Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"GABARAP"”.
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Studies on the autophagy gene WIPI4 and its interactor UBR5
… This thesis explored the regulatory mechanism of GABARAP by WIPI4. GABARAP is a potential closure regulator and is also required for expansion and fusion steps of autophagy. I identified that WIPI4 regulates the stability and trafficking of GABARAP. Altogether, this thesis furthers the …
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Autophagy and EVs in cancer cells. Effect of ATG8 knockdown on the biogenesis of CD81+ extracellular vesicles.
… or knockout of its sub-families LC3 and GABARAP, impacts the number and size of EVs. The sub-objective was to positively identify EVs as the isolated particles by examining protein markers specific to EVs and imaging the vesicles with electron microscopy. HeLa cancer cell lines were used …
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Biochemical and Structural Characterization of the Atg8/LC3 Lipidation Pathway
… for expressing and purifying human LC3 or GABARAP, ATG7, ATG3, and the ATG12–ATG5-ATG16L1 complex for in vitro studies of LC3/GABARAP lipidation; based on these protocol established, we report that an Atg3 element we term E123IR (E1, E2, and E3-interacting region) is an allosteric switch, …
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ATG8 Regulation and Function in Non-Canonical Autophagy
… this pathway, ATG8 subfamily proteins (LC3s and GABARAPs) are recruited to double membrane autophagosomes where they facilitate substrate capture, membrane biogenesis and maturation. LC3 proteins can also be recruited to single membrane, endolysosomal compartments through a distinct pathway known …
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COMPUTATIONAL DESIGN OF PEPTIDES AND PEPTIDOMIMETICS AS POTENTIAL THERAPEUTIC AGENTS TO SATISFY PRESSING MEDICAL NEEDS
… with the autophagy machinery in which GABARAP protein is involved. Finally, the machine learning weaponry was used in order to identify peptides able to inhibit WWP1 protein, a new promising target of anticancer drugs (case study 4) for which no drug candidates have been reported to …
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Decompositions of Free Energies in Molecular Simulation
… about the binding conformation of two proteins GABARAP and the GABA-A receptor. In chapter 6 statistics from hydration sites around hundreds of proteins are analysed. The distributions of free energy are shown and discussed for hydration sites in a range of local chemical environments. Also in …