Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 8 of 8 for “"GB1"”.
-
Inter- und intramolekulare Assoziationsreaktionen als zentrale Prozesse im Verlauf der Faltung und Stabilisierung von Proteinen
… Proteinvarianten zu erhalten. Für das Gb1-Protein aus Streptococcus sp. wurden darüber unter anderem vier stark stabilisierende hydrophobe Austausche identifiziert, die in der Variante Gb1-M2 kombiniert wurden. In der vorliegenden Arbeit wurde die Stabilität dieser hyperstabilen …
-
Developing Chemical Tools for the Inhibition of Activin A Signalling
… of activin A to design and generate a series of GB1- fused peptides based on the α1-helix-loop-α2-helix motif of the pro-domain. I determined the α1-helix-loop epitope to be key for interactions with the mature domain, before investigating the effect of truncation and mutation on this epitope. I …
-
Investigation Into Protein Folding and Misfolding
… immunoglobulin-binding domain of protein G (GB1) as our model system. Using bioinformatics approaches we investigated the origin and evolution of GB1. We also elucidated a group of conserved residues and a network of long-range interactions which we propose are key determinants in dictating …
-
Solid-State Magic-Angle Spinning NMR Methods for Tensor Measurements and Protein Structure Refinement Using Chemical Shift Tensors
… immunoglobulin binding domain of protein G (GB1). Experimental techniques presented include: three-dimensional experiments that recouple 13C or 15N chemical shift anisotropy (CSA) pseudostatic powder lineshapes, slow magic angle spinning (MAS) analysis of highly-13C,15N-enriched solid …
-
Biomolecular Solid-State Nuclear Magnetic Resonance Methods for Spectral Assignment and High-Resolution Structure Determination of Proteins
… immunoglobulin binding domain of protein G (GB1) as a model protein. Complete 13C and 15N chemical shifts are presented based on 2D 13C-13C, 15N-13C and 3D 15N-13C- 13C and 13C-15N-13C correlation spectra. Aromatic signal assignments are performed with customized experiments and processing …
-
MARKOV STATE MODELS AND THEIR APPLICATIONS IN PROTEIN FOLDING SIMULATION, SMALL MOLECULE DESIGN, AND MEMBRANE PROTEIN MODELING
… protein from c-terminal hairpin of protein G, GB1. Three mutations, tz4, tz5, and tz6 are discussed, and their folding rates and folding mechanisms are compared with wild-type GB1 using MSMs built from a significantly large MD simulation data set (aggregating over 9 millisecond). Finally, …
-
State-of-the-art REDOR and TEDOR methods for elucidation of protein structure and interactions
… 2H, 15N (U-CDN) labeled proteins, like GB1 crystalline protein and a-syn fibrils and their mutants, back-exchanged to 10-30% 1H. 1H-detected REDOR or TEDOR techniques developed here are unique in measuring distances between the side chain 13C atoms and backbone 1H atoms in the proteins, …
-
Structure determination of proteins and protein aggregates by magic-angle spinning solid-state NMR
Solid state NMR (SSNMR) is a structure determination technique uniquely suited to study protein aggregates and fibrils. Unlike solution NMR or X-ray crystallography, SSNMR can obtain atomic resolution structural information on samples of protein fibrils which are insoluble and do not produce X-ray …