Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"GTP hydrolysis"”.
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Biochemical and Biophysical Studies of Novel Features of Ras-related Protein Interactions
… effector binding. The kinetics of the intrinsic GTP hydrolysis reaction were compared for Cdc42 (WT and T35A) in the absence and presence of a peptide derivative of PAK21 activated kinase (PBD46). The mutation does not affect the intrinsic GTP hydrolysis rate of Cdc42, but binding with PBD46 is …
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Steps to Reconstitute in vitro a Complete Round of COPI vesicle Budding, Uncoating and Fusion
… This process was shown to be dependent on ARF1-GTP hydrolysis (Tanigawa et al., 1993), a reaction catalyzed by ARF-GTPase activating protein (ARF-GAP). Therefore myristoylated yeast ARF1p as well as its ARF-GAP (Glo3p) was produced in bacteria, purified to apparent homogeneity and in an active …
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Structural investigations of adenosylcobalamin-dependent enzyme maturation
… facilitates AdoCbl delivery by the ATR through GTP hydrolysis. In addition to the human system, there are two bacterial systems used to understand the maturation of MCM: a homologous three-component system from Methylobacterium extorquens and an analogous two-component system from Cupriavidus …
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Mechanism of Nuclear Protein Import and Export an In Vitro Approach
… cerevisiae. Using solution binding assays, GTP hydrolysis and exchange assays, as well as surface plasmon resonance we have identified various interactions between different components of the transport machinery. We propose a model for the mechanism for nuclear protein import and export. …
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Xenopus DPPA2 is a Direct Inhibitor of Microtubule Polymerization Required for Nuclear Assembly
… catastrophe. This effect is dependent on GTP hydrolysis, and we explore the possibility that Dppa2 activates the intrinsic GTPase activity of microtubule polymers.</p>
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Recombinant αβ-Tubulin and a Simple Computational Model Shed Light on the Molecular Mechanisms of Microtubule Dynamics
… mechanistic basis of important phenomena like GTP-dependent assembly and GTP hydrolysis-induced conformational change and catastrophe (the switch from growing to shrinking) remain controversial or unexplained. In Chapter 2, I discuss a study in which we used a computational model to investigate …
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MOLECULAR CHARACTERIZATION OF THE PUTATIVE NUCLEOTIDE- BINDING AND HYDROLYZING ACTIVITIES OF THE ELLIS VAN CREVELD (EVC) PROTEIN
… and leucine zipper regions. The presence of GTP analog caused the quenching of the intrinsic fluorescence of the protein, suggesting interaction between the two molecules. The fluorescence gradually recovered as the nucleotide or its hydrolysis product dissociated from the protein.</p> <p><br …
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Analysis of essential structural and functional elements in the GTP-regulated signal recognition particle cycle
… techniques to define the role of the Srp54p GTPase in the SRP cycle. Repressing synthesis of the essential Srp54 protein produces a growth defect that correlates with an accumulation of secretory precursors. I have also analyzed the effects of 17 site-specific mutations in the G domain of …
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Influence of retinal states on the development and maintenance of retinofugal projections
… G-protein signaling (RGS), which accelerate Gi/o GTP hydrolysis. Gβ5 -/- mice, which lack R7RGS activity, have malformed synapses in the outer plexiform layer (OPL) and impaired OPL transmission. Altered spontaneous retinal activity in Gβ5-/- mice at P7, P12, P14, and P28 correlates with impaired …
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Biophysical Characterization of Tryptophan Locales, Mg²+ Binding and Protein Folding in Gα Subunits
… to the subunit for guanosine 5'- triphosphate (GTP) and concomitant dissociation of the dimer.</p><p>Wild type (WT) Giα1 has three tryptophan (W) residues. Intrinsic tryptophan fluorescence of WT Giα1 and the W131F, W211F, and W258F mutants was used to investigate the localized movements of …
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Single Molecule Förster Resonance Energy Transfer and Super Resolution Force Spectroscopy Study of Elongation Factor G Evolution, Conformation, and Function
… nucleotides (nt) and tRNA translocation via the hydrolysis of one GTP molecule. The mechanism of this reaction remains largely unknown. Cryo-EM studies indicated that the switch I region of EF-G undergoes major conformational changes following GTP hydrolysis, which may drive tRNA translocation. …
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NF1 AS A REGULATOR OF CYTOSKELETON DYNAMICS AND BIOMARKER FOR DECISION-MAKING IN HER2-POSITIVE BREAST CANCER
… also associated with an increased abundance of GTP-bound tubulin, a conformational state known to cause MT stability. This raises the intriguing possibility that NF1 may directly regulate tubulin intrinsic GTP-hydrolysis, similar to its GTPase activity on Ras. Finally, we show that NF1KO cells …
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Development of in vitro iCLIP techniques to study spliceosome remodelling by RNA helicases
… after assembly. When this work was started, the GTPase Snu114 was thought to activate the helicase Brr2 to unwind the U4/U6 snRNA duplex, which ultimately leads to the formation of the spliceosome active site. To explore the role of Snu114, a complex built from Snu114 and a part of Prp8 was …
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Molecular Genetic Analysis of Tumor Cells and Developmenet of RNA Interferance Therpy in Neurofibromatosis Type1(NF1)
… protein, neurofibromin, functions as a Ras-GTPase activating protein, nothing is known about how it is normally regulated or its precise role in controlling Ras signaling pathways. Neurofibromin deficiency typically causes chronic activation of Ras, considered the major contributor to …
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NMR application and development : study of the ribosomal RNA-L11 complex
… of the 23S rRNA and jointly with RNA mediates GTPase activity of elongation and release factors. It consists of a C-terminal domain, which is the main anchor and determinant for the binding to rRNA and an N-terminal domain. The N-terminal domain plays only a limited role in RNA binding and is …
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RATE-LIMITING STEP OF CONE PHOTOTRANSDUCTION RECOVERY AND OGUCHI DISEASE MECHANISMS
… which catalyzes the exchange of GDP for GTP on the α subunit of heterotrimeric Gprotein called transducin. Activated transducin relieves inhibitory constraint on cGMP-PDE, leading to rapid hydrolysis of cGMP, closure of cGMP gated cation channels, and membrane hyperpolarization. In order …
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Modeling Pattern Formation in Biopolymer Systems induced by Reaction Kinetics and Molecular Motors
… the polymerization is actively coupled to ATP or GTP hydrolysis. A second NE state of biological relevance is caused by motor proteins. These are mobile crosslinkers that walk on the filaments whereby creating forces and reorienting or transporting the latter. The cell displays filament-related …
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Mechanics and Kinetics of Microtubules in Their Environment
… effect of the MT structural change induced by hydrolysis of GTP-tubulins on the average MT growth time before shrinkage, as a function of tubulin concentration, using a mean-first-passage-time (MFPT) method. The results are comparable with experiment in the low-medium concentration regime, …