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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 241 for “"GTP"”.
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Small gtp-binding proteins in insulin secretion
<p>Type 2 diabetes mellitus is marked by a substantial beta-cell failure which is characterized by defective insulin secretion and resistance to insulin. Understanding the molecular events leading to Glucose-stimulated insulin secretion [GSIS] might serve as therapeutic potential towards diabetes. …
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Analysis of essential structural and functional elements in the GTP-regulated signal recognition particle cycle
… techniques to define the role of the Srp54p GTPase in the SRP cycle. Repressing synthesis of the essential Srp54 protein produces a growth defect that correlates with an accumulation of secretory precursors. I have also analyzed the effects of 17 site-specific mutations in the G domain of …
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Rapid Rac GTP-ASE activation in live cells by mechanical stress is independent of Src
It is well-known that mechanical forces are crucial in regulating functions of every tissue and organ in a human body. However, it remains unclear how mechanical forces are transduced into biochemical activities and biological responses at the cellular and molecular level. Using the magnetic …
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Metabolic engineering of the pterin branch of folate synthesis by over-expression of a GTP cyclohydrolase I in peanut
… acid. To increase folate levels in peanut seed, GTP cyclohydrolase I from Arabidopsis thaliana (AtGCHI) was introduced into peanut by biolistic transformation. Plant transformation vectors were constructed using publicly available or licensable vector components to avoid intellectual property …
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Factors Affecting Translation of Messenger RNA's In Vitro: Use of a GTP Analogue to Investigate Rates of Polypeptide Chain Elongation
<p>The order of addition of amino acids to a growing protein is determined by the codon sequence of a messenger RNA molecule. This translation process was studied in vitro with a cell-free protein synthesis system derived from Escherichia coli. The rate of protein synthesis was proportional to the …
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Exploring Game Transfer Phenomena: a multimodal research approach for investigating video games' effects
… that is termed "Game Transfer Phenomena" (GTP). The Game Transfer Phenomena framework examines the influence of video games on gamers’ sensorial perception, cognitions, and behaviours directly related to video games’ structural characteristic, game content and, in-game activities. A …
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Untersuchungen zur stimulierten GTPase von EF-Tu aus Thermus thermophilus
… konnten die Unterschiede im Mechanismus der GTP-Hydrolyse und der Hydrolyse von GTP-Analoga identifiziert werden: i) Ein gamma-Phosphoamid Derivat des GTP (MAAP-GTP) wird dabei gar nicht, ein anderes Derivat (DABP-GTP), das zusätzlich eine vicinal zum gamma-Phosphat stehende Aminogruppe …
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Biopolymer Structure Analysis and Saccharification of Glycerol Thermal Processed Biomass
Glycerol thermal processing (GTP) is studied as a novel biomass pretreatment method in this research with the purposes to facilitate biopolymer fractionation and biomass saccharification. This approach is performed by treating sweet gum particles on polymer processing equipment at high temperatures …
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Studies on intracellular calcium pools in a pancreatic cell line
… In some cells, guanosine 5'-triphosphate (GTP) and arachidonic acid are known to mobilize intracellular Ca²⁺ independent of the action of IP₃ and probably play important roles in Ca²⁺ signalling. In pancreatic ductal cells the precise mechanisms of intracellular Ca²⁺ movement however are …
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Alteration of prenylation: Effect on neurite outgrowth and Rho GTPase signaling
… of prenylation precursors that alter Rho GTPase localization and loading of GTP. We show here that lovastatin decreases neurite initiation and concurrently increases GTP loading of RhoA in the cytosol and decreases GTP loading of Racl associated with the plasma membrane. We also assess how …
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Synthesis, characterization and kinetic investigations of heterophase materials prepared using group transfer polymerization
… and grafts. Group Transfer Polymerization (GTP) is a relatively new synthetic method which provides a means to obtain poly(alkyl acrylate)s and poly(alkyl methacrylate)s via a "living" mechanism over a broad temperature range. The degree of control over molecular weight and molecular weight …
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Recombinant αβ-Tubulin and a Simple Computational Model Shed Light on the Molecular Mechanisms of Microtubule Dynamics
… mechanistic basis of important phenomena like GTP-dependent assembly and GTP hydrolysis-induced conformational change and catastrophe (the switch from growing to shrinking) remain controversial or unexplained. In Chapter 2, I discuss a study in which we used a computational model to investigate …
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MOLECULAR CHARACTERIZATION OF THE PUTATIVE NUCLEOTIDE- BINDING AND HYDROLYZING ACTIVITIES OF THE ELLIS VAN CREVELD (EVC) PROTEIN
… and leucine zipper regions. The presence of GTP analog caused the quenching of the intrinsic fluorescence of the protein, suggesting interaction between the two molecules. The fluorescence gradually recovered as the nucleotide or its hydrolysis product dissociated from the protein.</p> <p><br …
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Biochemical and Biophysical Studies of Novel Features of Ras-related Protein Interactions
… effector binding. The kinetics of the intrinsic GTP hydrolysis reaction were compared for Cdc42 (WT and T35A) in the absence and presence of a peptide derivative of PAK21 activated kinase (PBD46). The mutation does not affect the intrinsic GTP hydrolysis rate of Cdc42, but binding with PBD46 is …
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Paleoenvironmental reconstruction based on palynological and palynofacies analyses of the Aptian-Albian succession in the Sergipe Basin, northeastern Brazil
… abundant group in the two wells studied. In well GTP-17-SE the second most abundant group is the spores. Well GTP-24-SE is characterized by high abundance of marine. One zone and two sub-zones and one zone of middle Albian were recognized. The absence of the three sub-zones and one zone of the …
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Molecular characterization of motility genes in Bacillus subtilis
… as flhF, was found to encode for a putative GTP-binding protein that displayed sequence similarities to components of the eukaryotic secretory apparatus. Subsequent biochemical analysis demonstrated that FlhF can bind to GTP and GDP, but not to ATP. FlhF is the first GTP-binding flagellar …
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Breaking Down Microtubule Formation: Characterizing the Biochemical and Cellular Functions of the γ-Tubulin Ring Complex
… I purified a recombinant γ-TuRC composed of GTP-binding deficient γ-tubulin in order to examine the GTP-binding dependency of the γ-TuRC's capping activity. As opposed to the γ-TuRC's nucleation activity, which is GTP-binding dependent, I found that the γ-TuRC's capping activity is …
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Purification and characterization of a smooth muscle myosin phosphatase from turkey gizzards
… of various reagents on Kbinding was determined. GTP caused a 1.4 and 4 fold increase in the Kbinding of SMP-III and SMP-IIIc, respectively. The effect of GTP on the activity of SMP-III was examined. GTP inhibited SMP-III activity towards MLC20 and HMM with an IC50 of 400 µM at pH 7.4 and 150 µM …
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Underground Wireless Mesh Communication Infrastructure Design Prediction and Optimization
… being tracked is the Ground Truth Position (GTP) and the tracking system's representation in the same coordinate system at the same time is the Tracking System Position (TSP). In an excellent tracking system the actual location, GTP, and TSP will be very close to each other. This work also …
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Evaluation and Simulation of Wireless Communication and Tracking in Underground Mining Applications
… being tracked is the Ground Truth Position (GTP) and the tracking system's representation in the same coordinate system at the same time is the Tracking System Position (TSP). In an excellent tracking system the actual location, GTP, and TSP will be very close to each other. This work also …
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