Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"GINS"”.
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Human GINS, a conserved DNA replication factor and candidate cancer marker
The GINS complex (a heterotetramer of Sld5, Psf1, Psf2 and Psf3) is a highly conserved DNA replication factor required for the initiation and elongation of DNA replication. GINS is believed to associate with Cdc45 and MCM proteins on replicating DNA. The interaction between GINS and MCM is also …
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Functional dissection of the S. cerevisiae helicase activating factor Cdc45
… of the helicase activating proteins Cdc4S and GINS. The resulting Cdc45/Mcm2-7/GINS (CMG) complex unwinds double-stranded DNA at the replication fork. Although it is clear that CMG complex possesses robust helicase activity in vitro that is dependent on GINS and Cdc45, it is unclear how the CMG …
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A Proteomic and Genomic Investigation Into Replication Fork Dynamics in Saccharomyces Cerevisiae
… between the replication fork-coupled GINS complex and other components of the replication machinery. The scope of this technique is extended by applying it to cells that have been synchronized within the cell cycle — revealing the cell cycle dependent interactions of the GINS complex. …
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DNA damage responses in the context of the cell division cycle
… with DNA damage responses by regulation of the GINS complex, and checkpoint activation by the prototypical checkpoint protein Rad9. Here, I show that mitotic cells treated with DNA break-inducing agents activate a ‘primary’ DDR, including ATM and DNA-PK-dependent H2AX phosphorylation and …
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Single-molecule studies of the mechanism of eukaryotic helicase activation
… and only once per cell cycle. To ensure that origins only initiate once per cell cycle, the events of DNA replication initiation are temporally separated to different phases of the cell cycle. This regulation separates two key events that center the replicative DNA helicase Mcm2-7: helicase …
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Elucidation of Snm1B Function
… of Snm1B. From this screen I identified PSF2 (GINS2), a member of the tetrameric protein complex GINS, to bind Snm1B. The GINS complex is required for replication initiation and elongation. Of interest, the knockdown of PSF2 sensitizes cells to ICL inducing agents. I therefore tested the …
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Mechanism of Mcm10 function during DNA replication
… Mcm2-7. Mcm2-7 complexes are loaded around origins of DNA replication during G1 phase. As cells transition from G1 to S phase, the Mcm2-7 helicases are activated resulting in the recruitment of DNA polymerases and accessory proteins to begin DNA synthesis. Activation of the Mcm2-7 replicative …
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Treslin and its role in the assembly of the replicative DNA helicase
… activity, protein co-activators of Cdc45 and GINS have to be recruited to assemble a replicative DNA helicase called the CMG (Cdc45-Mcm2–7-GINS) complex. The active CMG subsequently unwinds the parental DNA duplex to provide single-stranded DNA templates for replicative DNA polymerases. The …
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The Roles of the Mcm2-7 Tails in Replication Initiation
… In G1 phase, preparation for replication begins when two copies of the replicative helicase, the Mcm2-7 complex, are loaded onto DNA. However, these helicases are loaded in an inactive state, and are not activated until the following S phase. As cells enter S phase, the action of two …
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Molecular Profiling: Gene Expression Reveals Discrete Phases of Lens Induction and Development in Xenopus Laevis
… during DNA replication as a member of the GINS heterotetramer (Kubota et al., 2003), a ""sliding clamp"" involved in DNA replication. In situ hybridization revealed that Psf2 is expressed during embryonic development in a tissue-specific manner, including the optic cup and the lens. …
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Investigation of Cdc45 function during helicase activation
… loading of Mcm2-7 helicase complexes at origins of replication. These loaded helicases need to be activated during DNA replication initiation to unwind the DNA double helix and provide a ssDNA template for replicative DNA polymerases. Eukaryotic cells segregate helicase loading and …
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An analysis of an alternative marketing system for cotton involving delayed ginning through various storage techniques
For technical reasons, cotton gins normally operate at constant rates, and vary hours of operation to accommodate available volume. In this sense, time becomes an input and, in the case of cotton gins, a cost reducing input as the proportions of fixed to variable costs is extremely high. …
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Functional and structural investigations of human replisomes assembled from purified proteins
… the eukaryotic replisome is the CMG (CDC45-MCM-GINS) helicase that unwinds the parental DNA duplex to generate single-stranded DNA for initiation of DNA synthesis by Pol α -primase and the replication of both strands by the replicative polymerases Pol ε and Pol δ. Replisomes contain multiple …
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Essays on banking
… tackles the determination of bank interest mar-gins using a simple production-based model of risk-neutral banks which face (i) loan default risk, (ii) interest rate risk, (iii) capital regulation, and (iv) deposit insurance. The optimal bank interest margin is shown to be increasing with the …
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Analyse multicritère de la durabilité de la production et mise en marché du gin par les microdistilleries québécoises
… microdistilleries émergent rapidement et que les gins québécois acquièrent progressivement des parts de marché importantes, une analyse de la durabilité de ce processus est nécessaire afin de limiter les impacts potentiels de cette filière au niveau environnemental, économique, social et culturel …
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In vitro reconstitution of the ubiquitylation and disassembly of the eukaryotic replisome
… the genome is copied only once. The Cdc45-MCM-GINS (CMG) DNA helicase which is the core of the eukaryotic replication complex, has been shown to be extensively regulated by post translational modifications, during its assembly. Therefore, it is not inconceivable that the process to unload the …
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The Role of the Protein MTBP in DNA Replication
… DNA replication occurs at replication origins, licensed by loaded double hexamers of Mcm2-7 proteins. The action of two kinases, DDK and S-CDK, triggers the loading onto Mcm2-7 of Cdc45 and the GINS complex to form the replicative CMG helicase.<br/><br/>In yeast, the proteins Sld2 and Sld3 …
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Charakterisierung des humanen Proteins MCM8 und seiner Interaktion mit CDC45
… zur Ausübung seiner Funktion. CDC45 und der GINS-Proteinkomplex gelten als essentielle Cofaktoren der Helikase MCM2-7, welche zusammen als CMG-Komplex den DNA-Doppelstrang entwinden. Wenn MCM8 als Helikase in die DNA-Replikation der eukaryotischen Zelle involviert ist, ist eine Interaktion …