Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"G2/M checkpoint"”.
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Mathematical Modeling Reveals That G2/M Checkpoint Override
<p>Cell cycle checkpoints determine whether cells meet requirements to progress through the next stage. In response to DNA damage, how cells activate checkpoints have been well studied, but little is known about checkpoint deactivation (recovery), which directly impacts on cell fate. In tumor …
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The Tumor Suppressor PKCδ Is Critical for UV-Induced G2/M Checkpoint Activation and Apoptosis
… expression of PKCδ-cat caused a pronounced G2/M cell cycle arrest in both primary human KCs and immortalized HaCaT cells. Consistent with a G2/M arrest, PKCδ-cat induced phosphorylation of Cdk1 (Tyr15), a critical event in the G2/M checkpoint. Treatment with the ATM/ATR inhibitor caffeine …
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Mathematical Modeling Reveals That G2/M Checkpoint Override Creates A Therapeutic Vulnerability In Tsc2-Null Tumors
<p>Cell cycle checkpoints determine whether cells meet requirements to progress through the next stage. In response to DNA damage, how cells activate checkpoints have been well studied, but little is known about checkpoint deactivation (recovery), which directly impacts on cell fate. In tumor …
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Mathematical Modeling Reveals That G2/M Checkpoint Override Creates A Therapeutic Vulnerability In Tsc2-Null Tumors
<p>Cell cycle checkpoints determine whether cells meet requirements to progress through the next stage. In response to DNA damage, how cells activate checkpoints have been well studied, but little is known about checkpoint deactivation (recovery), which directly impacts on cell fate. In tumor …
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G2/M checkpoint associated repression of polo-like kinase-1 mediated by the tumour suppressor, p53
… Kinase-1 (PLK1) is an important mediator of the G2/M phase of the cell cycle that is down-regulated in a DNA damage dependent manner. In cancer cells PLK1 is overexpressed and allows for continued proliferation of the cell by overriding this checkpoint. Here I show that PLK1 is down-regulated in …
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Characterisation of the molecular complexes that regulate the G2/M checkpoint of the eukaryotic cell cycle
… steps, comprising the interphase (G1, S and G2) and the mitotic phase (mitosis and cytokinesis), with two major regulatory checkpoints at G1 and G2. The focus of this research is the G2 checkpoint, which ensures the successful achievement of DNA replication, prior to the initiation of …
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Role of retinoblastoma protein in the development of liver cancer
… E is found to lack governance. Analysis of the G2/M checkpoint proteins such as Gadd45a, MAD2 and CDC25a all show a dysregulation in their activities. In conclusion Rb loss leads to aberrant regulation of p53 and induces DNA damage, this superimposed on a background of a lack of cell cycle …
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Harnessing mutagenic homologous recombination for in vivo targeted mutagenesis by TaGTEAM
… in this context because of DNA damage checkpoint activation and base pair lesions and use this knowledge to shift the primary mutagenic outcome of targeted endonuclease breaks from HR-independent rearrangements to HR-dependent point mutations. TaGTEAM was applied to the problem of …
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Identification of TICRR, a novel checkpoint and replication regulator
… cells have evolved surveillance mechanisms, or checkpoints, that protect the cells from DNA lesions. Understanding the molecular mechanisms by which checkpoints act is of clinical relevance, since mutations in checkpoint components are often associated with human developmental disorders and …
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Identification of Intracellular Signaling Pathways Regulated by the TAO Family of Mammalian STE20p Kinases
… is made selective and efficient. The UV-induced G2/M checkpoint is diminished when TAO kinase expression levels are reduced by siRNA. Finally we show that TAOs may be substrates of the ataxia telangiectasia mutated (ATM) and ATM and Rad50-related (ATR) DNA damage kinases, as activation of TAO2 is …
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An investigation into BK Polyomavirus and host-virus interactions
… revealing that BKPyV infection induces a pseudo-G2 arrest, similar to the G2/M checkpoint. Validation of TMT results in both cell types provided confidence in this robust data set, and further studies highlighted the importance of not only cell cycle status, but the activity of CDK1 for efficient …
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Deciphering The Role of miR-506 in Ovarian Cancer Drug Sensitivity
… impairing the signalling cascade responsible for G2/M checkpoint activation upon DNA damage insults. This behaviour, recapitulating a BRCAness phenotype, would allow propagation of cells with unrepaired DNA damage with the subsequent sensitisation to DNA damaging drugs. Furthermore, miR-506 …
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Role of Chk1 and Chk2 in mitotic checkpoint control in vertebrate cells
… genotoxic stress and mediate multiple cell cycle checkpoint mechanisms that ensure genomic integrity. The establishment of mitotic checkpoint delay in response to DNA damage or incompletely replicated DNA is conventionally thought to be accomplished through inhibition of the cyclin-dependent …
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Integration of ATM, ATR, and DNA-PKcs Signaling Maintains Genome Integrity During Neurogenesis
… induced p53-dependent apoptosis, and exacerbated checkpoint activation after ionizing radiation (IR) in a developmental stage and neural cell type-specific manner, independent of ATM and ATR. Our data suggests, during neurogenesis DNA-PKcs functions as a component of the DNA-PK holoenzyme to …