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Showing 1 to 20 of 49 for “"Fura 2"”.
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Intravenous anaesthetics inhibit nicotinic receptor-induced membrane currents and Ca 2+ -transients in rat intracardiac neurons
… in neonatal rat intracardiac neurons using fura-2 photometry and patch-clamp recordings. Extensive preliminary tests using fura-2 containing solutions in the presence or absence of the anaesthetics were carried out to detect any interference of these drugs with the fura-2 fluorescence …
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Die pH-Regulation isoliert in vitro perfundierter Kolonkrypten der Ratte
… Experimenten durch parallele Perfusion von fura-2 kontrolliert: Der luminale Lösungswechsel konnte dann über die Aufzeichnung der fura-2 spezifischen Fluoreszenz simultan zum BCECF-Signal verfolgt werden. Luminale Perfusion von CO2/HCO3- und Essigsäure/Acetat bewirkte eine Azidifizierung, …
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Characterization of the Biological Functions of Human Recombinant Zona Pellucida Protein 3
… potentiation study of progesterone and rhZP3 by FURA-2 spectrofluorometry.</p> <p>Hence the present study concludes that rhZP3 is both a binding ligand for the sperm-zona pellucida interaction and acrosome reaction inducer. It is deduced that the optimal experimental conditions of the rhZP3 in …
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Charakterisierung von purinergen Rezeptoren an kultivierten Podozyten der Maus
… Technik und intrazellulärer Ca2+-Messung (Fura 2) beobachtet, die eine Depolarisation und einen Anstieg der Ca2+-Konzentration auslösten. In der RT-PCR wurde die mRNA von P2Y1, P2Y2, P2Y6, und P2X7 Rezeproren nachgewiesen. <br>Die funktionelle Aktivität der Rezeptoren P2Y2 und P2Y6 konnte …
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Die Bindung von Ca2+-Ionen an Zebrafisch-GCAP4
… Ca2+-Bindungs-Assay mit dem Fluoreszenzfarbstoff Fura-2 als Ca2+-Indikator etabliert, der für alle zGCAP4-Varianten (und auch für myrbGCAP2) die Bindung von 3 Ca2+-Ionen ergab. Allerdings scheint es, dass tatsächlich nur 2 Ca2+-Ionen titriert wurden und dass dritte lediglich auf die Insensitivtät …
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Mechanismen der Ciclosporin A - induzierten Nephrotoxizität : eine Untersuchung an proximalen Tubuluszellen
… Tubuluszellen. <br>Untersuchungen mittels Fura-2-Mikrospektrofluorometrie zeigten, dass CyA zu einem genuinen, dosisabhängigen [Ca2+]i-Anstieg in LLC-PK1-Zellen führt. Diesem [Ca2+]i-Anstieg liegen mindestens drei Ursachen zugrunde: <br>(1) Es kommt zu einem Calcium-Einstrom über …
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Novel magnesium influx pathways in isolated renal thick ascending limb cells
… determined using magnesium fluorescent dye, mag-fura-2 and the measurement of magnesium refill rates (d[Me] jdt) was used as a means to characterize the magnesium pathway in the plasma membrane. Isolated cTAL cells were pretreated with Me-free media for 16-24 hours and d[Me];/dt was tested in …
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Magnesium Regulation of Calcium in Essential Hypertension
… cells (MNC) loaded with the calcium indicator fura-2 AM. Plasma and RBC total magnesium were measured and found to be considerably lower in magnesium-deficient rats. Magnesium-deficient RBC of both SHR and WKY demonstrated lower Ca ATPase activity when stimulated either with 0.3 μΜ or 35 μΜ …
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Lead Activation of a Developmentally Regulated Calcium Channel in Rat Hippocampal Nerve Terminals
… the intracellular (Ca<sup>2+</sup>) indicator Fura-2. The changes in cytoplasmic free calcium ([Ca<sup>2+</sup>]<sub>i</sub>) were measured by stopped-flow fluorescence spectroscopy following depolarization with elevated potassium on a millisecond time scale (Lentzner et al., 1992). …
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Mechanism and role of calcium in excitation-mucus secretion coupling in human colonic epithelium
… the muscarinic receptor (M3AchR) was expressed. Fura-2 Ca2+ imaging demonstrated that Carbachol (Cch) evoked Ca2+ signals at the human colonic crypt base via M3AchR activation, which then spread to all cell types along the crypt-axis. Surprisingly, pharmacological inhibition of the canonical 2nd …
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S-Acylation Is A Key Regulator of Orai1/Stim1-Mediated Store-Operated Calcium Entry In T Cells
… Furthermore, using electrophysiology studies and Fura-2 Ca<sup>2+</sup> imaging, we demonstrated that both Orai1 and STIM1 require S-acylation to form active CRAC channels and mediate SOCE. Lastly, our studies using total internal reflection fluorescence microscopy showed that a lack of …
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Ryanodine receptor expression in trophoblasts
… of various RyR agonists and antagonists, using fura-2 fluorescent videomicroscopy. Furthermore, endogenous placental peptide hormones, namely angiotensin II, arginine vasopressin and endothelin 1, were demonstrated to increase [Ca2+]i in BeWo cells, and such increases were suppressed by RyR …
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Changes in Skeletal Muscle Sarcoplasmic Reticulum Function in Adult and Aged Fisher 344 Brown x Norway Rats
… group 27 months. A Jasco CAF-110 Fluorometer and fura-2 were used to determine the rate of Ca2+ uptake and release by isolated SR vesicles. In the aged animals, mass of the soleus was reduced by 22%, while the plantaris was reduced by 23%, and the diaphragm by 15%. However, these differences were …
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Ryanodine receptors in calcium signaling pathways
… induced by RyR activators or regulators, using fura-2 AM as the Ca<sup>2+</sup> indicator. Successful RyR<sub>2</sub> gene knockdown allowed us to carry out some initial experiments to characterize the specific roles played by the RyR<sub>2</sub> receptor isoform. We examined cell responses to …
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Calcium, actin and the spectrin skeleton: Responses to swelling and shrinking perturbations.
… in swelling molluscan neurons was measured by FURA-2 ratiometric microscopy using perturbations which induced vacuole-like dilations (VLDs). (Ca) $\rm\sb{int}$ was measured in either normal (3.5mM) or low (0.5$\mu$M) extracellular calcium. The small but statistically significant changes …
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New optical techniques and hardware for studying live cell dynamics
… and demonstrate its application for ratiometric Fura-2 Ca2+ imaging of live cell specimens with sub-5 nM precision that supports full frame video-rate temporal resolutions.
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The Role of Protein S-glutathionylation on Ca2+ Signaling in Cultured Aortic Endothelial Cells
… level using the fluorescent Ca2+ indicator, fura-2 and time-lapse video microscopy. Low concentrations (50, 100 ¿¿M) of diamide reversibly increased spontaneous Ca2+ oscillations, whereas high concentrations (250, 500 ¿¿M) of diamide caused an immediate, synchronized Ca2+ oscillation in …
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Postnatal Maturation Modulates Cerebrovascular Myogenic Tone
… simultaneously in isolated endothelium denuded, fura-2 loaded middle cerebral arteries (MCA) from pup (PI4) and adult (6 months old) SD rats. We studied the mechanism that govern myogenic tone and examined the hypothesis that during maturation, MCA from rat pups are more reliant on calcium influx …
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Etablierung eines Expressions- und Testsystems für Membranrezeptoren an Oozyten von Xenopus laevis
… und dem Ca2+-Indikatorfarbstoff Fura-2 gemessen wurde. Dabei konnten innerhalb der ersten Minuten nach PROG-Zugabe keine Veränderungen von [Ca2+]i gefunden werden. Lysophosphatidylsäure (LPA) hingegen löste sehr zuverlässig Calcium-Signale aus. Durch Thrombin, Angiotensin II und …
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Coronary artery disease in metabolic syndrome: a role for the sarcoplasmic reticulum Ca2+ ATPase
… In particular, we used the fluorescent Ca2+ dye, fura-2, to examine alterations in CSM intracellular Ca2+ regulation during CAD progression, as perturbations in intracellular Ca2+ regulation are implicated in several cellular processes associated with CAD pathology, including CSM contractile …
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