Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"GP5"”.
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Purification of the major envelope protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions
… indicates that the major envelope protein, GP5, is the primary target for a subunit vaccine. In native virions GP5 primarily exists as a disulfide linked complex with the membrane protein, M, which also possesses immunogenic properties. Recent studies report that the GP5/M complex is a more …
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Reverse Genetics-based Approaches to Attenuate Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
… approach to rapidly attenuate PRRSV. The GP5 envelope genes of 7 genetically divergent PRRSV strains and the GP5-M genes of 6 different PRRSV strains were molecularly bred. DS722 with shuffled GP5 genes and DS5M3 with shuffled GP5-M genes, were successfully rescued in vitro and shown to be …
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Molecular characterization of the major envelope protein of porcine reproductive and respiratory syndrome virus (PRRSV) and evaluation of its use for a diagnostic assay, vaccine development, and the examination of quasispecies evolution
… (ORF5) gene encoding the major envelope protein (GP5) of acute PRRSV isolates was characterized. Sequence and phylogenetic analyses revealed that seven of the acute PRRS virus (PRRSV) isolates were related to other N. American PRRSV isolates while one isolate, 98-37120-2, was very closely related …
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Intranasal Immunization of Pigs with Porcine Reproductive and Respiratory Syndrome Virus like Particles Plus 2’, 3’ –cGamp Vaccigrade™ Adjuvant Exacerbates Viremia After Virus Challenge
… contain PRRSV nucleocapsid (N), glycoprotein 5 (GP5), membrane (M), and the envelope (E) proteins. There were 3 groups of pigs vaccinated with PBS for a control group, a VLPs group, and VLPs group with the 2’3’-cGAMP adjuvant. Vaccination occurred once initially and again as a boost after 2 …
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Human Papillomavirus DNA extraction and genotype analysis by multiplex real time polymerase chain reaction from formalin fixed paraffin wax-embedded cervical carcinoma specimens
… a touchdown PCR technique with L1 consensus gene GP5+/GP6+ primers. HrHPV genotypes were detected using a four colour multiplex hrHPV genotyping kit. Samples showing positive results in overlapping probe filter detection spectra were subjected to DNA Sanger sequencing for final confirmation of …
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Interaction of the porcine reproductive and respiratory syndrome virus with the macrophage-specific lectin sialoadhesin
… sialic acids were found on the GP3, GP4 and GP5 envelope glycoproteins, only the M/GP5 glycoprotein complex of PRRSV was identified as a ligand for pSn. The interaction was found to be dependent on the sialic acid binding capacity of pSn and on the presence of sialic acids on the GP5 protein. …
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Infección por Virus de Papiloma Humano (HPV) en carcinomas epidermoides de laringe, hipofaringe y orofaringe
… ADN de alto riesgo del HPV se realizó mediante GP5 +/6+-PCR e hibridación in situ (ISH). En los casos positivos se realizó una RT-PCR de ARNm de E6. Resultados: en OPSCC treinta casos (12%) fueron positivos para la inmunotinción p16, de los cuales ocho (3,2% de la serie total) fueron positivos …
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Effects of variation in N-linked glycosylation site on antibody mediated neutralization in PRRSV
… neutralizing immune responses. Analysis of GP5 sequences of a subset of field strains showed that the N34 glycosylation site can shift to N33 or N35. Comparing these variations to the virus neutralization titers, we observed that the closer the glycosylation site (N35) was to the epitope, …
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Development of Virus-like particles (VLPs) Based Vaccines Against Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Porcine Epidemic Diarrhea Virus (PEDV)
… from PRRSV structural proteins GP3 and GP5 respectively. Candidate GP3-4 was able to stimulate a significant viral neutralizing response in mouse sera against two PRRSV strains, one being heterologous, demonstrating its potential of cross-protection against PRRSV. The second animal study …
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Antigenic profiling of the porcine reproductive and respiratory syndrome virus
… set of envelope proteins (GP2, E, GP3, GP4, GP5 and M) upon infection with LV was investigated. Twenty-one antigenic regions were identified that are capable of inducing antibodies in pigs. Peptide-purified antibodies against four antigenic regions turned out to reduce in vitro virus …
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Development of subunit vaccines against porcine reproductive and respiratory syndrome virus (PRRSV)
… consisting of the ectodomains of viral M and GP5 proteins was expressed and purified. The protein product showed a single band on a silver-stained gel and contained an endotoxin level of less than 10 EU/mg protein. In addition, the purified protein showed expected bioactivities. It was …