Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"GFP fusions"”.
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Functional studies of selected actin binding proteins by point mutations and GFP fusions
… C-terminus with the green fluorescent protein (GFP) with a 100-aa linker separating profilin and GFP. This fusion construct was introduced in D. discoideum profilinminus cells and expression of the fusion protein could restore the aberrant phenotype partially. The partial rescue might be due to …
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Functional Analysis of the N -Terminal Transmembrane/signal -Anchor Sequence and Linker Domain of Cytochrome P450 2C2
… region (Delta2-27) were expressed alone or as GFP fusions in E. coli and insect cells. The findings indicate that deletion of the signal anchor decreases assembly of P450 hemoprotein in E. coli but does not change the in vivo distribution of the protein. However, it alters the nature of …
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Towards an antibody mediated resistance against Geminiviruses
… in tobacco leaves as N-terminal fusion with the GFP for cytosolic expression in tobacco leaves. Additionally, two scFv-GFP fusions were targeted to the nucleus by a nuclear localization signal (NLS) introduced at the N-terminus of the scFvs. Both scFv-GFP fusion proteins were exclusively …
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MyTH4 and FERM Have Overlapping and Distinct Roles in the Function of Myo1, a Class XIV Myosin in Tetrahymena thermophila
… for MyTH4 and FERM in the function of Myo1, GFP-tagged MyTH4, FERM, and truncated FERM were separately overexpressed in Tetrahymena. Actin antibody coprecipitated tubulin, GFP-MyTH4, and GFP-FERM. GFP-MyTH4 and GFP-FERM cosedimented with either exogenous microtubules or exogenous F-actin. …
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Localization of Red Clover Necrotic Mosaic Virus Polymerase Proteins at the Endoplasmic Reticulum of Living Plant Cells
… in plants using green fluorescent protein (GFP) fusions observed in live cells using confocal microscopy. The GFP:p27 and GFP:p88 fusions were expressed in N. benthamiana epidermal cells and protoplasts. GFP:p27 consistently localized to the endoplasmic reticulum (ER) and caused membrane …
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Significance of low-abundance transcripts detected in Caenorhabditis elegans muscle SAGE libraries
… in body wall muscle. Subsequently, reporter GFP fusions were used to deduce if these low-expressed transcripts are indeed expressed in vivo within muscle. Of the low-expressed genes that tested positive via RT-PCR, 42% showed in vivo expression in body wall muscle. When the results from the …
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Characterisation of the PQ-loop repeat membrane protein family in arabidopsis thaliana
… expression was investigated using promoter::GUS fusions and quantitative PCR. Whereas AtPQL4 and AtPQL6 appear to be expressed ubiquitously, AtPQL1, AtPQL2 and AtPQL5 are all preferentially expressed in floral tissues and AtPQL3 is expressed primarily in roots. Several family members showed …
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CHARACTERIZATION OF INFLUENZA NUCLEOPROTEIN BODY DOMAIN AS ANTIVIRAL TARGET
… and Western Blot, in addition to NP-GFP fusions and fluorescence, confirm NPbd3 was expressed and localized as WT-NP. Gel shift with purified NP protein confirm NPbd3 bound nucleic acids as WT-NP. Although NPbd3 was expressed, localized, and bound nucleic acid as WT-NP, I found NPbd3 …
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Charakterisierung des Zytoskelett-Proteins Villidin und einer dritten Profilin-Isoform in Dictyostelium discoideum
… to a still unknown population of vesicles. GFP fusion proteins with the villin homology domains show a similar distribution, whereas GFP fusions of the N-terminal part encompassing the WD-repeats are present in F-actin rich regions at the plasma membrane and on internal membranes during …
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Subcellular Localization of Nicotiana tabacum TGA Transcription Factors
… sind. In dieser Arbeit wurden Fusionsproteine von TGA2.1, TGA2.2 und TGA1a mit GFP unter der Kontrolle des HBT-Promotors transient in Pflanzenprotoplasten oder stabil in transgenen Pflanzen exprimiert und direkt in lebenden Zellen über Fluoreszenz- und konfokale …