Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 756 for “"GFP"”.
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Purification of Recombinant HIS6-GFP-Preptin Fusion Protiens
… recombinant expression in Escherichia coli. The GFP-preptin fusion protein was purified using nickel affinity chromatography, followed by gel electrophoresis to confirm molecular weight and purity. The use of GFP facilitated real-time tracking of purification efficiency, with SDS-PAGE revealing a …
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Investigating Subcellular Thiol Redox Chemistry With Gfp-Based Redox Sensors
… redox-sensitive yellow fluorescent protein and roGFP, or redox-sensitive green fluorescent protein) specifically equilibrate with the local GSH:GSSG pool and register redox changes via disulfide bond formation. Redox western and fluorimeter measurements were used to demonstrate that the GSH:GSSG …
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A Dual-GFP Reporter System for Studying Bi-Directional Promotion
… transcription. Green Fluorescent Protein (GFP) reporters may be suitable for simultaneous measurement in a dualreporter system. GFP variants have similar half-lives, sensitivity for detection, and distinct excitation and emission wavelengths. The GFP variant, ECFP and EYFP, were chosen as …
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Analyzing GFP-tagged cytoskeletal protein colocalization in human carcinoma cells
… used to transfect HeLa cells with four "marker" GFP-tagged protein constructs. Cells were fluorescently labeled in three channels-Hoechst for nucleus, Texas Red phalloidin for actin, and GFP for protein-and images were captured using Cellomics scanning microscopy. After collecting data and …
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DRUG TOXICITY STUDIES BASED ON VIRE2-GFP MOBILITY INSIDE PLANT CELLS
… the ability to visualise the mobility of VirE2-GFP fluorescent protein in real time within Nicotiana benthamiana leaf cells. By monitoring the mobility of VirE2-GFP protein, we can detect toxicity of the difference between control samples and samples subjected to drugs. A total of 9 drugs which …
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Investigation of selected autophagic proteins and the effect of tPS1-GFP expression.
… proteins after the transfection, a truncated PS1-GFP construct was transiently transfected into HEK293 cells. Also, the subcellular localizations of the proteins were determined by dual label ICC.The data obtained suggests that in non-autophagic HEK293 cells, mTOR appears to be localized to …
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Entwicklung eines GFP-Reportersystems in Legionella und molekularbiologische Funktionsanalyse des Legionella Mip-Proteins
… und Wirtszelle wurde in dieser Arbeit ein GFP (Green Fluorescent Protein)-Reportersystem etabliert und erfolgreich eingesetzt. Es erlaubt ein in vivo Monitoring von Legionella Infektionen und ermöglicht die schnelle Quantifizierung bakterieller Invasion in Wirtszellen in Abhängigkeit von …
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Wechselwirkungen zwischen Chromophor und Proteinmatrix in den autofluoreszierenden Proteinen GFP und drFP583 (DsRed)
… von fluoreszenzspektroskopisch unterscheidbaren GFP-Varianten (multispektrale Detektion) erweitert werden. Eine Veränderung der spektralen Eigenschaften des Proteins unter Beibehaltung seiner Funktionalität in vivo kann nur durch Mutagenese erfolgen. Das Ziel dieser Arbeit ist es, einen Beitrag …
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Functional studies of selected actin binding proteins by point mutations and GFP fusions
… C-terminus with the green fluorescent protein (GFP) with a 100-aa linker separating profilin and GFP. This fusion construct was introduced in D. discoideum profilinminus cells and expression of the fusion protein could restore the aberrant phenotype partially. The partial rescue might be due to …
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Infection and Dissemination of TaV-GFP Tagged Sindbis in Aedine Mosquitoes and Cell Lines
… in vivo environments. The fluorescent protein GFP was inserted between the Capsid and PE2 in the genome of TR339; SINV TaV-GFP (Wm. Klimstra Lab). This virus construct should have the same infectivity and virulence as wild type TR339, leaving a fluorescent ‘path’ in infected cells that may …
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Etablierung eines Modellsystems zur zellulären und biochemischen Phänotypisierung von Prionproteinen unter Verwendung von GFP-PrP-Chimären
… die Verwendung des grünen Fluoreszenzproteins (GFP) als integrales Markerprotein in PrP eine direkte Beobachtung von PrP und PrP-Mutanten in lebenden Zellen ermöglichte. Es wurden insgesamt neben der Chimäre mit wt PrP und GFP weitere 14 Chimären hergestellt, von denen 11 mit PrP-Mutanten …
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Engineering GFP-Family Member Protiens to Achieve Novel Functions: A Class of Proteins Limited Only by the Imagination
The Green Fluorescent Protein (GFP) has single handedly revolutionized microscopy and molecular biology. A simple search for GFP in the NCBI Pubmed database yields over 29,900 unique publications as of April 2015 demonstrating the importance and influence GFP has had on the scientific community. It …
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Characterization of the Biological Activities of Recombinant Fusion Protein Green Fluorescent Protein/Human Zona Pellucida Protein 3 (GFP/HZP3)
… with human sperm. Through the application of a pEGFP expression vector, fusion protein GFP/ZP3 was successfully generated and expressed. The expression of GFP/ZP3 was evidenced by RT-PCR and western blot. The fusion protein was partially purified by Ni-NTA affinity column from cell culture medium …
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Dose Dependent Survivability of Gfp-Expressing Escherichia Coli in the Alimentary Canal of House Flies (<i>Musca domestica L.</i>)
… investigated the dose dependent survivability of GFP-expressing Escherichia coli (GFP E. coli) in the fly alimentary canal and transmissibility of each bacterial dose was assessed by culturing excreta and also from food substrate. Four different doses of GFP E. coli (very low, low, medium and …
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Expression of green fluorescent protein (GFP) in Leishmania spp. to identify survival strategies the parasite imposes on the human host
… for the first time constitutively expressing GFP in L. aethiopica, L. major, L. mexicana and L. tropica. The effect of infection on host macrophages was directly quantified via comparative proteomics which identified significant differences in protein expression not only between uninfected and …
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Development of a Human Lung Cancer Cytochrome C-GFP Reporter Cell Line to Study Anti-cancer Drug Responses in Apoptosis Pathways
… we have successfully constructed a cytochrome C-GFP reporter cell line using human lung cancer cells. The result from western blot confirmed the presence of the intact cytochrome C-GFP fusion protein in the reporter cell line. We investigated the release kinetics of cytochrome C-GFP in response …
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GFP- coated microparticles to quantify and compare wild- type desmin with known desmin mutations in human heart disease via quantitative live-cell fluorescence Imaging
… 100%) of enhanced green fluorescent protein (EGFP); and the total amount of EGFP bound per single microparticle was determined. EGFP- coated microparticles were detected and imaged by a standard epi-fluorescence inverted microscope combined withOlympus Scan^R automated image acquisition …
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MyTH4 and FERM Have Overlapping and Distinct Roles in the Function of Myo1, a Class XIV Myosin in Tetrahymena thermophila
… for MyTH4 and FERM in the function of Myo1, GFP-tagged MyTH4, FERM, and truncated FERM were separately overexpressed in Tetrahymena. Actin antibody coprecipitated tubulin, GFP-MyTH4, and GFP-FERM. GFP-MyTH4 and GFP-FERM cosedimented with either exogenous microtubules or exogenous F-actin. …
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The Potential for Green Fluorescent Protein as a Screening Tool in the Production of Haploid Potato Plants
… pHB2892 with genes for green florescent protein (GFP) and kanamycin resistance. Hemizygous primary transformants (To) were produced from three leaf discs: 17 diploid plants from one leaf disc, three and nine tetraploids from the other two leaf discs. GFP expression was observed qualitatively under …
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