Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"GCaMP"”.
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Mechanically driven In vivo Osteocyte Ca2+ Signaling: Aging and Pharmacologic Effects
… novel mouse strain that is capable of producing GCaMP signal throughout its lifespan. We used a tamoxifen-inducible GCaMP reporter mouse that only expressed GCaMP signal when needed. This tamoxifen-inducible mouse has been used in other bone research studies in mice up to 18 months of age. In …
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The Automatic Tracking Of <i>Caenorhabditis elegans</i> And Its Use In Determining Genetic Function
… for use in this technique, one in particular is GCaMP. GCaMP has undergone many rounds of targeted mutations with the aim to increase the molecule’s dynamic range and dissociation constant. At the time of commencing this project, new variants of GCaMP, known as GCaMP6s, became available, and had …
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Evaluation of the Functional Roles of TRPA1 Homologs, Painless and dTRPA1, in Chemical Nociception in Drosophila
… <italic>dTRPA1</italic> cell excitability, the GCaMP transgene was utilized to observe changes in calcium levels. Both <italic>painless</italic>- and <italic>dTRPA1</italic>-expressing cells exhibited significant changes in fluorescence following the application of AITC. Notably, it was …
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Design and Development of Multiscale Microscopic Imaging Systems for Neuroscience Applications
… efficiency of two common biosensors RGECO and GCaMP when used with an excitation wavelength of 1030 nm. The fluorescence detection using GCaMP showed improved efficiency compared to RGECO. Both microscopes are designed with cost-effective and highly efficient elements for maximum fluorescence …
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Dining in the Dark ATP Sensing in the Female Aedes Aegypti Mosquito
… blood ingestion. Previous work from the lab with GCaMP calcium imaging of stylet neurons showed that approximately 50% of stylet neurons respond to blood, and that these same neurons respond to a mixture of saline and ATP. However, ATP alone activates only a small subset of 3-7 stylet neurons …
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Development of Optical Tools and Techniques Toward a Functional Connectomic Understanding of C. elegans
… functionality of a tool to strategically arrange GCaMP in clusters, STARC, to enable imaging of compartmentalized neural activity in regions dense with neural projections. Finally, I present a novel computational and RNA fluorescent in-situ hybridization-based method for unique identification of …
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Lateral Parabrachial Choline Acetyltransferase Neurons and the Decision to Eat
… using the genetically encoded calcium indicator GCaMP. The dynamics around the decision to consume food were seen to be different between fasted and sated satiety states. Activation of the circuit showed a pronounced latency to food consumption and time to finish for a single calorie of food. …
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Novel Optical Tools to Investigate Presenilin Control of Neurotransmission
… a ratiometric version of the low affinity ER-GCaMPs in which we fused the HaloTag protein to allow for direct reporting of ER Ca<sup>2+</sup> levels. We coupled this new indicator with in-cell calibrations at physiological temperatures to create a highly robust readout of ER Ca<sup>2+</sup> …
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Progesterone Regulation of the GnRH Pulse Generator
… GnRH pulse generator using ARN kisspeptin neuron GCaMP fibre photometry in freely behaving mice. Diestrous mice equipped for monitoring the activity of the GnRH pulse generator were injected with either 4 or 8 mg/kg P4 and kisspeptin neuron synchronisation episodes (SEs) were monitored for 24 …
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Effect of hypoxia on spontaneous neural activity in the cortex of neonate mouse pups
… under normal air and hypoxic air in SNAP-25-2A-GCaMP-6s transgenic mice at postnatal day 7. Results of this study demonstrated a statistically significant reduction in frequency and increase in amplitude of neuronal activity in the entire cortex under hypoxic air when compared to normal air. …
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Effect of hypoxia on spontaneous neural activity in the cortex of neonate mouse pups
… under normal air and hypoxic air in SNAP-25-2A-GCaMP-6s transgenic mice at postnatal day 7. Results of this study demonstrated a statistically significant reduction in frequency and increase in amplitude of neuronal activity in the entire cortex under hypoxic air when compared to normal air. …
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The Taste of Blood
… a mosquito bites her victim. Using pan-neuronal GCaMP calcium imaging, we found that stylet neurons robustly respond to blood and its components but are insensitive to nectar-specific sugars. The complex mixture of blood is detected by four functionally distinct stylet neuron classes, each tuned …
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Engineering protein self-assembly for biosensing applications
… a fluorescent calcium indicator protein GCaMP into the coating, we demonstrated calcium sensing, enabling the monitoring the calcium levels in biological fluids relevant to hypercalcemia. Second, we explored the cooperative self-assembly of hybrid protein cages designed to encapsulate …
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Neural Population Dynamics Underlying Encoding of Time in the Cortico-Striatal Circuit
… to the d1St, we employed a calcium indicator (GCaMP) to label cortical neurons projecting directly to the d1St. Under two-photon microscopy, we recorded their neural activity while the animals performed the sensory timing task. Most of the labeled neurons were found in the secondary motor …
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Oxytocin, Dopamine, and the Neuromodulation of Mating Behavior in C. Elegans
… mating with the fluorescent calcium indicator GCaMP. I describe an activity pattern for DVA that coordinates calcium signal rises and falls with specific sub-behaviors within the mating behavior. I then look at DVA in nematocin deficient and dopamine-deficient males, and find that DVA activity …
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In-vivo analysis of hypothalamic kisspeptin neuron activity patterns
… populations in female reproduction using in-vivo GCaMP fibre photometry in mice. Polycystic ovary syndrome (PCOS) affects approximately one in ten women of reproductive age, often presenting with subfertility and hyperandrogenism. Another hallmark of PCOS is increased LH pulsatility, suggesting …
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A Comparative Study of Sensory Neuron Synaptic Activity and the Role of Presynaptic Diversity, Specificity, and Regulation in Caenorhabitis Elegans
… measuring synaptic calcium influx by tethering GCaMP to synaptic vesicles, and we provide our initial characterization of glutamate release in AWC<sup>ON</sup> and ASH. Our results indicate that AWC<sup>ON</sup> and ASH have distinct exocytosis dynamics and that AWC<sup>ON</sup> exhibits …
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Novel contrasts in photoacoustic tomography
<p>Photoacoustic tomography (PAT) combines rich optical contrast and high ultrasonic resolution in optically scattering tissue at depths. Taking advantage of its 100% sensitivity to optical absorption, PAT has been widely applied to structural, functional and molecular imaging, with both endogenous …
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Understanding How Sequence Differences Among Glutamate Transporters Might Contribute to their Differential Function in Glutamate Clearance in C. elegans
<p>In the nervous system, high circuit resolution requires efficient post-signaling clearance of the neurotransmitter. Glutamate-mediated neurotransmission involves the release of glutamate (Glu) into the synaptic cleft by the presynaptic cell and binding to glutamate receptors (GluR) on the …
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