Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"GATA6"”.
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The role of transcription factor GATA6 in the development of the human pancreas
… mutations. Using these tools, I found that GATA6 is required for the formation of the definitive endoderm (DE) and pancreas in humans; hPSCs harbouring homozygous GATA6 mutations fail to form the definitive endoderm, and consequently the pancreas, whereas hPSCs harbouring heterozygous GATA6 …
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Extracellular Signalling and Stem Cell Self-Renewal
… expression of the PrE transcription factor Gata6 destabilises mouse ES cell pluripotency in vitro and upregulates PrE-associated genes independently of FGF signalling. As previous studies show that PrE specification is compromised in Fgf4-/- embryos, despite initiation of Gata6, this …
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Expression of DIO2 in the Porcine Ovary and the Granulosa Cells of Humans
… is modulated by transcription factors GATA4 and GATA6. These GATA factors have been shown to regulate gene expression in the ovary. Using Stealth RNAi coupled with cDNA microarray analysis using Affymetrix Porcine Genome Arrays, our lab sought to identify genes altered by reduction of GATA4, …
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Investigating the Reconstitution and Function of Peritoneal Cavity Macrophages after LPS-induced Peritonitis
… the abdomen. Like most tissue macrophages, the GATA6+ large peritoneal macrophages (LPMs) are responsible for the clearance and removal of pathogens that may break through the defensive barriers of the abdomen. However, upon inflammation incurred by the response of the immune system to foreign …
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Investigating the transcription factor landscape of the squamous subtype of pancreatic ductal adenocarcinoma
… of human squamous PDAC cell lines. Unexpectedly GATA6 which has historically been associated with being downregulated in this PDAC subtype is also found as a TP63 interactor, and shares many TP63 and FOXA1 common ChIP binding sites. Ongoing work aims to understand the interplay between TP63, …
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Defining the minimal and context-dependent signalling cascades regulating the maintenance of human naïve embryonic stem cells and early embryo development
… manner, determined by the emergence of GATA6 cells in either IWP-L6 or DKK1(two inhibitors of WNT signalling). WNT signalling governs hypoblast- but not trophoblast-like cell differentiation in HNES cells. The blockade of FGF receptors using potent inhibitors derails differentiation and …
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The Role of NADPH Oxidase in ROS Mediated Differentiation
… study shows Nox1 and Nox4 are up-regulated when Gata6 is overexpressed in F9 cells. Furthermore, the pan-NOX inhibitor VAS2870 and NOX1-specific inhibitor ML171 significantly reduced the ability of RA to induce PrE differentiation. Additionally, a knockdown of Nox1 and/or Nox4 attenuate …
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Coordinated Blockade of TGF-β and PD-L1 Promotes Anti-Tumor Immunity in Ovarian Cancer Models
… memory T cells and decreased suppressive Gata6+ large peritoneal macrophages in the tumor, thus reshaping the tumor immune landscape in HGSC. These findings show that BA treatments can overcome immunotherapy resistance in preclinical HGSC models and promote anti-tumor immunity. This work …
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Placental co-transcriptional activator Vestigial-like 1 (VGLL1) drives tumorigenesis via increasing transcription of proliferation and invasion genes
… three cell types, including TEAD1-4, AP-1, and GATA6, and revealed ~3,000 shared genes with which VGLL1 interacts. Furthermore, increased VGLL1 expression led to an enhancement of cell invasion and proliferation, which was supported by RNA-seq analysis showing transcriptional changes in several …
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Multi-omic characterisation of Barrett’s oesophagus reveals a molecular continuum in the progression to oesophageal adenocarcinoma
… visible in indolent, ND samples. TP53 mutation, GATA6 amplification, ERBB2 amplification, APC mutation and whole genome doubling are confined to cases that have progressed with TP53 being by far the most prevalent. In contrast CDKN2A alteration occurs early in around 50% of indolent cases. SV …
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Artificial neural network and precision genome engineering frameworks for genetic system engineering in mammalian cells
… including derivatives generated using GATA6-mediated and Neurogenin-mediated directed differentiation. The sensor provides a fluorescent readout based on the activity of a specific miRNA with single-cell resolution, and has applications in organoid engineering and basic research. The …
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Supplementing Bovine Embryo Culture Media to Improve the Production and Quality of In Vitro Produced Bovine Embryos
… by IL6 was largely comprised of hypoblast (GATA6+:NANOG-) cells, and most IL6-responsive cells in day 9 blastocysts were hypoblast cells (as measured by STAT3 activation). However, some epiblast (NANOG+) cells were also IL6-responsive, and IL6 appeared to initially slow epiblast …
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MiR-10a Regulation of Drug Response and Cancer Stem Cell Populations in Non-Small Cell Lung Carcinomas
… We identified the transcription factor GATA6 as both a target of miR-10a with a predicted miR-10a target site in its 3’UTR and a regulator of PI3K expression, with several conserved binding sites in the promoter of PIK3CA. These findings demonstrate that miR-10a regulates the PI3K …
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Dietary Supplementation of Omega-3 Fatty Acids Influences the Equine Maternal Uterine Environment and Embryonic Development
… 4, relative transcript abundance of GATA4 and GATA6, markers of endoderm differentiation, along with GATA3 and ELF3, markers of trophectoderm differentiation were greater (P< 0.05) in embryos from LCPUFA supplemented mares (n=5), than controls (n=5). These results indicate that algae-derived …
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Developing a stem-cell system of embryonic development organised by a morphogen signaling centre
… Markers for the mesoderm and endoderm: SOX17, GATA6, Bra, and MIXL1 was expressed in varying levels while only gastruloids formed with a bone morphogenetic protein-4 signaling center, expressed markers for anterior neural lineages as seen by an upregulation in POU3F1, EN1, OTX2, IRX6, and …