Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 27 for “"GPI anchor"”.
-
Initiation and Regulation of GPI Anchor Biosynthesis in Yeast
… of the GlcNAc-PI synthase complex that initiates GPI assembly in yeast, as well as a basis for comparison to the human enzyme.
-
Complexity of the Late Stages of GPI Anchor Biosynthesis in Saccharomyces Cerevisiae
… to the third mannose of the mammalian GPI anchor. Genetic disruption of the yeast PIG-F gene (termed GPI11), demonstrated that it is essential for vegetative growth. Yeast gpi11 mutants in which the Gpi11 protein (Gpi11p) could be conditionally depleted were constructed and tested for …
-
A Family of Phosphoethanolamine Transferases That Function in GPI Anchor Assembly in Saccharomyces Cerevisiae
My results support models for branching of the GPI assembly pathway, and highlight differences between GPI synthetic enzymes of yeast and mammals that could be exploited therapeutically.
-
Characterization of the Yeast Gpi3 Protein, the Catalytic Subunit of the First Enzyme Complex in GPI Anchor Biosynthesis
I also undertook to disrupt the S. pombe gpi3 + gene and to carry out comparative studies of the fungal and human Gpi3p homologs. The S. pombe gpi3+ gene was found to be essential for cell viability in this organism. The in vivo complementation studies indicated that the S. pombe, S. cerevisiae, …
-
Aggregation & Localization of a Disease-Associated Prion Protein (PrP) Mutant
… A. Harris, Chairperson Prion protein: PrP) is a GPI-anchored sialoglycoprotein involved in the pathogenesis of infectious and inherited forms of transmissible spongiform encephalopathies: TSEs). A nine-octapeptide insertional mutation in the prion protein: PrP) causes a fatal neurodegenerative …
-
Small molecules with anti-trypanosomal and anti-leishmanial activity
… known inhibitors of glycophosphatidylinositol (GPI)-anchor biosynthesis and improving the specificity of iron chelators. GPI anchor biosynthesis is a pathway essential for survival to all three parasites. Here, a rhodanine-N-acetic acid derivative served as the starting point for the development …
-
Biosynthesis and Characterization of Early Glycosylphosphatidylinositol Anchor Intermediates in Saccharomyces Cerevisiae
… to obtain structural information on in vivo GPI anchor intermediates. To address this issue, I exploited the fact that at non-permissive temperature, the yeast dpm1--6 mutant accumulates GlcNH2-(acyl-inositol)-Pl. This precursor molecule is the earliest biosynthetic intermediate that can be …
-
Synthesis Of Functionalized Gpi Anchors And Related Glycoconjugates
… the background, significance, and synthesis of GPI anchors and GPI-anchored molecules. GPI anchors are a class of complex glycolipids with the primary purpose of attaching cell surface proteins/glycoproteins to the cell membrane. The first chapter is focused on the synthesis of functionalized …
-
Yeast Mutants That Require the GPI Biosynthetic Gene GPI1 for Growth
The isolation of t-s smp3, gaa1, and gpi2 strains using a synthetic lethality screen demonstrates that this is a valid approach to identify genes involved in GPI anchor biosynthesis. This suggests that many of the remaining S.L. mutants are also likely to harbor mutations in GPI and cell wall …
-
Development And Optimization Of The First High Throughput In Vitro Fret Assay To Characterize The Saccharomyces Cerevisiae Gpi-T
… TO CHARACTERIZE THE SACCHAROMYCES CEREVISIAE GPI TRANSAMIDASE</p> <p>By</p> <p>SANDAMALI AMARASINGHA EKANAYAKA</p> <p>December 2013</p> <p>Advisor: Dr. Tamara L. Hendrickson</p> <p>Major: Biochemistry</p> <p>Degree: Doctor of Philosophy</p> <p>The enzyme glycosylphosphatidylinositol …
-
Synthetic strategies for potential trypanocides
… on the inhibition of glycophosphoinositol (GPI) anchor synthesis by inhibiting the Zn2+-dependent enzyme, GlcNAc-PI de-N-acetylase. Trypanosomes have a variable surface glycoprotein (VSG) coat, which allows them to evade the human immune system. The GPI anchor attaches the VSG to the cell …
-
Mechanism and Evolutionary Origins of HIV-1 Virion Entrapment by Tetherin
… or pairs of glycosylphosphatidyl inositol anchors are inserted into assembling particles, while the remaining pair of membrane anchors remains embedded in the infected cell membrane. We used quantitative western blotting to determine that a few dozen tetherin dimers are used to trap each …
-
The subcellular trafficking of the prion protein: Characterisation of the function of the PrPc N-terminus
… chains and a glycosyl-phosphatidyl-inositol-(GPI)-anchor. Properly folded PrPc transits through the Golgi compartment and the secretory pathway and is attached to the outer leaflet of the plasma membrane by the GPI-anchor. The cellular function of the prion protein is still unknown, although …
-
Solution and solid-phase synthesis of potential carbohydrate vaccines for leishmaniasis and malaria
… cell rupture. A glycosylphosphatidylinositol (GPI) anchor of parasite origin was recently identified, and had the properties of a toxin. The synthesis of a modified version of the malarial GPI both in solution and on solid-phase in an automated fashion is described. The synthetic material was …
-
Molecular mechanisms of synaptic proteins
… domain-containing glycosylphosphatidylinositol (GPI) anchor 1. In the following chapters, the SAMs neurexins (NRXNs), neuroligins (NLGNs), MDGAs, and immunoglobin superfamily 21 (IgSF21) are introduced. In our studies, we characterize how the structure-function relationships of SAMs and their …
-
Molecular mechanisms of synaptic proteins
… domain-containing glycosylphosphatidylinositol (GPI) anchor 1. In the following chapters, the SAMs neurexins (NRXNs), neuroligins (NLGNs), MDGAs, and immunoglobin superfamily 21 (IgSF21) are introduced. In our studies, we characterize how the structure-function relationships of SAMs and their …
-
Characterization of a Novel GPI-anchored Protein, a Component of Sphingomyelin-enriched Microdomains at the Golgi Complex
… and subsequent characterizations show that it is GPI-anchored and therefore a luminal protein. It is a Golgi resident protein and has a Brefeldin A-sensitive Golgi localization. Therefore, this protein was annotated GREG for Golgi-resident GPI-anchored protein. Mapping the Golgi-targeting signal …
-
Chemical tools to probe carbohydrate recognition and glycoconjugate biosynthesis
… activities involved in the decoration of GPI anchor in Trypanosoma brucei. Further application of this methodology allowed detection and characterisation of mannosyltransferase activities responsible for biosynthesis of N-glycans in Euglena gracilis, along with detection of …
-
IDENTIFICATION OF THE ROLE OF [METHYL]GLUCURONIC ACID ON ARABINOGALACTAN POLYSACCHARIDES IN ARABIDOPSIS THALIANA
… localised mainly at the plasma membrane via a GPI-anchor. They can also be soluble in the apoplast. Their low abundance, chemical similarity and high functional redundancy have hindered their study. My strategy to overcome these difficulties was to study knock-out Arabidopsis thaliana plants of …
-
Syncytiotrophoblast formation is influenced by genes involved in lipid biology
… fusion. Tmed2 and Pgap2 are involved in GPI anchor biosynthesis, and the KO cells have specific phenotypes regarding SynT-I differentiation. The effects of seeding density indicate that the abundance of progenitor cells has a profound impact on trophoblast lineage differentiation, …
Page 1 of 2