Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 23 for “"GCN2"”.
-
Structural and Functional Characterisation of the Nutrient Sensing Kinase GCN2
… and when they are scarce, and adapt accordingly. GCN2 is a key protein kinase within the eukaryotic proteome, and it is activated by a drop in the intracellular concentration of amino acids. When activated, GCN2 phosphorylates the translation initiation factor eIF2, initiating the Integrated …
-
GCN2 eIF2 Kinase Promotes Prostate Cancer by Maintaining Amino Acid Homeostasis
… adaptation. We reported that the eIF2 kinase GCN2 is a critical driver of the ISR in PCa and is crucial to maintaining amino acid (AA) homeostasis. GCN2 is activated in PCa due to AA limitation, resulting in increased expression of key AA transporters which providing nutrient import to fuel …
-
Elucidating how mutations in EIF2AK4 cause pulmonary vascular disease
… mutations in the stress sensing kinase, EIF2AK4 (GCN2), are the main genetic cause of PVOD. I hypothesised that loss of GCN2 may lead to a pro-inflammatory phenotype which could be contributing to the development of PVOD. PVOD was modelled using mice with homozygous null mutations in *gcn2*. …
-
The effect of alternative splicing on key regulators of the integrated stress response
… kinase General control non-derepressible-2 (GCN2) is a key regulator of the Integrated stress response that responds to various stress signals, including nutritional deprivation. As a result of high levels of uncharged tRNAs during amino acid depletion, GCN2 phosphorylates serine-51 of the α …
-
A multi-level approach to understanding the regulation of translation initiation
… pathways that respond to cellular stresses, the GCN2 and mTORC1 pathways, sense amino acid insufficiency to inhibit translation initiation at distinct points. GCN2 is activated in response to amino acid deprivation and inhibits formation of the ternary complex, comprising elF2, GTP, and the …
-
A Study of Cellular Amino Acid Homeostasis Identifies Novel Targets, Inhibitors and Combination Therapies in Cancer
… these is amino acid insufficiency, sensed by GCN2. Rather than simply inhibiting SNAT2 or other downstream effectors of GCN2, a study was conceived to explore the sensitisation of cancer cells to a slew of kinase, transporter and metabolic enzyme inhibitors by a GCN2 inhibitor called TAP20, to …
-
A Study of Cellular Amino Acid Homeostasis Identifies Novel Targets, Inhibitors and Combination Therapies in Cancer
… these is amino acid insufficiency, sensed by GCN2. Rather than simply inhibiting SNAT2 or other downstream effectors of GCN2, a study was conceived to explore the sensitisation of cancer cells to a slew of kinase, transporter and metabolic enzyme inhibitors by a GCN2 inhibitor called TAP20, to …
-
Protein synthesis in cellular stress
… vedou k zastavení produkce proteinů. Kináza GCN2 se považuje za hlavního zprostředkovatele této odpovědi. Zastavení produkce proteinů je také doprovázeno vyšší expresí proteinů, podílejících se na boji se stresem. Tvorbě těchto proteinů předchází sada specializovaných regulačních procesů, …
-
Papel de YAP/TAZ en la respuesta de células tumorales a estímulos activadores de receptores de TRAIL
… la quinasa responsable de detectar su escasez es GCN2, cuya fosforilación activa la ruta de respuesta integrada al estrés (ISR). Tanto la activación de GCN2 como de PERK (sensor del estrés en el retículo endoplásmico) confluyen en la fosforilación de eIF2?, el elemento clave de la ISR, que …
-
Mechanisms of Adenovirus-Mediated Autophagy
… for the eIF2α pathway and more specifically the GCN2 kinase. Cells lacking GCN2 are more resistant to adenovirus-mediated autophagy <em>in vitro</em>; <em>in vivo </em>we also found these cells fail to undergo autophagy, but display more cell death. We believe that autophagy is a protective …
-
Mechanisms of Adenovirus-Mediated Autophagy
… for the eIF2α pathway and more specifically the GCN2 kinase. Cells lacking GCN2 are more resistant to adenovirus-mediated autophagy <em>in vitro</em>; <em>in vivo </em>we also found these cells fail to undergo autophagy, but display more cell death. We believe that autophagy is a protective …
-
The response of eIF2α kinases to plant virus infection
… - general control non-derepressible-2 kinase (GCN2) and protein kinase RNA-activated (PKR). The aim of this research was to further elucidate the role of eIF2α phosphorylation in plants, with a particular focus on the response of eIF2α kinases, including a putative PKR, to virus infection. It …
-
Essential Amino Acid (EAA) Regulation of Skeletal Muscle Protein Turnover with Age
… (mTOR), general control nonderepressible 2 (GCN2), eukaryotic initiation factor 2- alpha (eIF2α) and Sestrin 2 (SESN2) was measured using Western Blot analysis. RAP inhibited phosphorylation of MPS markers p70S6K1 and 4EBP1 in all treatments. Whereas the activation of GCN2 in young and old …
-
Papel del metabolismo de la glutamina en el control de la sensibilidad a trail de células tumorales de mama.
… a la apoptosis inducida por TRAIL. La quinasa GCN2, cuya activación ocurre en respuesta a la falta de aminoácidos, es responsable de la inducción de la expresión de TRAIL‐R2 a través de una vía de señalización que implica a los factores de transcripción ATF4 y CHOP. Por el contrario, la bajada …
-
Protective effect of TM-25659, known as TAZ activator, on high-fat diet-induced insulin resistance in C57BL/6J mice
… and inflammation in skeletal muscle via GCN2 pathways 1 Ⅰ. INTRODUCTION 2 Ⅱ. MATERIALS AND METHODS 8 A. MATERIALS 9 B. METHODS 10 1. Preparation of PA 10 2. Cell culture 10 3. Immunoblot analysis 11 4. RNA isolation and quantitative real-time polymerase chain reaction (PCR) 12 5. siRNAs …
-
An investigation of nutrition-dependent mRNA translation in Drosophila larvae
… I have also shown that the eiF2α kinases - GCN2 and PERK were not required for starvation-mediated suppression of translation.
-
The Unconventional Amino Acid Starvation Response of the Malaria Parasite, Plasmodium falciparum
… The canonical amino acid-starvation responsive GCN2/ eIF2α signaling pair is functionally conserved in P. falciparum, exhibiting remarkable specificity in detecting isoleucine availability, however, its activity was not essential to preserving the parasite in a growth-competent state during …
Page 1 of 2