunden. Darüberhinaus …
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Molecular genetic investigation of the variability of the GTPase activating protein- (GAP-) related domain of the tuberous sclerosis-2 (TSC2) gene in TSC patients and healthy subjects
… TSC1 on chromosome 9q34.3 codes for the protein hamartin and TSC2 on chromosome 16p13.3 codes for tuberin. Tuberin shows homology to GTPase activating proteins (GAPs) and hamartin and tuberin may interact with one another to control the cell cycle, suggesting a tumor suppressor function …
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Mechanism of action of AexU, a new type III secretion system effector from an emerging human pathogen Aeromonas hydrophila
… SSU of A. hydrophila. We identified an effector protein (designated as AexU) of the T3SS, which exhibited ADP-ribosyltransferase (ADPRT) and GTPase-activating protein (GAP) activity. AexU was successfully expressed in the HeLa cell Tet-Off system and I provided evidence that cells expressing and …
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Identification of transcriptional mechanisms downstream of nf1 gene defeciency in malignant peripheral nerve sheath tumors
… in an inoperable location. Neurofibromin, the protein coded by the Nf1 gene, functions as a GTPase activating protein (GAP) whose mutation leads to constitutive activation of RAS and mitogen-activated protein kinase (MAPK) signaling in NF1 patientsf tumors. However, therapeutic targeting of RAS …
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The role of PAK6 in HGF-induced prostate cancer cell migration
… migration require the activity of the Rho family GTPases Rho A, Racl and Cdc42 and their effector proteins, p21-activated kinases (PAKs), which are a family of mammalian serine/threonine protein kinases. PAK6, a PAK family member, is over-expressed in prostate cancer but little is known about its …
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No-Independent Modulation of Soluble Guanylyl Cyclase (Sgc) Activity and Function
… to NO, investigators have identified a number of proteins that interact with sGC and modulate its function. For example, the interaction of sGC with ADP-ribosylation factor GTPase activating protein 1 (AGAP1) governs sGC’s intracellular distribution and therefore mediates localized production of …
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Regulation of LFA-1 on T cells by phosphoinositide signalling
… targeting all known and potential PIP3-binding proteins. Using this library, I screened the targeted genes for effects on ICAM-1-binding by a flow cytometry-based ICAM-1-binding assay, using Cas9-expressing primary mouse T cells. I identified multiple proteins regulating LFA-1-mediated adhesion …
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Discovering regulators of the amino acid sensing pathway of mTORC1
… target of rapamycin complex I (mTORC1) protein kinase functions as a master regulator of growth, and its deregulation is common in human disease, including cancer and diabetes. mTORC1 integrates multiple environmental cues to control anabolic and catabolic processes. A key input is amino …
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Uncovering The Zeb1 Interactome to Identify Novel Regulators of Metastatic Non-Small Cell Lung Cancer
… as a novel ZEB1 co-repressor and the Rab22 GTPase activating protein TBC1D2b as a ZEB1/NuRD complex target. We find that TBC1D2b suppresses E-cadherin internalization, thus hindering cancer cell invasion and metastasis. Ultimately, this project provides a novel regulatory node for ZEB1 …
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Identification of TbRP2-interacting proteins using proximity-dependent biotinylation (BioID)
The protein RP2 is a tubulin cofactor C-domain containing protein with important roles in ciliogenesis. In humans, mutations in the RP2 gene are associated with 10-15% of cases of X-linked retinitis pigmentosa; a devastating disease characterised by progressive degeneration of retinal …
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Analysis of essential structural and functional elements in the GTP-regulated signal recognition particle cycle
… signal recognition particle (SRP)-mediated protein targeting using a combination of genetic, biochemical, and molecular sequence analysis techniques. First, I consider the SRP cycle from the perspective of molecular evolution. This analysis provides insight into the significance of …
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Identifying a role for the scaffolding protein IQGAP1 in liver health and disease
… on understanding the role of the scaffolding protein IQ motif-containing GTPase-Activating Protein 1 (IQGAP1), an evolutionarily conserved scaffolding protein that is frequently up-regulated in liver disease, in the liver’s response to nutritional and cholestatic stress as well as in liver …
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An Investigation into the Intracellular Trafficking and Processing Pathways of Amyloid Precursor Protein
Amyloid precursor protein (APP) is a type-I membrane-spanning protein, believed to be central to Alzheimer’s disease (AD) pathology. It undergoes a complex intracellular trafficking and processing itinerary to produce toxic amyloid-β peptides that accumulate in extracellular plaques in the brains …
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A NOVEL, RAS-INDEPENDENT ROLE FOR NF1 IN MICROTUBULE DYNAMICS AND DAMAGE REPAIR DICTATES SENSITIVITY TO T-DM1 IN HER2 POSITIVE BREAST CANCER.
… to the best characterized activity of NF1, its GTPase-activating protein (GAP) function that attenuates RAS signaling. NF1 encodes Neurofibromin (NF1), a large multifunctional protein consisting of 2,818 amino acids. Because of its large size and difficulty in cloning, NF1 structure has resisted …
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Exploring The Impact of Decreased Ampk Pathway Activity On Brain Injury Outcome
… Akt Substrate of 160 kDa (AS160), a Rab GTPase activating protein that controls Glut4 translocation. Additionally, AMPK negatively regulates energy-consumption by inhibiting protein synthesis via the mechanistic Target of Rapamycin (mTOR) pathway.</p> <p>Given that metabolic suppression …
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Deciphering ARAP3 Functions in Hematopoiesis and Hematopoietic Stem Cells
ARAP3 is a GTPase-activating protein that inactivates Arf6 and RhoA GTPases. ARAP3 deficiency in mice causes a sprouting angiogenic defect resulting in embryonic lethality by E11. Mice with an ARAP3 R302,303A mutation (KI/KI) that prevents activation by PI3K have a similar angiogenic phenotype, …
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Biochemical and Biophysical Studies of Novel Features of Ras-related Protein Interactions
<p>The Ras superfamily of G-proteins are of great research interest for structure-function relationships among proteins as they act as molecular switches in the regulation of various biochemical reactions in the cell. They are regulated by protein-protein interactions targeted to the highly …
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Principles of COPII Coat Assembly
Protein secretion in eukaryotic cells begins with COPII-mediated transport from the endoplasmic reticulum (ER). The COPII coat assembles on the cytoplasmic side of the ER membrane in consecutive stages. The inner coat comprises the Sar1-Sec23/Sec24 heterotrimeric complex and is recruited through …
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