Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 53 for “"GROWTH DEFECT"”.
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ANALYSIS OF B LYMPHOCYTES IN MOUSE MODEL LIGASE IV WITH HYPOMORPHIC MUTATION IN VDJ RECOMBINATION ASSOCIATED WITH GROWTH DEFECT
… , XRCC4 and DNA ligase IV. Rodent cells and mice defective in these components are radiation-sensitive and defective in V(D)J-recombination, showing that NHEJ also functions to rejoin DSBs introduced during lymphocyte development. We have generated a knock-in mouse model with a homozygous Lig4 …
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The electron transport chains of Neisseria meningitidis
… deficient in cytochrome c4 and c2 exhibits a growth defect under high level of oxygen. These growth defects are linked to their decreased oxygen consumption rate. The growth defect and the decreased oxygen consumption rate indicated that cytochrome c4 dominates electron transfer to cbb3 …
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Effects of aneuploidy on growth and cell cycle progression
… aneuploidy has a detrimental effect in cell growth and proliferation. The work presented here shows that most aneuploid yeast strains delay cell cycle entry by increasing the critical size for budding and by decreasing the rate of volume accumulation during the G 1 phase of the cell cycle. …
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Pleiotropic regulatory function of the lysr family transcriptional regulator cpsy during streptococcus iniae systemic infection
… mutant displays a methionine-independent growth defect in serum, which differs from the methionine-dependent defect observed for orthologous mutants of <i>S. mutans</i> and <i>S. agalactiae</i>. On the contrary, the cpsY deletion mutant can grow in excess of WT when supplemented with …
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DNA-Protein Interactions in f1 DNA Replication
… three sites within the replication enhancer. The growth defect of f1 in IHF mutants was shown to be at the level of DNA replication. This growth defect of f1 in IHF mutants is suppressed by an initiator mutation (mp1) that also suppresses the lack of the replication enhancer, indicating that the …
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The Role of Mammalian Lipid Transport Protein ORP1 During Coxiella Burnetii Infection
… While the absence of ORP1 led to a C. burnetii growth defect in MH-S cells, there was no growth defect in HeLa cells. Together, our data demonstrate that C. burnetii uses the host sterol transport protein ORP1 to promote CCV development, potentially by using ORP1 to facilitate cholesterol efflux …
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Screening for purine transporters in S. aureus
… selective purine transport conditions. Further growth characterization of the mutant revealed that the growth defect was not due to an inability to transport purines but rather to downstream effects related to the toxicity of the purine biosynthesis inhibitors. A thorough understanding of S. …
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The persistence of haploinsufficiency and its role in genome evolution
… haploinsufficient gene products are limiting for growth. In this thesis I find that having a single extra copy of haploinsufficient genes was sufficient to cause a growth defect in Saccharomyces cerevisiae.
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Outer Membrane Vesicle Production in Escherichia coli Relieves Envelope Stress and is Modulated by Changes in Peptidoglycan
… part of this work elucidated a synthetic genetic growth defect for a strain with reduced OMV production (ΔnlpA, inner membrane lipoprotein with a minor role in methionine transport) and envelope stress (ΔdegP, dual function periplasmic chaperone/ protease responsible for managing proteinaceous …
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Analytical studies of corrosion and wear resistant CrN/NbN superlattice PVD coatings.
… and pitting corrosion had occurred at selective growth defect sites, beneath which, an under-dense (porous) coating microstructure was present. These under-dense coating regions provide solution pathways through the coating to the substrate. Wherever feasible comparisons were made with CrN/NbN …
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Testing oxidative-stress hypotheses generated by systems-biology assays
… from a thyA forward mutagenesis assay and the growth defect of the yaaA deletion in DNA repair-deficient background indicated that yaaA mutants accumulated high levels of DNA damage. The growth defect of yaaA mutants was suppressed by either the addition of iron chelators or by mutations that …
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The Role Of Cardiolipin In Iron Homeostasis And Glutathione Metabolism
… cluster biogenesis. Consistent with an Fe-S defect, deletion of ISU1, one of two ISU genes that encode the mitochondrial Fe-S scaffolding protein essential for the synthesis of Fe-S clusters, led to synthetic growth defects with the crd1Δ mutant. The crd1Δ mutant exhibits decreased activities …
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Identification and functional characterization of the mitochondrial adenine nucleotide carriers of Trypanosoma brucei
… essentiality of the protein for the parasite. Growth curves of the mutant cell line proved a growth defect phenotype in various carbon sources conditions. Mitochondrial ATP production assays were performed in the mutant cell line, in presence and absence of the inducible protein, using …
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REGULATION OF DEFENSE RESPONSES MEDIATED BY BON1 AND BAP2 IN ARABIDOPSIS THALIANA
… (LOF) bon1-1 mutant allele exhibits a growth defect phenotype (dwarfed plant). bon1-1 leads to constitutive activation of SNC1 which compromises normal plant growth. BON1 has been shown to interact with two other proteins: BON1-associated protein 1 and 2 (BAP1, BAP2). The molecular …
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Insights into the consequences of chromosome gains and losses in S. cerevisiae
… leading cause of miscarriage and developmental defects, while at a cellular level, aneuploidy causes proliferative defects and is detrimental to cell fitness. However, aneuploidy is also associated with cancer, a disease of uncontrolled proliferation. Understanding the effects of aneuploidy on …
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Murine Cytomegalovirus Encodes Proteins that Regulate Viral Late Transcription
… is the leading viral cause of congenital birth defects. Murine Cytomegalovirus (MCMV) is the primary surrogate model for HCMV, and resembles the human virus with respect to virion structure, genome organization, gene expression, tissue tropism, and clinical manifestations. In my graduate …
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Regulation of the Target of Rapamycin Signaling Pathway in Saccharomyces cerevisiae
… in partial bypass of the <em>tor2-21</em> mutant growth defect at 37 ˚C.</p>
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The SEC9 gene is essential for exocytosis in yeast and encodes a dispensable amino terminal domain
… by complementation of the temperature sensitive growth defect of sec9-4 cells. The SEC9 gene is essential for viability in yeast and maps near the centromere of Chromosome XV. The cloned SEC9 gene was sequenced and contains an open reading frame with the ability to encode a 651 residue …
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Mechanistic interactions between Sulfolobus islandicus and its viruses
… are surface adhesion deficient but display no growth defect. The phenotype of this resistant cell is similar to an isolated host, Δcas6:SSV9.1, which is chronically infected leading to a superinfection exclusion hypothesis described in Chapter 3. This hypothesis proposes that downregulation of …
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In Vivo Studies of Yeast Mitochondrial Intron Splicing : Ectopic Branching and a Screen for Nuclear Encoded Splicing Factors
… of the mtDNA (13 introns) and have a respiratory growth defect. Those strains were screened by northern blot analysis for intron splicing defects. I observed the expected splicing defects in strains deleted for MSS18, CBP2 and PET54. I observed a novel splicing pattern in strains deleted for IMP1, …
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