Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"GRKs"”.
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Rhodopsin kinase structure: different nucleotide-binding states and implications for mechanism of activation of a G protein coupled receptor kinase
G protein coupled receptor (GPCR) kinases (GRKs) phosphorylate activated heptahelical receptors, leading to their uncoupling from G proteins and downregulation. The desensitization of GPCRs is critical to render cells responsive to further stimuli and if not regulated can result in many …
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Phosphorylation Bar Codes Induce Distinct Conformations and Functionalities of beta-Arrestin
… by G-protein coupled receptor kinases (GRKs) and subsequently recruit beta-arrestins. beta-arrestins are multifunctional adaptor proteins which not only desensitize G-protein signals, but also facilitate receptor internalization and mediate numerous signaling pathways on their own. As …
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Influence of Peptide Allosteric Modulators on Agonist Bias at Class B1 G Protein-Coupled Receptors
… role in cAMP signalling tested. GPCR-kinases (GRKs) are important in GPCR β-arrestin recruitment and subsequent internalisation. Attempts to identify which GRKs are responsible for phosphorylation of CLR instead identified constitutive phosphorylation and internalisation of the receptor. This …
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Ligand induzierte Phosphorylierung des Chemokin Rezeptors CCR5: strukturelle Analyse und Funktion
… auch durch G-Protein-gekoppelte Rezeptorkinasen (GRKs) phosphoryliert wird. Der Nachweis der Phosphorylierung nicht Ligand-gebundener Rezeptoren durch GRKs widerlegt das bisher gültige Konzept der heterologen Rezeptorregulation ausschließlich durch second-messenger -aktivierte Kinasen wie PKC und …
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RATE-LIMITING STEP OF CONE PHOTOTRANSDUCTION RECOVERY AND OGUCHI DISEASE MECHANISMS
… by G-protein-coupled receptor kinases (GRKs) and capped by arrestin binding. Second, activated transducin must hydrolyze bound GTP through intrinsic GTPase activity, which is accelerated by a GTPase accelerating protein (GAP) complex comprised of RGS9-1/Gβ5-L/R9AP. Mutations in human …
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ROLES OF MAST CELL G PROTEIN-COUPLED RECEPTOR KINASE 2 (GRK2) IN ALLERGY AND INFLAMMATION
… are also phosphorylated by GPCR Kinases (GRKs) to promote their desensitization. MCs express GRK2 but its role on the regulation of MRGPRX2/B2 and FcRI function has not been studied in detail. For the present study, we generated mice with MC-specific deletion of GRK2 and utilized GRK2 …
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Post-translational modifications regulate β2-Adrenoceptor signaling in cardiac myocytes
… and 346) and G protein coupled receptor kinases (GRKs) (serines 355, 356 and 364), ubiquitination (lysines 348, 372 and 375) and palmitoylation (cysteine 341). However, the physiological role of these modifications on β2AR signaling regulation in the heart remains unclear. This study provides new …
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Odorant receptors: regulation, signaling, and expression
… phosphorylation of the odorant receptor by GRKs, binding of arrestin to the receptor-ligand complex, and targeting of this moiety to clathrin-coated pits. Dynamin initiates the formation of an endocytic vesicle from the clathrin-coated pit. Data are presented that identify and localize …
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The evolution of protein kinase specificity
… case study of G-protein coupled receptor kinase (GRKs) evolution using ancestral sequence reconstructions. Third, I inferred global substrate preferences in a taxonomically broad range of species using phosphoproteome data. I then related the evolution of substrate motif sequences to that of their …
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Identifizierung der für die Agonisten-induzierte Phosphorylierung und Internalisierung relevanten Serine und Threonine in der C-terminalen Domäne des humanen Prostaglandin E2 Rezeptors, Subtyp EP4
… wurde Agonisten-abhängig, wahrscheinlich durch GRKs phosphoryliert. Nach Substitution aller in der C-terminalen Domäne lokalisierten Serine und Threonine wurde der Rezeptor nach Agonisten-Exposition nicht mehr phosphoryliert. Als potentielle Hauptphosphorylierungsstellen wurden Serine und …
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Computational and experimental investigation of G protein-coupled receptor signalling mechanisms
… between CLR, G protein Receptor Kinases (GRKs), and β-arrestins. Further investigation into CLR’s desensitisation by β-arrestins revealed interesting and unexpected results. Using Förster Resonance Energy Transfer (FRET)-based techniques, the functional expression of CLR in human primary …
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Internalisation of the type II gonadotropin-releasing hormone receptor of marmoset monkey
… of G protein-coupled receptor kinases (GRKs), dynamin-1 and β-arrestin 1 and 2 with the type II GnRH receptor. The results show that internatisation of the receptor requires GRK 2 and dynamin but does not require β-arrestin 1 and 2. Furthermore, inhibitors to both the caveolae pathway as …