Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"GAP activity"”.
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Developing a virtual reality information gap activity for collaborative language learning
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-10-20 without embargo terms
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Regulation of signal transduction by RGS4
… experiments indicated that RGS4S30C had enhanced GAP activity towards Go1 compared to wild type RGS4. This approach also demonstrated a simultaneous significant decrease in potency of both adrenaline and UK14304 to increase 2A-arenoceptor-activated high-affinity GTPase activity of Go1 in the …
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Mechanism of action of AexU, a new type III secretion system effector from an emerging human pathogen Aeromonas hydrophila
… (ADPRT) and GTPase-activating protein (GAP) activity. AexU was successfully expressed in the HeLa cell Tet-Off system and I provided evidence that cells expressing and producing the full length AexU showed actin reorganization followed by apoptosis. Earlier, we showed that the ÄaexU null …
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Biochemical and Biophysical Studies of Novel Features of Ras-related Protein Interactions
… structure and/or function that can lead to overactivity.</p> <p>The Ras-related protein Cell division cycle 42 (Cdc42) is important in regulating cell-signaling processes. The T35A mutation in Cdc42 leads to a decrease in flexibility of the Switch I region responsible for effector binding. The …
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Dual Regulation of Phospholipase C-beta by G betagamma
… isoforms are also GTPase activating proteins (GAPs) for Gaq, and Gbg subunits inhibit the GAP activity of PLC-b. Coordinated regulation of these activities is essential for sustained signaling at steady state. Regulation of PLC-b by Gaq and Gbg is well studied but details of the mechanism are …
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Identification of TbRP2-interacting proteins using proximity-dependent biotinylation (BioID)
… to function as a GTPase-activating protein (GAP) for tubulin, evidence now suggests that it acts as a GAP for Arl3 (a small GTPase) and together with Arl3 is involved in trafficking proteins to the cilium. I have been studying RP2 function in the flagellated protist Trypanosoma brucei, a …
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Biochemical investigation of phosphodiesterase type IV post-translational modification, cellular localisation and interaction with associated binding proteins
… protection from ERK-mediated inhibition of PDE4 activity. SUMOylation alters the association of PDE4 isoforms with binding partners like β-Arrestin, AKAP18 δ and UBC9. Rolipram is an archetypal PDE4 specific inhibitor. In this study it is shown that in cells expressing a GFP tagged form of PDE4A4 …
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Dissecting the Function of FARP1 and Rho GTPases in Semaphorin-Plexin Signaling: Structural Perspective
… of Plexin and stimulates its cytoplasmic GAP activity towards Rap, resulting in cytoskeleton re-organization. The growth cone of an axon then turns around, which ultimately leads to the repulsive guidance of the axon. Structural studies by our laboratory and others have revealed how the …
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Genetic Analysis of Grinder Formation in Caenorhabditis Elegans: Regulation by RAB-6.2 and Its GTPase Activating Protein EAT-17
… GDP bound states. GTPase activating proteins (GAPs) catalyze the hydrolysis of GTP, allowing Rabs to be extracted from membranes and recycled for additional rounds of signaling. I found that the GAP activity of EAT-17 is important for its function: 21% (16/76) of mutants expressing a wild-type …
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Amino acid regulation of mTORC1
… based signaling platform that controls the activity of the Rags. We find that Ragulator is a pentameric complex that is both necessary and sufficient to determine the intracellular localization of Rags. Moreover, we find that Ragulator functions as a guanine nucleotide exchange factor (GEF) …
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RacGap50C Is a Novel Negative Regulator of Wnt Signaling
… epidermal patterning carry mutations in the RacGap50C gene. These mutations on their own cause cuticle pattern disruptions that include replacement of ventral denticles with naked cuticle, which indicate that the Wg pathway is ectopically activated in mutant embryos. In addition, RacGap50C …
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Studies on the Molecular Mechanisms of Tumorigenesis in Neurofibromatosis Type 1
… neurofibromin, a tumor suppressor with Ras-GAP activity that negatively regulates p21-RAS signaling. Neurofibromin deficiency typically causes chronic activation of Ras, which is thought to be a major contributor to manifestation of NF1. The cardinal features of NF1 are cafe-au-lait (CAL) …