Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 120 for “"GMP"”.
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GMP „Quo vadis?“ Entwicklung eines spezifischen Bewertungstools „GMP-DAKS-Σ“ für die qualitative und quantitative Analyse von GMP-Systemen in onkologisch herstellenden Apotheken
… aim of the present dissertation is to verify how GMP guidelines contribute to the strategic positioning of pharmacies producing carcinogenic, mutagenic and reprotoxic substances (CMR) and by which measurable indicators these pharmacies are characterised before and after implementation of a GMP …
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Cyclic-di-GMP Signaling in the Borrelia Spirochetes
… of the second messenger nucleotide, cyclic-di-GMP (c-di-GMP). Rrp1 is a response regulatory-diguanylate cyclase, and its regulatory capability is likely mediated via production of c-di-GMP, as it lacks a DNA-binding domain. One known class of c-di-GMP effector/binding proteins are those that …
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Molecular Genetic Analysis of Cyclic GMP Signaling in Higher Plants
… mediated by the small signaling molecule cyclic GMP (cGMP). In addition, sucrose is an inhibitor of phytochrome-regulated gene expression, but the manner in which it achieves these effects remains undefined. It was also found that the signaling pathways of sucrose and phytochrome synergistically …
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Manufacturing GMP-grade mRNA vaccine-associated Enzymes: Bioprocess Development and Economics
Africa’s near-total dependence on imported GMP-grade recombinant enzymes for mRNA vaccine production represents a critical vulnerability in continental health security, exposed most acutely during the SARS-CoV-2 pandemic. This thesis provides a framework for estimating the financial viability of …
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Characterization of cyclic-di-GMP signaling with the Lyme spirochete, Borrelia burgdorferi
… studies have suggested cyclic diguanylate (c-di-GMP), a ubiquitous secondary messenger, is a key modulator of B. burgdorferi adaptive responses and may play a significant role in cycle progression. In this study, we examined the impact of the sole diguanylate cyclase (Rrp1), c-di-GMP binding …
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Roles of Cyclic GMP-AMP Synthase in Immune Defense Against Retroviruses and Autoimmunity
… known to trigger robust innate immunity. Cyclic GMP-AMP synthase (cGAS) is the sensor of cytosolic DNA and activation of cGAS initiates cytokine production. Here we show cGAS plays essential roles in immune defense against retroviruses as well as in autoimmune diseases caused by self-DNA. HIV …
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Characterization of C-Gmp Dependent Kinases Within the Circadian Molecular Clockworks: Structural, Phylogenetic, and Functional Analyses
cGKI and cGKII are paralogous serine/threonine protein kinases. In an effort to determine the mechanism by which the circadian molecular clockworks differentially regulates the activity of these kinases a comparative biology approach was employed. This work included phylogenetic and 3-dimensional …
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Characterization of cyclic di-GMP binding by the sole Borrelia burgdorferi and Borrelia hermsii PilZ domain-containing proteins
… in various environmental conditions. C-di-GMP has been demonstrated to be important in bacterial adaptation. Borrelia deletion mutant phenotypes have shown that c-di-GMP regulates motility, infectivity, and enzootic cycle progression. As the only known receptors encoded by Borrelia, PlzA …
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Cyclic di-GMP signalling and the regulation of virulence, biofilm formation and epiphytic fitness in the phytopathogen <i>Xanthomonas campestris</i>
… role is played by the second messenger cyclic di-GMP. C-di-GMP is essential for successful infection of the host not only by <i>Xcc</i> but also by many other pathogens. Roles for c-di-GMP include the regulation of motility, production of virulence determinants and the switch from a motile to a …
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Régulation du c-di-GMP et rôle de ce messager secondaire dans la formation de pili de type IV chez Clostridium difficile.
Malgré la découverte du c-di-GMP en 1987, ce n’est que durant la dernière décennie que l’importance de ce messager secondaire dans la régulation des phénotypes bactériens a été exposée. Synthétisé par des diguanylate cyclases (DGC) et dégradé par des phosphodiestérases spécifiques (PDE), le …
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Immunohistochemistry for studying cyclic nucleotides in the central nervous system, with particular reference to guanosine 3',5'-monophosphate
… histochemically localizing cyclic AMP and cyclic GMP in the C.N.S., and determining the cellular sites where biochemical changes in tissue levels occur. Initial experiments revealed that the inmunoglobulin fraction of non -immune sera bound non -specifically to C.N.S. tissue, via a weak charge …
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Effects of Cisplatin Analog Size on the Reaction with DNA Bases
… were synthesized and their effects on AMP and GMP were studied using NMR spectroscopy. The experiments were conducted to examine the effects of bulk on 5’-GMP and 5’-AMP. The results suggest that bulk slows down the reaction with AMP more than with that of GMP. The order of reactivity is …
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Effect of Leaving Ligands of Platinum(II) Diamine Complexes on DNA and Protein Residues
… ligands on the relative reactivity between 5'-GMP (guanosine 5' phosphate), a key DNA target, and N-Acetyl - L-Methionine (N-AcMet), a key protein target. We have used NMR spectroscopy to monitor reactions with N-AcMet and 5'-GMP added to a platinum complex to see which products are formed …
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Production of hepatocytes from human pluripotent stem cells for cell-based therapy
… not compatible with Good Manufacturing Practice (GMP) as they rely on culture media which are poorly defined and contain reagents of animal origin. Secondly, HLCs derived in vitro lack the full spectrum of adult hepatocyte functionalities and expressed foetal markers indicating that they are not …
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Caracterização reológica e estrutural de géis ácidos das proteínas α-lactalbumina, β-lactoglobulina e glicomacropeptídeo
… e, com exceção da mistura com maior proporção de GMP, formaram géis autossustentáveis. Géis compostos com menor proporção de β-Lg apresentaram menor módulo elástico. Maior proporção de α-La aumentou o tempo de gelificação da mistura e consequentemente diminuiu o pH de gelificação, enquanto maior …
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Derivados da guanina e a modulação da captação astrocitária de glutamato
… de glutamato foi observada quando usamos GUO, GMP e GTP. Quando utilizamos os derivados de Guanina simultaneamente não observamos efeito aditivo. Nós também investigamos se interconversões entre as purinas derivadas da guanina têm influência no efeito observado. O efeito do GTP foi …
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Function, localization and regulation of the Pseudomonas aeruginosa diguanylate cyclase response regulator WspR
… on surfaces. Active WspR produces c-di-GMP, which in turn stimulates biofilm formation. Previous work demonstrated that when phosphorylated in response to growth on surfaces, WspR has a tendency to form oligomers that are visible in cells as subcellular clusters. In this study, I sought …
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Essays on the Size Distribution of Cities
… referred to as gross metropolitan product, GMP). We build a model of the spatial economy that includes production and confirm the following empirical facts about the GMP counterpart of the city-size distribution. First, both Zipf's and Gibrat's law hold for the distribution of GMP as well. …
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