Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"G2/M arrest"”.
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The Tumor Suppressor PKCδ Is Critical for UV-Induced G2/M Checkpoint Activation and Apoptosis
… expression of PKCδ-cat caused a pronounced G2/M cell cycle arrest in both primary human KCs and immortalized HaCaT cells. Consistent with a G2/M arrest, PKCδ-cat induced phosphorylation of Cdk1 (Tyr15), a critical event in the G2/M checkpoint. Treatment with the ATM/ATR inhibitor caffeine …
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The Role of Sphingosine Kinase 2 in Cell Growth and Apoptosis
… In addition, downregulation of SphK2 decreased G2/M arrest in response to doxorubicin. Surprisingly however, siSphK2 markedly enhanced apoptosis induced by doxorubicin in MCF7 and HCT-116 cells. This result raises the question of how overexpression of SphK2 decreases cell growth and enhances …
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SIK2 Inhibitor GRN-300 Enhances Sensitivity to Paclitaxel Treatment in Triple-Negative Breast Cancer
… CDC27, CDK1, and PLK1, resulting in enhanced G2/M arrest and apoptosis. Notably, GRN-300 retained activity in paclitaxel-resistant TNBC cell lines, suggesting the potential to overcome acquired resistance. Together, these findings establish GRN-300 as a promising first-in-class SIK2 inhibitor …
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Molecular Mechanism of Parvovirus Infection
… apoptosis pathway is identified. In addition, a G2/M arrest of cell cycle during MVC infection is caused by the viral genome. Results in the second part represent the first attempt to explore the role of Epo/EpoR/Jak2 signaling during B19V infection. It not only establishes a direct connection of …
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Characterisation of Androgen Receptor Variants in Breast Cancer and the Development of Thieno[2,3-b] Pyridines as Novel Anti-Cancer Compounds
… drugs were found to induce multi-nucleation, G2/M arrest and promote apoptosis. Further, a drug pull-down assay suggested that the compounds bind to multiple targets involved in, for example, cytoskeleton dynamics and p53 signaling. In conclusion, these compounds represent a novel therapeutic …
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G2/M checkpoint associated repression of polo-like kinase-1 mediated by the tumour suppressor, p53
… Kinase-1 (PLK1) is an important mediator of the G2/M phase of the cell cycle that is down-regulated in a DNA damage dependent manner. In cancer cells PLK1 is overexpressed and allows for continued proliferation of the cell by overriding this checkpoint. Here I show that PLK1 is down-regulated in …
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Polyploidy and Mitotic Cell Death are Two Distinct HIV-1 Vpr-Driven Outcomes in Renal Tubule Epithelial Cells
… in renal tubule epithelial cells (RTECs) induces G2 arrest, apoptosis and polyploidy. The ability of a subset of cells to overcome the G2/M block and progress to polyploidy is not well understood. Polyploidy frequently associates with a bypass of cell death and disease pathogenesis. Given the …
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Chemical Genetic Studies of Tumor Suppressors in the Developing Zebrafish
… mutant standstill. This mutant suffers from a G2/M arrest due to activation of the checkpoint protein ATM such that ATM knockdown or inhibition rescues the cell cycle. Additional knockdown studies reveal that the unique zebrafish cdc25 gene cdc25d is responsible for cell cycle progression in …
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Overcoming Resistance to CDK4/6-targeted Therapy Using JAK2/STAT3 Inhibitor in Triple-Negative Breast Cancer
… synergy, i.e., inducing apoptosis, promoting G2/M arrest, reducing cellular senescence, suppressing cancer stemness, and attenuating multi-organ metastases. These preclinical findings could pave the foundation for eventual clinical evaluation of a novel combination therapy with two …
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THE ROLE OF THE TRANSCRIPTION FACTOR DEAF-1 IN PROSTATE FUNCTION
… in PC3 cell lines leads to a increase in G2/M arrest suggesting altered levels of DEAF-1 in cancer may lead to disruption of growth arrest and cell cycle check points. Finally, cDNA microarray comparison of DEAF-1 overexpressing PC3 cell lines reveals increased expression of the …
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Investigating the role of the Renin Angiotensin System in cancer
… have anticancer effects with DIZE inducing a G2/M arrest in cancer cells. We also investigated associations between drugs targeting RAS and current chemotherapeutic agents, Cisplatin (CDDP) and Doxorubicin (DOX). Our data shows that ACE-1 axis inhibitors have an antagonistic effect on CDDP, …
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Microrna Regulation of Prostate Cancer Stem/Progenitor Cells and Prostate Cancer Development
… with miR-34a mainly inducing G1 cell-cycle arrest followed by cell senescence and let-7 inducing G2/M arrest. MiR-301, on the other hand, exerted a cell type dependent effect in regulating prostate CSC properties and PCa development. In summary, our work reveals that the prostate CSC …
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Regulation of cellular growth and identification of stromal gene signatures in breast cancer
… cell growth, induced apoptosis and cell cycle G2/M arrest, which might be due to regulation of the anaphase-promoting complex protein Cdc23. The tumor is not a homogenous compartment, but consists of various different cell types, both among the cancer cells as well as in the surrounding stroma. …
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Translocations of Actin to Nucleus and p21WAF1 to Cytoplasm after Treatment of HDF Cells with H2O2
… SA-β-gal의 활성을 나타내었다. 3. H2O2를 한 번 처리한 경우에는 일시적인 G2/M 정지를 보이는 반면 반복적인 H2O2의 처리는 지속적인 G2/M 정지현상을 보였다. 4. 노화의 마커로 보고된 actin의 핵 내 이동현상은 산화적 스트레스의 일종인 H2O2를 처리한 경우에도 관찰되었으며, 이때 핵 내 축적된 actin이 단량체 actin임을 알 수 있었다. 6. H2O2에 의해 유도되는 p21WAF1의 발현은 노화가 진행된 세포보다는 젊은 세포에서 H2O2 처리 횟수에 비례하여 발현양이 증가하는 것을 관찰하였다. …
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Isolation and Functional Mapping of Human T-Cell Leukemia Virus Type 1 Tax Oncoprotein DNA-Damage Complexes
… siRNA has no significant effect on Tax-induced G2/M arrest. Tax shows no significant effect on cellular end-joining repair as measured by a plasmid-based <em>in vivo</em> end-joining assay. Tax-expressing cells show delayed dissolution of damage-induced nuclear speckles containing DNA-PK, …
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Characterizing and targeting the genomic consequences of ATRX deficiency in glioma
… activated p53-independent apoptosis, and induced G2/M arrest selectively in ATRX-deficient GSCs. These data were corroborated <em>in vivo</em>, where combinational treatment profoundly delayed tumor growth and prolonged survival exclusively in mice bearing ATRX-deficient GSC flank xenografts. …
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The discovery and anticancer preclinical investigation of novel piperazinylpyrimidine derivatives designed to target the human kinome
… the induction of both time and dose independent G2/M arrest and dose dependent apoptosis. Globally studying the molecular events accompanied with the 15 /MDA-MB-468 incidence has revealed the phosphorylation of TP53 and the consequent activation of its transcriptional activity as a hallmark …
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Komplexkonjugate bioaktiver Flavonoide & Naphthochinone und Photoaktivierung von Carboplatin für Studien der intrazellulären Verteilung
… 122 und 126 zeigten jeweils einen ausgeprägten G2/M-Arrest im Vergleich zu den entsprechenden Liganden 23, 13 und 12.36 Mit Hilfe des Woundhealing-Assay konnte die migrationshemmende Eigenschaft der Cu(II)-Komplexe dargelegt werden, wobei 122 den größten Effekt zeigte.36 Anhand der Färbung von …
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COP9 signalosome subunit knockdown in K562 provides novel insight into the function and potential regulation of the CSN
… knockdown was associated with mitotic defects, G2/M arrest, and culminated in apoptosis. In contrast, CSN2 knockdown resulted in autophagy inhibition and non-apoptotic cell death. This is the first time the CSN has been associated with autophagy. CSN2 and CSN5 knockdowns also had divergent …
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Characterization of novel small molecule inhibitors of FOXM1 as potential therapeutic agents for breast cancer
… regulator of the cell cycle, including G1/S and G2/M transition, execution of S-phase and mitotic processes, DNA damage repair, and apoptosis were impacted. Consistent with this, treatment of breast cancer cells with our inhibitors results in G2/M cell cycle arrest and apoptosis via caspase 3/7 …