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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Functional DNA"”.
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Functional DNA Aptamers as Biotherapeutic Molecules
Aptamers are single-stranded oligonucleotides, DNA or RNA, which can bind to a myriad of targets such as ions, peptides, proteins, drugs, organic and inorganic molecules with high affinity and specificity. Aptamers are derived using combinatorial libraries comprised of a variable region flanked by …
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Functional dna: Biochemical/biophysical characterization & sensing applications
… targets. In 1994 the Joyce group showed that DNA, long thought to be solely for information storage, was capable of catalysis. Naturally occurring ribozymes were discovered in the 1980s by the Cech group. Since then aptamers, which bind targets, and ribozymes or deoxyribozymes, which are …
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Bioinspired functional DNA-nanomaterial conjugates for diagnostic and therapeutic applications
… at the nanoscale regime, but also produces novel functional materials with promising applications. Nanomaterials with size similar to biological molecules and systems have shown great promise for diagnostic and therapeutic applications due to their unique properties, such as fluorescent, …
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Development of Functional DNA -Based Sensors and Investigations Into Their Mechanism
… point towards the development of a successful functional nucleic acid-based sensor is its isolation and this is done through in vitro selection. In vitro selection to isolate DNAzymes for Hg2+ and Cd2+ and the use of negative selections to overcome Pb 2+ interference at various stages of …
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Development of functional DNA-based sensors and investigations into their mechanism
The discovery that nucleic acids could perform functional roles in addition to being genetic materials carriers opened doors to a new paradigm in nucleic acid chemistry. Catalytic DNA molecules also known as deoxyribozymes or DNAzymes were first isolated in 1994 through an in vitro selection …
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Discovery of novel functional DNA and their application as biological sensors
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-05-01
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Functional DNA repair capacity assays : a focus on base excision repair
The integrity of our DNA is challenged by roughly 100,000 lesions per cell on a daily basis. Failure to cope with DNA damage can lead to cancer, immunodeficiency and degenerative disease. Quantitating and understanding an individual's DNA repair capacity may enable us to predict and prevent disease …
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Novel MRI Contrast Agents Based on Functional DNA and Nanomaterial Conjugates for Biomedical Applications
Finally, we have demonstrated that the fluorescein encapsulated one micron liposomes can be used to detect inorganic mercury in water. At the same time, we have shown that chelators of inorganic mercury can be encapsulated in liposomes and released upon mercury detection.
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Novel methods for on-site and cost-effective quantification of multiple targets using functional DNA and portable instruments
… quantitative assays of multiple targets using functional DNA and portable instruments. Such assay methods are important for personal care as well as analyte detection processes. Recent advancements such as the personal glucose meter method developed can achieve fast but not low-cost or …
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Advances in DNA technology towards dynamic assemblies and biological applications
… published his seminal work on the design of DNA tiles, which along with his group, became the field now known as structural DNA nanotechnology. Professor Chad Mirkin and several others then later realized the power of complementary DNA can be utilized as a “smart glue” to programmably …
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Bioinspired fabrication and assembly of hybrid materials for sensing and biomedical applications
… transduction. In this document, several new functional hybrid materials or devices are demonstrated to tackle the above-mentioned challenges by integrating biomolecules with nanomaterials. Development of colorimetric and fluorescent biosensors for heavy metal ion detection based on functional …
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DNA as a functional molecule for control of nanomaterial morphology and for biosensing
DNA, traditionally known as the carrier of genetic information has proven that it can be utilized as a key player for self-assembly, material synthesis and molecular sensors. Given the ease of synthesis and modification, precise control over sequence and specificity in base pairing, the number of …
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In vitro selection and characterization of mono-, di-, and trivalent metal-dependent DNAzymes and their sensing applications
… acid enzymes and aptamers that can perform functional roles other than storing genetic information; a new paradigm in nucleic acid chemistry has been opened. In 1990 the first artificially isolated functional nucleic acid was discovered through an in vitro process called SELEX, Systematic …
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DNA-based Artificial Mimics of Cell-surface Machinery
… of cells has evolved to mediate a broad array of functionalities critical to life, such as molecular trafficking, signal transduction, motility, adhesion and communication. These functionalities are often reliant on highly sophisticated membrane-anchored nano-machines, and enabled by the ability …
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Probing PAX6-DNA interactions using high-throughput yeast one-hybrid assays and deep mutational scanning
… pathologies. The interplay between PAX6 and its DNA targets is complicated by multiple functionally distinct subdomains, co-factors, and divergent spectra of disease phenotypes. Knowledge of the contributions made to binding by each residue and the impact of missense variants is likely key for …
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The role of DNA sequence during helicase loading at S. cerevisiae origins of replication
Eukaryotic chromosomal DNA replication is a tightly regulated process that initiates at multiple origins of replication throughout the genome. As cells enter the GI phase of the cell cycle, the Mcm2-7 replicative helicase is loaded at all potential origins of replication but remains inactive until …
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DNA Three-way Junctions as Scaffolds for Hybrid Catalysis
… new approaches to catalysis is essential. Bio-functionalised DNA is known to catalyse a number of industrially important chemical reactions, and DNA supported catalysts have the advantage of being biodegradable and non-toxic, and are therefore of interest as green alternatives to traditional …
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Molecular mechanisms of phenotypic plasticity: Explorations of DNA methylation and transcriptional regulation using the honey bee
… important aspects of transcriptional regulation: DNA methylation and transcription regulation by a nuclear receptor, Ultraspiracle (USP). Chapter 2 presents results that demonstrate that there is a functional CpG methylation system in honey bees, the first well-characterized functional DNA …
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Cloning and characterization of a single-stranded DNA-binding protein of Leishmania major
… (CTCGCC )represented a potential site of protein-DNA interaction, a A. gtl 1 expression library ofL. major was screened with a radio labelled oligonucleotide probe to detect clones expressing functional DNA-binding proteins. A gene was isolated which encoded a novel DNA-binding protein, referred …
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Investigating Genetic (IN)Compatibility Between Temperate Phages and CRISPR-CAS Systems in Staphylococcus Aureus
… directed at a particular breed of bacterial DNA viruses, known as temperate phages, which cause both harmful (lytic) and benign (lysogenic) infections under different conditions. Initial studies investigating prokaryotic CRISPR-Cas immunity seemed to indicate that functional, DNA-targeting …
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