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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Functional metagenomics"”.
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The Application of Functional Metagenomics to Natural Products Research
… currently in use (collectively termed "metagenomics"), the strategies of functional metagenomics provide access to important functions, phenotypes and activities encoded on fragments of environmental DNA (eDNA) as evidenced by the expression of these traits in model cultured bacterial …
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Combining microbial functional metagenomics and Caenorhabditis elegans genetics to uncover and characterise novel bioactive compounds
… first aim of this thesis was to develop a novel functional (meta) genomic screen for the identification of inhibitors that target the nematode Caenorhabditis elegans. Environmental DNA from marine habitats was expressed in Escherichia coli and the resulting functional (meta) genomic libraries …
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Towards in vivo editing of the human microbiome
… In particular, we developed a temporal functional metagenomics method to identify genes that improved microbial fitness in the mammalian gut in vivo. We also built foundational tools for delivering genetic elements and immunizing endogenous microbiota against acquiring antibiotic …
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Identification of Quorum Sensing Genes in Hypersaline Microbial Mats
… social units and ultimately define the overall functionality of ecosystems. Bacterial communities in all ecosystems likely utilize signaling pathways to adapt to localized changes in environmental conditions. One such pathway is called quorum sensing (QS), a cell-density dependent phenomenon …
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Ultrahigh-Throughput Microfluidic Droplet Screening of Metagenomic Libraries for Esterases and Kemp Eliminases
In the search for new enzymes, functional metagenomic libraries are particularly interesting since they give access to the genomes of the 99% of microorganisms which cannot be cultured in the laboratory. However, using classical biochemical assays to screen such libraries is impeded by the fact …
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Functional metagenomic discovery and characterisation of CAZymes by microfluidic methods
… to the small number of experimentally verified functional annotation, which is crucial for the accurate assignment of enzyme activities. Functional metagenomics provides an alternative to the sequence-based exploration of the protein landscape. Experimental screening for new enzymes based on …