Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Gαq"”.
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IS BRAIN CHOLESTEROL THE MISSING CULPRIT IN DRUG ADDICTION?
… found that more Gα subunits (Gαi1/2/3, Gαo and Gαq) were translocated into lipid rafts whereas their effectors such as adenylyl cyclase (AC) and phospholipase Cβ (PLCβ) remained in non-lipid rafts. Importantly, translocation of Gα subunit isoforms between lipid rafts and non-lipid rafts has …
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Signal Transduction Mechanisms Mediating the Regulation of Vascular G Protein-Coupled Receptors
… resistance and this is likely due to the reduced Gαq/11-coupled receptor desensitization. The vasodilatation in response to GαS-coupled receptor stimulation was also enhanced, but the increases in vasoconstrictor mechanisms dominate the physiological phenotype. In addition, VSMCs cultured from …
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Investigation of the function, pharmacology and oligomerisation of GPR40, GPR41 and GPR43
… chain fatty acids to be determined. A GPR40-Gαq fusion protein was also generated, but basal levels of [35S]GTPgammaS binding in Gαq immunoprecipitates was high and did not increase significantly upon the addition of long chain fatty acids. Treatment of membranes expressing GPR40-Gαq with …
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Influence of Peptide Allosteric Modulators on Agonist Bias at Class B1 G Protein-Coupled Receptors
… desensitisation was measured, with increases in Gαq coupling and intracellular Ca2+ (Ca2+)i mobilisation mediated by GLP1R transiently expressed in HEK293T cells. (Ca2+)I mobilisation was also increased by RAMP3 overexpression in INS-1 832/3 cells, which endogenously express the receptor. …
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Evaluation of Adipokinetic Hormone Receptor Function Suggests a Single Tissue is Responsible for Starvation Phenotypes in Drosophila.
… phenotypes. The AKHR can couple to either Gαs or Gαq in heterologous expression systems, but this study is the first example of quantification of AKHR signaling in vivo. Injection of AKH into intact animals causes calcium levels within the fat body to rise, and this response is absent in flies …
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Therapeutic Approaches to Insulin Resistance and Type 2 Diabetes
… a constitutively active receptor, which couples Gαq type G proteins leading to the activation of the MAP kinases. Overexpression of GPR21 markedly attenuated insulin signalling and promoted macrophage migration. Interestingly, the effect of GPR21 on insulin signalling lessened in the presence of …
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An Account of Chemical and Mechanical Regulation of TRPC6 Channels in Podocytes
… of TRPC6 activity, and this is mediated by the Gαq /PLC pathway. This angiotensin-evoked upregulation is partially dependent on the formation of reactive oxygen species (ROS). In mouse podocyte cell lines, insulin causes upregulation of TRPC6 through increasing ROS formation via NADPH oxidase 4 …
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MRGPRX2 Signaling on Mast Cell-Mediated Pseudoallergy and Neuroimmune Interaction
… mobilization and degranulation via both Gαi and Gαq. Next, we utilized information obtained from both structural modeling and naturally occurring MRGPRX2 missense variants to identify putative G protein coupling regions. In addition, several gain- and loss-of-function missense single nucleotide …
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Investigating the Role of the IP₃ Signalling Pathway in RNA Interference in C. elegans
… mechanism. EGL-8 is canonically activated by Gαq homologue ELG-30. However, by screening the RNAi responses of Gα signalling mutants using a range of assays, EGL-30 was ruled out as an activator of EGL-8 in the pathway regulating RNAi, since EGL-30 loss of function does not result in an …
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The role of the kisspeptin receptor, KISS1R, in breast cancer cell proliferation, invasion and migration.
… coupled receptor that couples through the Gαq/11 pathway. It has been shown that KISS1R activation by kisspeptin results in the activation of phospholipase C, calcium mobilization and ERK1/2 phosphorylation. Moreover, kisspeptin production has been linked to inhibition of metastasis in many …
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Identification of a Dual-Action Small Molecule with Potent Anti-diabetic and Anti-obesity Activity
… from L-cells was blocked by YM-254890, a Gαq inhibitor. Consistent with our in vitro study, a single dose of EA acutely stimulated GLP-1 secretion in mice, accompanied with an improved oral glucose tolerance. Chronic administration of EA restored the impaired glucose and lipid homeostasis …
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Dissection of GnRH receptor-G protein coupling
… receptor to interact with Gq/11, Gi/o and Gs in Gαq/11 knockout MEF cells. My results indicate that the receptor is unable to interact with Gi/o but can signal through Gq/11. Additionally, my data do not support the suggestion of GnRH receptor-Gs interaction. Furthermore, I show that the GnRH …
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Studies on the mode of action of dermonecrotic toxins: Effects on adipogenic signaling pathways and delivery of c-terminal cargo into the cytosol
… of Notch1 does not occur through Rho/ROCK or Gαq but through G12/13 signaling. We present herein a better-defined trafficking pathway of PMT and mechanism by which the toxin delivers the activity domain into host cell. Using an HA-tagged PMT (PMT-HA), we show the subcellular localization and …
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ROLE OF LYSOPHOSPHATIDIC ACID IN REGULATION OF CANCER CELL METABOLISM
… Gα12-Rho-Rock pathway to activate SREBPs and the Gαq-PLC (phospholipase C) pathway to inactivate AMPK. Consistent with LPA mediated activation of the key lipogenic enzymes FAS and ACC, LPA stimulated de novo lipid synthesis via LPA2, leading to accumulation of intracellular triacylglycerol and …
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GPCR to Channel Communication in a Membrane Signaling Pathway
… to the Gαs and Gαi pathways, there is also the Gαq signaling pathway. In this pathway, activation of Gq-coupled GPCRs can lead to the activation of phospholipase Cβ (PLCβ). This enzyme can then hydrolyze phosphatidylinositol 4,5- bisphosphate (PI(4,5)P<sub>2</sub>) and thus decrease inward …
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A STICKY SITUATION: ESSENTIAL ROLES OF ADHESION G PROTEIN COUPLED RECEPTORS ADGRF5 AND ADGRG1 IN CARDIAC HOMEOSTASIS AND FAILURE
… system, we identified that ADGRF5 couples with Gαq/11-mediated signaling in healthy myocytes and with the use of a Gq/11 inhibitor, expression of these genes is restored, suggesting that ADGRF5 intracellular signaling contributes to their expression. Further mechanistic studies using Givinostat, …