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Showing 1 to 20 of 96 for “"GPI"”.
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Biological studies of GPI-anchoring in Trypanosoma cruzi
… membrane by\r\nglycosylphosphatidylinositol (GPI) anchors and play roles in host cell attachment and\r\ninvasion, and in parasite differentiation and replication. In other organisms, a\r\ntransamidase, GPI8, is involved in this anchoring process. This study investigated the\r\neffects of …
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Synthesis Of Functionalized Gpi Anchors And Related Glycoconjugates
… the background, significance, and synthesis of GPI anchors and GPI-anchored molecules. GPI anchors are a class of complex glycolipids with the primary purpose of attaching cell surface proteins/glycoproteins to the cell membrane. The first chapter is focused on the synthesis of functionalized …
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Initiation and Regulation of GPI Anchor Biosynthesis in Yeast
… of the GlcNAc-PI synthase complex that initiates GPI assembly in yeast, as well as a basis for comparison to the human enzyme.
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Expression and behaviour of GPI-anchored surface proteins in trypanosomes
… number of different proteins, some of which are GPI-anchored. This thesis explores the expression and behaviour of trypanosome surface proteins and is split into two parts. The first part focuses on the link between the expression of VSG and the control of cellular growth in the African …
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Biochemical And Structural Characterization Of The Core Subunits Of Gpi Transamidase
… CHARACTERIZATION OF THE CORE SUBUNITS OF GPI TRANSAMIDASE</p> <p>by</p> <p>DILANI G GAMAGE</p> <p>May 2015</p> <p>Advisor: Prof. Tamara L. Hendrickson</p> <p>Major: Chemistry (Biochemistry)</p> <p>Degree: Doctor of Philosophy</p> <p>Glycosylphosphatidylinositol transamidase (GPI-T) is a …
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Yeast Mutants That Require the GPI Biosynthetic Gene GPI1 for Growth
The isolation of t-s smp3, gaa1, and gpi2 strains using a synthetic lethality screen demonstrates that this is a valid approach to identify genes involved in GPI anchor biosynthesis. This suggests that many of the remaining S.L. mutants are also likely to harbor mutations in GPI and cell wall …
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Gpi-Anchored Proteins In Ciliated Protozoa: From Ca++ Signaling To Mitochondrial Extrusion
… of plasma and ciliary membranes enriched in GPI-anchored proteins known as immobilization antigens or i-antigens. Antibodies against these proteins trigger a number of behavioral changes in ciliates including discharge of cortical secretory granules and arrest of cell movement (hence the term …
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Essential GPI Mannosyltransferases in Saccharomyces Cerevisiae and the Pathogenic Fungus Candida Albicans
… transfers a fourth mannose to a trimannosyl-GPI precursor in vivo. I determined that CaSMP3 is also an essential gene, as I was unable to recover homozygous null smp3 mutants from C. albicans, an obligate diploid. I created a conditional strain in which expression of CaSMP3 was controlled by …
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Complexity of the Late Stages of GPI Anchor Biosynthesis in Saccharomyces Cerevisiae
… to the third mannose of the mammalian GPI anchor. Genetic disruption of the yeast PIG-F gene (termed GPI11), demonstrated that it is essential for vegetative growth. Yeast gpi11 mutants in which the Gpi11 protein (Gpi11p) could be conditionally depleted were constructed and tested for …
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A Family of Phosphoethanolamine Transferases That Function in GPI Anchor Assembly in Saccharomyces Cerevisiae
My results support models for branching of the GPI assembly pathway, and highlight differences between GPI synthetic enzymes of yeast and mammals that could be exploited therapeutically.
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Characterization of a Novel GPI-anchored Protein, a Component of Sphingomyelin-enriched Microdomains at the Golgi Complex
… and subsequent characterizations show that it is GPI-anchored and therefore a luminal protein. It is a Golgi resident protein and has a Brefeldin A-sensitive Golgi localization. Therefore, this protein was annotated GREG for Golgi-resident GPI-anchored protein. Mapping the Golgi-targeting signal …
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Characterization of the Yeast Gpi3 Protein, the Catalytic Subunit of the First Enzyme Complex in GPI Anchor Biosynthesis
I also undertook to disrupt the S. pombe gpi3 + gene and to carry out comparative studies of the fungal and human Gpi3p homologs. The S. pombe gpi3+ gene was found to be essential for cell viability in this organism. The in vivo complementation studies indicated that the S. pombe, S. cerevisiae, …
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Development And Optimization Of The First High Throughput In Vitro Fret Assay To Characterize The Saccharomyces Cerevisiae Gpi-T
… TO CHARACTERIZE THE SACCHAROMYCES CEREVISIAE GPI TRANSAMIDASE</p> <p>By</p> <p>SANDAMALI AMARASINGHA EKANAYAKA</p> <p>December 2013</p> <p>Advisor: Dr. Tamara L. Hendrickson</p> <p>Major: Biochemistry</p> <p>Degree: Doctor of Philosophy</p> <p>The enzyme glycosylphosphatidylinositol …
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Aggregation & Localization of a Disease-Associated Prion Protein (PrP) Mutant
… A. Harris, Chairperson Prion protein: PrP) is a GPI-anchored sialoglycoprotein involved in the pathogenesis of infectious and inherited forms of transmissible spongiform encephalopathies: TSEs). A nine-octapeptide insertional mutation in the prion protein: PrP) causes a fatal neurodegenerative …
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The Role of Glucose -6 -Phosphate Isomerase in Implantation in the Ferret (Mustela Putorius)
I concluded that GPI is the 60 kilodalton implantation protein. GPI is produced and secreted by ferret CL on days 6--9 of pregnancy in ferrets and is required for embryo implantation. Further study is needed to determine whether prolactin, which is also required for implantation, affects GPI …
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Characterisation of the pre-invasion glycophosphatidylinositol-anchored surface proteins of Plasmodium falciparum merozoites
… erythrocytes. Glycophosphatidylinositol (GPI) -anchored proteins cover most of the exterior surface of the merozoite prior to invasion, while other GPI-anchored proteins are released onto the merozoite surface through apical organelle secretions. These proteins are involved in interactions …
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Biosynthesis and Characterization of Early Glycosylphosphatidylinositol Anchor Intermediates in Saccharomyces Cerevisiae
… to obtain structural information on in vivo GPI anchor intermediates. To address this issue, I exploited the fact that at non-permissive temperature, the yeast dpm1--6 mutant accumulates GlcNH2-(acyl-inositol)-Pl. This precursor molecule is the earliest biosynthetic intermediate that can be …
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Isolation and molecular genetic analysis of conditionally lethal yeast mutants in glycosylphosphatidylinositol anchoring
Glycosylphosphatidylinositol (GPI) anchors are a recently described class of glycolipids attached through covalent linkage to protein. To explore the functions of GPIs, I investigated the effects of GPI anchoring deficiencies in the unicellular eukaryote Saccharomyces cerevisiae.
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Studies of Edge Fluctuations of Negative Triangularity Plasma on TCV using a New Gas-Puff Imaging Diagnostic
… δ plasmas on TCV using Gas-Puff Imaging (GPI). GPI measures the spatially-resolved edge fluctuations by imaging atomic spectral-line emission from a local neutral gas puff. In collaboration with the TCV team at EPFL, a new GPI system was installed at TCV and has been operational since …
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The glypican Dally binds to Lipophorin particles and increases Hedgehog signaling efficiency
… and Hedgehog. They also bind a wide variety of gpi-linked proteins. Whether any of these proteins affect morphogen signaling is unknown. Here, I show that the gpi-linked heparan sulfate proteoglycan Dally is released from cell membranes and binds to lipoprotein particles both with and without …
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