Global ETD Search
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Showing 1 to 17 of 17 for “"G4S"”.
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Investigating the interactions of G-quadruplexes with transcription and chromatin structure
Four stranded G-quadruplexes (G4s) are non-canonical nucleic acid secondary structures that are primarily found in nucleosome-depleted promoters and mark sites of elevated transcription within the human genome. Although numerous studies have indicated an association between G4 formation and active …
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DNA G-quadruplex structures in human cancer cells
… and diseases. In particular, G-quadruplexes (G4s), four-stranded structures formed in guanine-rich sequences of cellular chromatin, are implicated in transcription, replication and genome stability. G4s may also present new opportunities for targeting in anti-cancer therapeutic interventions …
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Single-molecule G-quadruplex visualisation in live cells and 3D chromatin
… protein binding sites. However, the roles of G4s in biology remains elusive. Whilst G4s can exist in a folded or unfolded state, methods are required to ensure that endogenous G4 detection does not perturb G4 folding dynamics. In this work, G4-specific fluorescent probes are reported which …
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Investigating DNA G-quadruplex structure function through genome and epigenome engineering
… such as four-stranded DNA G-quadruplexes (G4s), in genome function remains a largely unanswered question. G4s are implicated in transcriptional regulation; however, earlier studies are mostly correlative and do not directly address the role of an individual G4 within its endogenous cellular …
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Development of nanobodies as tools to investigate G-quadruplex DNA secondary structures
… secondary structures called G-quadruplexes (G4). G4s are suggested to be involved in key genome functions such as transcription, genome stability and epigenetic regulation, together with numerous connections to cancer biology. The detection G4s in cells has been facilitated by the use of …
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G-Quadruplexes As Molecular Targets For Transcriptional Control
DNA G-quadruplexes (G4s) have gained interest in modern drug discovery efforts. The majority of G4 research, including the mass of this research, heavily focuses on cancer therapeutic development, as G4s are more pronounced in oncogenic promoters compared to the rest of the genome, our efforts …
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Investigating the function of DNA G-quadruplex structures in human enhancers
… molecular structures. DNA G-quadruplexes (G4s) are four-stranded DNA three-dimensional (3D) structures, that form in guanine-rich regions of the genome. G4s are enriched at sites of important regulatory potential in diverse genomes, including promoters and enhancers. Enhancers interact with …
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Nucleic acid clamp-mediated transcriptional and translational modulation of oncogenes via the stabilization of G-quadruplexes
G-quadruplexes (G4s) are non-canonical secondary nucleic acid structures that exit in various biologically important regions within a cell, such as the promoters of DNA, 5’-Untranslated regions (UTR) of mRNA and telomeres. These secondary structures tend to cluster around 1 kb from transcriptional …
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Exploring genetic interactions with G-quadruplex structures
… aims to investigate this. Stabilisation of G4s by small molecules can perturb G4-mediated processes and initial studies suggest that this approach has chemotherapeutic potential. I therefore also aimed to identify cell genotypes sensitive to G4-ligand treatment that may offer further …
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Development of Two Novel Families of Platinum(Ii) Coordination Complexes for Nucleic Acid Recognition
… architectures, such as guanine quadruplexes (G4s). These four stranded assemblies have risen to prominence as promising drug targets as a result of their demonstrated relevance and enrichment in genomic regions involved in cancer, neurodegenerative conditions, bacterial infections, and viral …
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Investigation of Genomic Instability Induced By G-Quadruplexes In The Absence of Functional to poisomerase 1 In Yeast
… cells. Thus, future attempts at enumerating G4s in <em>TOP1</em>-deletion yeast cells should include the usage of expressed BG4 fused to a nuclear localization signal sequence or purified BG4 protein. Top1 mutants arise in cancer cells treated with the Top1-targeting anticancer drug …
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Genetic selection of cyclic peptide G-quadruplex ligands
… structures. There is increasing evidence that G4s present new opportunities in treating cancer and neurodegenerative disease, but a deeper understanding of the mechanistic logic behind G4 function is necessary to exploit this secondary structure as a clinical target. The generation of new, …
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Antibody phage-displayed libraries derived from chicken immunoglobulin genes : a source of highly specific diagnostic antibodies
… the other with a 15 amino acid flexible linker [(G4S) 3] were constructed using pooled splenic RNA. The single amino acid-linked scFv repertoire was evaluated as a source of highly specific diagnostic antibodies by panning against each of the five different antigens. After two rounds of panning, …
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G4-RESOLVASE 1 TIGHTLY BINDS AND CATALYTICALLY UNWINDS UNIMOLECULAR G-QUADRUPLEX STRUCTURES WITH IMPLICATIONS FOR GENE TRANSCRIPTION AND TELOMERE BIOLOGY
G-quadruplexes (G4s) are four-stranded structures formed by guanine-rich DNA or RNA sequences in which the strands can be orientated in parallel, antiparallel, or mixed parallel/antiparallel orientations. It has been previously shown that the DHX36 gene product, G4-Resolvase1 (G4R1), binds parallel …
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Vulnerability to G - Quadruplexes in BRCA2 - Null Medulloblastoma: A Protective Role for the PIF1 Helicase
… phenotypes of PIF1 deficiency, indicating that G4s that are unable to be resolved can lead to deleterious outcomes such as the development of micronuclei and aberrant mitoses. In Brca2-/-cells, G-quadruplexes represent a hurdle to normal replication that must be resolved by helicases like PIF1 …
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Characterizing and targeting the genomic consequences of ATRX deficiency in glioma
… apoptosis, and significant induction of G4s, replication stress, and DNA damage in CX-5461-treated tumors, both alone and in combination with IR. Furthermore, systemic CX-5461 treatment induced tangible pharmacodynamic effects in ATRX-deficient intracranial GSC models, despite suboptimal …
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Migrant Surveillance and State Power: Electronic Tagging of Asylum-Seekers in the UK
While the electronic tagging of asylum-seekers is often considered a humane alternative to detention, it is actually a tactic of Theresa May’s ‘hostile environment’ that deeply constricts an individual’s physical, psychological, and social freedom. This study focuses on the experiences of …