Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 107 for “"G-proteins"”.
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The Cloning and Characterization of Two ROP/RAC G-Proteins from Gossypium Hirsutum
Rop/Rac proteins are plant-specific monomeric guanosine triphosphate-binding proteins (G-proteins) with important functions in plant development. Until recently, only three cotton (Gossypium hirsutum) Rop/Rac G-protein genes were sequenced, representing subfamilies III and IV of the plant monomeric …
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Abnormalities in Post-Translational Processing of Platelet Rap 1B in NIDDM: A Possible Cause of Platelet Hyperactivity and Cardiovascular Disease in Diabetes
… localization and function of platelet G-proteins. The majority of the platelet responses to agonists are mediated through specific receptor/G-protein complexes. Therefore, G-protein activity is central to "normal" platelet activity (i.e. aggregation). We have shown that Simvastatin, the …
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Exploring the molecular signatures of the ligand-receptor-Gi/o protein interactions of adenosine A1 and A3 receptors
… at the level of the activation of individual G proteins. Being primarily coupled to the inhibitory G proteins, hA1R/hA3R showed little activation at the non-inhibitory G proteins (Gss, GsL, Gq, G11, G15, G12 and G13). It has been discovered in this part of the thesis that hA1R/hA3R showed …
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Novel interactions of the 5-HT₂ₐ and related receptors with intracellular signalling proteins
… interact with and signal through the trimeric G-proteins. The 5-hydroxytryptamine 2A receptor (5- HT₂ₐR) is a member ofthe group I family of rhodopsin-related GPCRs. The receptor is known to activate phospholipase C (PLC) via the heterotrimeric G proteins Gaq/n, but has been shown to also signal …
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The G protein-coupling specificity of D2-like dopamine receptors
… mutants of individual Gi-family G proteins could be expressed in an inducible fashion. A range of ligands displayed agonism at the long isoform of the human dopamine D2 receptor. However, varying degrees of efficacy were observed for individual ligands as monitored by their capacity …
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The role of eicoapentaenoic acid in cancer cahexia
… an interaction with guanine nucleotide binding proteins (G-proteins). Progression of the cachectic state induced an up-regulation of adipocyte membrane expression of stimulatory G-proteins, allied with a concomitant down-regulation of inhibitory G-proteins, thus facilitating the catabolic …
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Contribution of the Membrane-Proximal Region of the Vesicular Stomatitis Virus Glycoprotein to Host Cell Entry and Membrane Fusion
… deleted or mutated to alanines. The mutated G proteins were examined through the use of transient transfection assays for their surface expression levels, fusion capacity, and ability to undergo pH dependent conformational changes. Mutation of a conserved HPH motif (H423/P424/H425) resulted in …
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Localization of Goα in the Developing and Adult Mouse Brain
Heterotrimeric G proteins mediate signal transduction generated by hormone and neurotransmitters. Among G proteins, Go, a member of the Gi/o family, is the most abundant heterotrimeric G protein in the brain but the roles and effectors for the Go have not been clearly defined. As previously …
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Small gtp-binding proteins in insulin secretion
… GSIS involves interplay between small G-proteins and their regulatory factors. Herein, I tested the hypothesis that Arf nucleotide binding site opener [ARNO], a guanine nucleotide exchange factor [GEF] for the small G-protein Arf6, mediates the activation of Arf6, and that ARNO/Arf6 …
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Anti-allergic drug and intracellular signaling mechanisms in human blood eosinophils and airway epithelial cells,
… and airway epithelial cells involving G-proteins and decrease in intracellular free calcium.|The effect of IPD on G-proteins, activated nuclear factor kB (NF-kB), P (I) 3-kinase and Akt was evaluated using Western blotting on cellular lysates of HBECs and eosinophils treated with IPD. …
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Energy utilization of toad retina: A hydrogen-1 and phosphorus-31 NMR study
… and second messengers (Ca$\sp{2+}$ and G-proteins) are generalizable to many other biological systems.
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Experiments concerning the mechanism of cytokinesis in Caenorhabditis elegans embryos
… The furrow localization of LET-99 depended on G proteins, and consistent with this finding, G proteins are also required for aster-positioned cytokinesis. (Anlage: Quick time movies, 466, 67 MB)
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Role of Chaperonin CCT in G-protein Biosynthesis
… and function to assist the folding of other proteins. All chaperonins consist of two stacked rings, which come together to form a central cavity where folding can take place. The eukaryotic cytosolic chaperonin containing TCP1 (CCT) is a member of the group II chaperonins, which are defined …
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Evaluating luteinizing hormone receptor signaling using the cyclic AMP reporter ICUE3
… of LH receptors and subsequent activation of G proteins. Evaluating the cyclic adenosine monophosphate (cAMP) level in response to binding of ligand to LH receptors depends on activation of adenylyl cyclase by G proteins and production of cAMP. In this project, fluorescence resonance energy …
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Adenylyl Cyclase Type 9: Regulation and Cardiac Function
… contractile machinery, and stress response proteins that enhance cardiac function. Alterations of cAMP signaling occur in the failing heart and contribute to impaired function. Of the AC isoforms present in adult cardiomyocytes (AC 4, 5, 6, and 9), AC9 is the most divergent in sequence and …
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Biophysical Characterization of Tryptophan Locales, Mg²+ Binding and Protein Folding in Gα Subunits
… structure of guanine nucleotide - binding (G) proteins using a variety of spectroscopic tools. G proteins are membrane-bound proteins consisting of α, β, and γ subunits required for the transduction of extracellular signals to various intracellular effectors. Activation of G protein coupled …
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Biochemical and Biophysical Studies of Novel Features of Ras-related Protein Interactions
<p>The Ras superfamily of G-proteins are of great research interest for structure-function relationships among proteins as they act as molecular switches in the regulation of various biochemical reactions in the cell. They are regulated by protein-protein interactions targeted to the highly …
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Characterising the conformational dynamics of β₁-adrenergic receptor activation and G protein coupling
… (GPCR) family of seven-transmembrane signalling proteins facilitates cellular responses to environmental stimuli. GPCRs are integral to numerous physiological signalling pathways and thus many therapeutics have been developed to modulate receptor activity. Stimulation by agonist binding into an …
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Functionally selective receptor signaling and endocytosis by dopamine D1 receptor agonists
… receptor that signals through Gs and Golf G proteins. D1R agonists increase cAMP production and recruit β-arrestin to the plasma membrane, which may lead to receptor desensitization and/or induce D1R endocytosis. In addition, the D1R is essential for voluntary movement, cognition and reward …
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Adiponectin : a novel circulating anti-thrombotic factor in humans?
… coupled receptors linked to Gi-coupled G-proteins. Furthermore, the stimulation of Gi/Gz coupled G-proteins can potentiate platelet aggregation by stimulating secretion through a PKC-independent manner.Since adiponectin accumulates at sites of vascular lesions, the ability of immobilised …
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