Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 73 for “"GAL4"”.
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Identification and Characterization of Two Novel Sleep Promoting Neurons Located in the Ventral Nerve Cord of Drosophila Melanogaster
… sleep. In the Drosophila sleep field, the 23E10-GAL4 driver is the most widely used tool to modulate sleep. 23E10-GAL4 expresses in the dorsal Fan-Shaped Body (dFB), a brain region whose associated neurons are believed to be a major sleep regulating center. However, because the dFB contains a …
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Transcriptional Regulation of an Extrachromosomal Gal7 Gene in Saccharomyces Cerevisiae (Yeast Plasmids, Regulatory Mutants, Over Expression)
… repression by glucose or control by positive (GAL4) or negative (GAL80) regulatory factors. Increasing the gene dosage to more than 200 copies per cell increased the galactose induced accumulation of GAL7 mRNA more than 10 fold, reduced galactose induced expression of the single copy …
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Studies on the mechanisms of action of the male specific lethal complex in Drosophila
… of the complex were combined with the yeast GAL4 binding domain. When targeted to a UAS-minimal promoter mini-white reporter, which was recovered at several X and autosomal positions, GAL4-MOF causes up-regulation in females but down-regulation in males. By using immuno-staining and FISH, it …
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Lokalisierung eines Gedächtnisses bei Drosophila melanogaster
… rutabaga in rut-mutanten Fliegen mit Hilfe des Gal4/UAS-Systems ausschließlich in den Pilzkörpern, bzw., im Gegenexperiment, nur außerhalb der Pilzkörper zur Expression gebracht. rut kodiert für die Adenylatcyclase I, die mit synaptischer Plastizität bei Drosophila, Aplysia und Mäusen in …
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Analysis of the Molecular Mechanisms of Drosophila Antennal Lobe Development
… In rescue experiments, where cell-type specific GAL4 drivers were used to drive Drl expression in drl null mutant background, the drl mutant phenotype is rescued by both Repo-GAL4, a glial-cell specific driver and GH146-drl, where the regulatory region of GH146-GAL4, a PN driver is directly fused …
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Regulation of growth, patterning and cell fate-specification during imaginal disc regeneration in drosophila
… fate to eye fate induced by a Distal-less-GAL4 (DllGAL4) P-element insertion that is a mutant allele of Dll and expresses GAL4 in the antennal imaginal disc. While this fate change was not induced by tissue damage, it appeared to be a hybrid of transdetermination and homeosis. This …
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OMB and ORG-1
… transgenic flies and a number of different Gal4 driver lines. Misexpression of org-1 during imaginal development interfered with the normal development of many organs and resulted in flies with a plethora of phenotypes. These include a homeotic transformation of distal antenna (flagellum) …
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Analysis of Tribolium head patterning by forward and reverse genetics and transgenic techniques
… Ziel meiner Arbeit das binäre Expressionssystem GAL4/UAS für Tribolium zu etablieren. GAL4 wird durch Hitzeschock aktiviert, indem der Tribolium hsp68 promotor verwendet wird. Nach dem Hitzeschock wird das Reportergen über das UAS Konstrukt sowohl während allen Stadien der Triboliumentwicklung …
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The roles of spartin in Drosophila circadian clock
… attenuated. Nonetheless, we observed that c155-gal4;;spartin1 flies manifested~24 h periodalbeit with no expression of spartin. On the contrary, (17)Da-gal4;spartin1 flies manifested~26.7 h period with strong expression of spartin. These results indicated that there is no correlation between …
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Biological Consequences of Polyglutamine Repeats in Drosophila Muscle
… polyQ disease. Here, we utilize the UAS/GAL4 system to express a pathogenic HD construct in the muscle of the fly, and characterize the effects. We observe detrimental phenotypes like reduced lifespan, decreased locomotion, and accumulation of protein aggregates. Interestingly, the …
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Development of Tools to Assess the Effects of Lunasin on Normal Development and Tumor Progression in Drosophila Melanogaster
… accomplished by crossing transgenics to various GAL4 driver lines. Progeny were assessed for phenotypic alterations and no phenotypic abnormalities were observed in tissues expressing EGFP-Lunasin, supporting current studies that show Lunasin does not affect normal cells. Previous studies have …
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Genetic Analysis of The Function of The Drosophila Doublesex-Related Factor Dmrt93B
… knockdowns. In order to disrupt this gene, the GAL4 yeast transcriptional activator followed by a polyadenylation signal was inserted after the dmrt93B start codon and introduced into the genome by homologous recombination. Analysis of the knock-in mutation as well as a small deletion removing …
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Controlling Gene Expression with Synthetic Molecules
… HeLa cells a luciferase reporter gene harboring Gal4 binding sites and a construct in which the ligand binding domain of the Glucocorticoid receptor has been fused to Gal4 DBD. The cells are treated with the CBP-binding peptoids that have been chemically coupled to a dexamethasone derivative. …
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Die transkriptionelle Regulation des roughest Gens in Drosophila melanogaster
… identifiziert und durch die Konstruktion von rst-Gal4 Linien charakterisiert. Hierdurch kann gezeigt werden, dass das rst Gen durch eine komplexe Anordnung von Enhancer-Modulen reguliert wird. Unter anderem sind spezifische rst cis-regulatorische Sequenzen identifizierbar, die eine Expression in …
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An investigation of the relationship between levels of apoptosis during aging and longetivity
… genotypes that overexpress DIAP1 using the DJ694-GAL4 driver, DIAP1 expression and caspase activity were examined by way of western blots and caspase assays, respectively. Second, the hypothesis was also tested through the manipulation of DIAP1 expression levels. The number of the UAS-DIAP1 …
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Inducible Gene Expression in Cell Cultures and in Microencapsulated Cells
… human progesterone receptor fused to the yeast GAL4 DNA-binding domain and the <em>Herpes simplex</em> virus protein VP 16 activation domain. The third component is a synthetic promoter, consisting of a series of GAL4 recognition sequences upstream of the adenovirus major late E1B TATA box, …
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Bedeutung der Homöodomäne des Transkriptionsfaktors Pax6 für die Aktivierung des Glukagon-Gens durch Pax6
… steigert die transkriptionelle Aktivität eines GAL4-Pax6-Fusionsproteins an einem Glukagon-Promotor-Reporterfusionsgen, in dem die Pax6-Bindungsstellen im G1- und G3-Element in Bindungsstellen für GAL4 mutiert sind, wie Cotransfektionsstudien zeigen. Demnach übt die Homöodomäne in diesem Fall …
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A Role for Glycogen Synthase Kinase 3 beta in the Regulation of Glucagon Gene Transcription by Insulin
… SB-216763 aufgehoben. Dies wurde in einem GAL4-System gezeigt. Rekombinantes GSK3b phosphorilierte die Transaktivierungsdomäne von Pax6 und ein CBP-Fragment, das die Aminosäuren 2040-2305 enthielt. Dies konnte durch in vitro Kinase assays gezeigt werden. Die Ergebnisse zeigen, das GSK3-b in …
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Proteasome Inhibition Enhances The Effect of Bikdd Gene Therapy
… into a highly amplified “VISA” (VP16-GAL4-WPRE integrated systemic amplifier) system under the control of a cancer specific promoter, previous studies have verified the specific killing effect of BikDD in cancer cells both in <em>in vitro</em> and <em>in vivo</em> models. C-VISA-BikDD …
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Isolation and partial characterization of cDNAs encoding polypeptides that interact with the capB gene products of Dictyostelium discoideum
… CABP1 encoded by the capB homolog capA. The GAL4 two-hybrid system was used to screen a Dictyostelium discoideum cDNA expression library to identify proteins that interacted with p34/31. The clones were tested to eliminate false positives. DNA sequencing analysis revealed that the cDNAs …
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