Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"GST fusion protein"”.
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Cytochrome c peroxidase : its role in nitrosative stress and purification as a GST-fusion protein
Cytochrome c Peroxidase (CCP) is a hemoprotein found in the intermembrane space of yeast mitochondria. One of its roles is to protect the cell against oxidative stress by reducing mitochondrial H 2 O 2 to H 2 O. Titration of CCP with peroxynitrite [ONOO(H)] formed a species with an absorption …
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Signal transduction by the 5-HT2A receptor and its H452Y polymorphic variant
… D (PLD) via recruitment of the small G-protein ADP-Ribosylation Factor (ARF). This thesis focusses on protein:protein interactions and signalling mechanisms of the 5-HT2AR and H452Y-5-HT2AR receptor variant. Both ARF1 and PLD1 have previously been shown to bind to the carboxy-terminal …
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Novel Cancer Therapeutics, the Generation of ROS, and Cell Survival
… unknown. The present studies examine whether the GST fusion protein, GST-MDA-7, and the adenovirus, Ad.mda-7, altered the growth and survival of the A498 and UOK121N RCC lines or radiosensitized GBM, respectively. Due to previous findings that the RCC lines, but not primary renal epithelial cells, …
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Untersuchung und Spezifizierung der molekularen Interaktion von Ubiquitin und Sumo mit dem rekombinanten a1-Glyzinrezeptor
… by sumoylation and ubiquitination of membrane proteins – in this case of the GlyR alpha 1-subunit and its ubiquitin mediated internalisation – is described. The clinical relevance of those mechanisms is wide spread and would in this case produce an inadequate receptor regulation with the …
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Recombinant expression, purification and structural characterization of aspergillus niger ring finger domain using nuclear magnetic resonance spectroscopy
e retinoblastoma binding protein 6 (RBBP6) is a 250 kDa nuclear protein which contains an N-terminal DWNN domain and a conserved RING finger domain. Previous studies determined the solution structure of the human RING finger domain using heteronuclear NMR spectroscopy. A bioinformatics analysis …
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Molecular, biochemical and functional analysis of kinesins in Leishmania mexicana
… a model to investigate flagellar kinesin motor proteins. Kinesins are a large superfamily (KIFs). More than 15 kinesin families were classified by phylogenetic analysis (Wickstead et al., 2006). Kinesins are motor proteins that convert the energy from ATP hydrolysis into mechanical work to drive …
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Engineering and characterization of single chain antibody fragments (scFvs) specific to key enzymes in polyamine biosynthesis and manipulation of polyamine pathway by constitutive expression of recombinant ODC and SDE enzymes in transgenic tobacco
… produced and affinity purified ADC, ODC-GST fusion protein and PAO-GST inclusion bodies. High antibody titers showed successful immunization. Three phage display libraries were constructed using the immunized mice repertoire. Recombinant antibodies were isolated by solid phase panning …
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Expression und Aufreinigung von intrazellulären Anteilen des Interleukin-4-Rezeptors als GST-Fusionsproteine und Messung von Protein-Protein-Wechselwirkungen
… intrazelluläre Anteile des IL-4 Rezeptors als GST-Fusionsproteine exprimiert. GST-E1, in dem das cytoplasmatische membranproximale 1/3 von IL-4Ralpha (173 AS) einschließlich des Box 1 Motivs an GST fusioniert ist, konnte nach differenzierter De- und Renaturierung und Bindung an …
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Relating the structure of the HSV-1 UL25 DNA packaging protein to its function
The herpes simplex virus type 1 (HSV-1) UL25 protein (pUL25) is a minor capsid protein that is essential for packaging the full-length viral genome into preformed precursor capsid. It is also important in virus entry and recently has been implicated in the egress of the virus from the cell (Coller …