Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"G1 arrest"”.
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Cell Cycle Regulation by Post-Translational and Post-Transcriptional Mechanisms in an Anaerobic Extremist- the Anoxia Tolerant Turtle, Trachemys Scripta Elegans
… and post-translational mechanisms of cell cycle arrest during anoxic stress in the freshwater turtle.Results indicated a general regulation of critical cell cycle components, in addition to the possible regulation by signaling cascades (Akt/GSK-3β and ATR/Chk2) that are known to regulate G1/G0 …
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Role of MDM2 In Cell Growth Regulation
MDM2 has been shown to induce G0-Gl/S phase arrest. To determine the cell cycle step targeted by MDM2, flow cytometry was employed to detect induction of events during the G1-S phase transition in MDM2-arrested cells. MDM2 overexpression does not prevent expression of cyclin D, cyclin D-CDK …
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Characterisation of the effects and mechanism of action of rapamycin and genistein on acute myeloid leukemia using high-throughput techniques
… induce apoptosis but effected a time-dependent G1 arrest, with the peak inhibitory effect at 16 h. Interestingly, rapa down-regulated IGFBP2, usually elevated in AML patients. Our study showed that rapa represses Skp2, an important constituent of the protein ubiquitination pathway. Working on …
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The role of the histone variant H3.3 and its chaperones in the response to DNA damage
… cycle distribution after UV irradiation, with a G1 arrest after 24 hours. These observations support the hypothesis that there is a delay in resolving DNA damage in the absence of H3.3. The observed checkpoint activation is mediated by p53, as demonstrated by alleviation of the G1 arrest in …
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Targeting Apoptotic Pathways to Overcome Drug Resistance In Acute Myeloid Leukemia
… confer tumor resistance by promoting reversible G1 arrest. Importantly, BCL-2 inhibition switches the outcomes of p53 activation from pro-survival G1 arrest to apoptosis, thus overcoming AML resistance to p53 activation. The combination of BCL-2 inhibition and p53 induction markedly prolongs …
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Characterization of the molecular mechanisms underlying retinoic acid induced growth inhibition in MCF-7 human breast cancer cells.
… be associated with an accumulation of cells in G1 phase of the cell cycle. Since the molecular mechanisms underlying these antiproliferative effects were unknown, we investigated the effects of retinoic acid (RA) on cell cycle progression and the expression and activity of cell cycle regulatory …
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P16/INK4a controls the morphology program associated with cellular senescence
… kinds of stress. The senescence program involves G1 arrest accompanied by dramatic changes in cell function and morphology. The molecular mechanisms that govern these changes are at this point only partly understood. <br>Results: We here demonstrate that the p16 protein controls the morphological …
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Strategies for the Selective Treatment of Cancer, Including the Identification and Mechanism of Action of Compounds That Induce G1 Cell Cycle Arrest
… apoptosis in melanoma cells after causing growth arrest in the G1 phase of the cell cycle. Compounds that induce G1 arrest have potential to avoid many inherent resistances and potentially have a novel macromolecular target since few G1 signaling pathways have been validated as anticancer targets. …
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In vivo and in vitro applications of biospectroscopy : potential use as a diagnostic tool which can provide insights into cell cycle dependent proliferation
… of 10-6M B[a]P causes short-term evasion of G1 arrest and accumulation in S phase. These findings indicate that growth kinetics in vitro are important pre-determinants of MCF-7 cell susceptibility to genotoxic agents. Biospectroscopy and computational analysis produced biochemical signatures …
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Activation of the p53 pathway in combination with photon irradiation for the treatment of neurological tumour cells
… AMG232 induced a stronger and more sustained G0/G1 arrest in TP53-wild-type cells, supporting activation of the canonical p53–p21 axis. TP53-mutant cells displayed only partial or transient G0/G1 accumulation, suggesting the involvement of p53-independent stress responses rather than effective …
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Targeting Autophagy to Improve Efficacy of Cdk4/6 Inhibition In Breast Cancer
… palbociclib mediated CDK4/6 inhibition induces G1 arrest and senescence in ER+ breast cancer cells, and a recent study in fibroblasts implicated a role for palbociclib in inducing autophagy, a catabolic process that facilitates survival of the cells under stress.</p> <p>Thus, we hypothesize that …
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Regulation der humanen Aurora-A Kinase und Identifikation potentieller Interaktionspartner
… Depletion of KIAA1741 by siRNA induced a G1 arrest. Interestingly KIAA1741 was a good in vitro substrate of Aurora-A. Most importantly this study revealed that Aurora-A binds to TPX2, a known component of the spindle apparatus. Binding studies demonstrated that the first 43 amino acids of …
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Mechanisms of Cytokine-Induced IGF -I Insensitivity in Cancer Cells
… the S phase of the cell cycle, leading to G1 arrest in MCF-7 cells. TNFalpha and IL-1beta act by inhibiting the ability of IGF-I to tyrosine phosphorylate insulin receptor substrate-1 (IRS-1), to induce accumulation of both E2F-1 and cyclin A, to hyperphosphorylate retinoblastoma (RB), and …
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Increased Rate and Extent of the P53 Dna Damage Response During S-Phase of the Cell Cycle
… apoptotic response), p21 (mostly involved in G1 arrest), and MDM2 (inhibitor of p53). Under stress conditions, p53 must be activated to avoid replication of cells containing damaged DNA. However, in healthy cells, when cell cycle arrest or apoptosis should not be induced, p53 must be …
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The Impact of Clinically Relevant Culture on Human Haematopoietic Stem Cells: A Kinetic Analysis at Single Cell Resolution
… reduced survival, cell cycle progression (late G1-M), sharp upregulation of MYC and a reduced ability to resolve proteostatic stress. Taken together, my results demonstrate that the 6 to 24 hr transition point is instrumental for the determination of LT-HSC cell fate ex vivo. Finally, using an …
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The candidate tumour suppressor, XIAP associated factor 1 (XAF1), directly inhibits XIAP activity and induces G1 phase cell cycle arrest
… of XAF1A in HEL299 cells triggered a G1 cell cycle arrest. This G1 arrest coincides with an increase in p21, but not p53. The ability of XAF1 to block XIAP function and induce cell cycle arrest suggests a role for XAF1 in the control of both apoptosis and cell growth. The coding …
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Ruolo dell'Enantiomero (R)-9-HSA nel controllo della proliferazione in una linea di Adenocarcinoma del Colon umano
… cell line, induced a proliferative arrest in G0/G1 phase mediated by a direct activation of the p21WAF1 gene, bypassing p53. We have previously shown that 9-HSA controls cell growth and differentiation by inhibiting histone deacetylase 1 (HDAC1) activity, showing interesting features …
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1á,25(OH)2-VITAMIN D3 IN PROSTATE: INTERSECTION WITH AKT/PTEN AXIS AND ROLE IN SENESCENCE
… and an AKT inhibitor cooperated to induce G1 arrest, senescence and higher p21 levels in prostate cancer cells. As AKT activation in prostate cancer usually occurs due to the loss of tumor suppressor PTEN, we evaluated the effect of Pten loss on 1á,25(OH)<sub>2</sub>D<sub>3</sub>)-mediated …
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hCLCA2 IS A p53-REGULATED GENE REQUIRED FOR MESENCHYMAL TO EPITHELIAL TRANSITION IN BREAST
… In cell culture, conditions that cause G1 arrest such as contact inhibition and depriving cells of growth factors and anchorage induced these genes. Therefore, one of the goals of this project was to find if this is true of hCLCA2 in human breast epithelial cells. We found that hCLCA2 …
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Investigation of T cell signalling events regulating immunity and tolerance in vivo
… of the anergic state, as indicated by G1 arrest, and hence, one or more additional negative signals may be required. Indeed, this study suggests that perhaps such a negative signal could be provided by the downregulation of p-Rb and/or increased expression of inactive cyclin dependent …