Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 61 for “"GTP-binding"”.
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Small gtp-binding proteins in insulin secretion
… I tested the hypothesis that Arf nucleotide binding site opener [ARNO], a guanine nucleotide exchange factor [GEF] for the small G-protein Arf6, mediates the activation of Arf6, and that ARNO/Arf6 signaling axis, in turn, controls the activation of downstream effectors. Salient features of my …
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Breaking Down Microtubule Formation: Characterizing the Biochemical and Cellular Functions of the γ-Tubulin Ring Complex
… I purified a recombinant γ-TuRC composed of GTP-binding deficient γ-tubulin in order to examine the GTP-binding dependency of the γ-TuRC's capping activity. As opposed to the γ-TuRC's nucleation activity, which is GTP-binding dependent, I found that the γ-TuRC's capping activity is …
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The generation and characterization of transgenic mice expressing modifiers of Rho GTPases
… Recent studies have suggested that Rho family GTP-binding proteins are important components of the signalling pathways that link the reception of extracellular cues to the cytoskeleton. Rho family GTP-binding proteins regulate many different aspects of the actin cytoskeleton in a wide variety …
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Molecular characterization of motility genes in Bacillus subtilis
… as flhF, was found to encode for a putative GTP-binding protein that displayed sequence similarities to components of the eukaryotic secretory apparatus. Subsequent biochemical analysis demonstrated that FlhF can bind to GTP and GDP, but not to ATP. FlhF is the first GTP-binding flagellar …
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FUNCTIONAL CHARACTERIZATION OF DENND5A/RAB6IP1 IN EARLY EMBRYONIC DEVELOPMENT
… KIAA1091 (HUGE database). Rab6 is a small GTP-binding protein that involves in intra-Golgi transport. In a yeast two hybrid screen by Bruno Goud group in 1995, they used Rab6 as bait and found several interacting cDNA clones including Rab6 interacting protein 1 or Dennd5a. Several Dennd5a …
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Genetic and cytological investigation of Nucleostemin-1 in Drosophila melanogaster
Mammalian nucleostemin (NS) is a nucleolar GTP-binding protein implicated in cell cycle progression, stem cell proliferation, and ribosome assembly. Drosophila melanogaster contains a four-member nucleostemin family (NS1-4). Nucleostemin 1 (NS1) is the closest orthologue to human NS; it shares 33% …
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Apicomplexan Class XXVII Myosin - A Computational, Biophysical and Cell Biological Characterisation of PfMyoF in Plasmodium falciparum
… both PfMyoF including two novel tail domains – a GTP binding and a WD40 domain. Bioinformatic, in vitro and cell-based assays have identified the regulatory light chains and suggest PfMyoF behaves as a plus end directed motor with a velocity similar to MYO5A. Antibodies generated against the …
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Regulation of apoptosis and desmosomes by RhoE
… epidermal function are the Rho family of small GTP-binding proteins. This thesis focuses on one member of the family, RhoE/Rnd3, and the analysis of the role it plays in the regulation of proliferation, differentiation, apoptosis and cell-cell adhesion in the epidermis. Use of RNA interference …
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Molecular characterization of the cell division gene ftsZ in Neisseria gonorrhoeae.
… known FtsZ proteins, with conservation of the GTP binding motif. In vitro transcription/translation shows the gene encodes a protein migrating at 43 kDa. Western blot analysis of gonococcal lysates and commensal Neisseria lysates detected an immunoreactive band using an anti-E. coli FtsZ …
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Interactions of the Striatal Gene Rhes with the Dopaminergic System in Rodents
… current findings favor the hypothesis that the GTP-binding protein Rhes interacts with as yet unidentified cellular proteins to buffer the transduction of synaptic dopamine signals into intracellular responses. Decreased or loss of Rhes therefore results in increased DA signal transduction.
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Mechanisms of HIV-Nef Induced Endothelial Cell Stress: Implications of HIV-Nef Protein Persistence in Aviremic HIV Patients
… host cell signaling by interacting with small GTP binding protein Rac1 which activates PAK2 to promote the release of pro-apoptotic cargo containing EV and surface expression of pro-apoptotic protein Endothelial Monocyte Activating Polypeptide II (EMAPII). Using this mechanism, Nef protein …
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Characterization of the Saccharomyces cerevisiae gene MGP1, a novel member of the ras gene superfamily that functions in the mitochondrial biogenesis
… into MGP1 that deleted the phosphate group binding region conserved in this family of guanine nucleotide binding proteins. This mutation inactivated the suppressor function of MGP1;Mgp1p co-fractionated with mitochondria in subcellular fractionation experiments. In vitro mitochondrial import …
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Analysis of essential structural and functional elements in the GTP-regulated signal recognition particle cycle
… techniques to define the role of the Srp54p GTPase in the SRP cycle. Repressing synthesis of the essential Srp54 protein produces a growth defect that correlates with an accumulation of secretory precursors. I have also analyzed the effects of 17 site-specific mutations in the G domain of …
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Amyloid β Aggregation is Coupled to Cellular Metabolism and Intramitochondrial Proteostasis
… two novel aggregation-prone proteins, Ras-like GTP-binding protein rhoA and Casein kinase I isoform alpha, form amyloid structures in a Caenorhabditis elegans model, with ageing. Using this system, we monitor the aggregation of the Amyloid β (1-42) (Aβ42) protein involved in AD, and we show that …
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The Role of Urease in the Pathogenesis of Edwardsiella ictaluri
… transporter homologue. The ureG gene encodes a GTP-binding accessory protein which is thought to function in energy-dependent urease assembly. The ureG mutant strain was found to be attenuated for mortality, persistence, and for the ability to establish infection in a competition challenge …
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Studies Of The Molecular Mechanisms Underlying Cancer Stem Cells And The Transformed Metabolic Phenotype
… aldehyde dehydrogenase 1A3 (ALDH1A3) and the GTP-binding protein/crosslinking enzyme tissue transglutaminase (tTG), two proteins suspected to have key roles in tumor initiation and the development of the malignant state, in glioma stem cells (GSCs). Additionally, I examined the contributions …
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GENOME EDITING, A NEW BIOTECNOLOGICAL APPROACH TO PRODUCE LYCOPENE-RICH PIGMENTED ORANGES
… fitoene sintasi (PSY), beta ciclasi 2 (β-LCY2) e GTP binding-protein (geni legati alla sintesi e accumulo di licopene) in polpa e albedo di arancio dolce, pompelmo e pummelo ricchi in licopene, confrontandoli con i corrispettivi mutanti non pigmentati ampliando, in questo modo, la conoscenza dei …
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Dual Regulation of Phospholipase C-beta by G betagamma
… heterotrimers by catalyzing release of GDP and binding of GTP to the G alpha subunit (Ga), releasing active Ga and G betagamma (Gbg) subunits. Activated alpha subunits of the Gq family and betagamma subunits of the Gi family stimulate phospholipase C-beta (PLC-b) isoforms to catalyze hydrolysis …
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Rab11 Regulates Lumen Formation and Morphogenesis in the Developing Pancreas
… In addition, a literature review of Rho GTPase signaling in the vasculature reinforces many of the fundamental roles of various signaling molecules that also govern pancreas development. These reports link a number of cellular processes in vivo, and presents tantalizing possibilities for …
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Hematopoietic-specific regulation of the human hex1/hex02 human rac2 genes
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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