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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

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Showing 1 to 20 of 143 for “"Functional Assays"”.

  1. Single-cell approaches reveal functional and molecular heterogeneity in malignant haematopoietic stem cells

    Shepherd, MairiUniversity of Cambridge2020

    … study utilises single-cell gene expression and functional assays to identify a subset of JAK2 V617F mutant HSCs that display defective self-renewal. This defect is rescued by crossing JAK2 V617F mice with mice lacking TET2, the most commonly co-mutated gene in patients with MPN. Single-cell gene …

    cambridge Repository record

  2. Functional and Transcriptional Heterogeneity of the Human Haematopoietic Stem Cell Pool at Steady-State and Under Inflammation

    Calderbank, EmilyUniversity of Cambridge2021

    Blood production is coordinated by a functionally heterogeneous pool of multipotent haematopoietic stem cells (HSCs), downstream of which lineage-restricted progenitors are generated. The advent of single cell technologies has changed our view of the haematopoiesis to a dynamic continuum. …

    cambridge Repository record

  3. Systematically and proactively testing variant effects for AIRE and SOD1

    Axakova, Anna2024

    … variant annotation comes from (often cell-based) functional assays of variant impacts. One-at-a-time variant functional assays are the current standard, where results often lag months or years after a rare variant has first been detected in a patient. Variant effect ‘maps’ are ‘look-up tables’ …

    toronto-retro Repository record

  4. Development of a Novel 3-Dimensional Testicular Organoid Model for the Study of Human Spermatogenesis and Gonadotoxicity In Vitro

    Pendergraft, Samuel SparrowWake Forest University2016

    … data. However, current gold standard in vivo functional assays in animals are limited in their human predictive capacity, and existing human in vitro models of testicular toxicity do not recapitulate the complex signaling interactions of the native tissue environment. The overall goal of this …

    wfu Repository record

  5. 2D layout of bead based thioaptamer/aptamer selection platform for therapeutics and diagnostics

    Xu ZhaoThe University of Texas Medical Branch2007

    … the 2D layout of beads and its corresponding functional assays, a model system is first established: NF-kappa B proteins were expressed, purified and characterized. Thioaptamer XBY6, which specifically targets NF-kappa B protein, and its natural origin, I-kappa B were synthesized and verified. …

    utmb Repository record

  6. Development and Characterization of Selective CB2R Inverse Agonists as a Novel Anti-Inflammatory Therapy for Neuroinflammation – Mechanisms of Action in Murine and Human Microglia Models Provide Valuable Insights into Their Therapeutic Potential

    Alghamdi, Sahar Saleh2021

    … and evaluated in ACTOne and TANGO-bla functional assays for CB1 and CB2 activity in combination with affinity measurements for the CB1/2 receptors. Moreover, the compounds that demonstrated no activity at functional assays with high binding affinities to CB2R were further assessed in …

    tenn-hsc Repository record

  7. In Vitro studies of factors which may influence ligand binding, function, immunogenicity and genetic regulation of the Beta-2 adrenergic receptor in asthma

    Potter, Paul CharlesDivision of Pulmonology1991

    … influence the expression, ligand binding and functional activity of the beta-2 adrenergic receptor. These studies were prompted by previous reports that the postulated beta-2 adrenergic receptor abnormality in allergic asthma could be induced, induced by autoantibodies. I established and …

    cape-town Repository record

  8. Searching for structural determinants of agonist selectivity in nicotinic acetylcholine receptors

    Mínguez Viñas, TeresaOxford Brookes University2020

    … aid the design of novel smoking cessation drugs. Functional and radioligand binding assays showed that novel C(10) cytisine (Cyt) derivatives maintain the potency and efficacy of the smoking cessation drug Cyt at α4β2 nAChR but had no significant activity at α7 subtype. Molecular docking in …

    oxford-brookes Repository record

  9. The human cannabinoid CB(1) receptor stably expressed in Chinese hamster ovary cells provides a model system to predict the pharmacological effects of cannabinoids in man

    Landsman, Robert ShawnThe University of Arizona.1999

    … the effects of cannabinoid ligands in these functional assays, confirming that the effects of these compounds are mediated through inhibitory Gᵢ/ₒ proteins. The data obtained from radioligand binding and functional assays with the human cannabinoid CB₁ receptor stably expressed in Chinese …

    arizona-thes Repository record

  10. FMF assay for assessing vaccine generated antibodies in a biomimetic manner

    Dhir, Vipra

    Traditional functional assays such as hemagglutination inhibition (HAI) and micro-neutralization (MN) assays have been routinely used for assessing the vaccine response, since influenza vaccine has been administered in people (1940). Such assays are not always predictive regarding the protection …

    ucf

  11. Identifying Novel Interaction Between the E3 Ubiquitin Ligase Ring Finger Protein 20 (RNF20) and the Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)

    Gill, Jatin2026

    … for USP7. Through various biochemical and functional assays, including endogenous co-immunoprecipitation, comparing parental and USP7 knockout HCT116 cells, reconstitution assay, overexpression with USP7 or the catalytic mutant USP7, cycloheximide chase and a deubiquitination assay, we show …

    york Repository record

  12. DEVELOPMENT AND UTILIZATION OF MICROFLUIDIC TRAP (TRYPSIN-BASED RECOVERY AFTER PERMEATION)

    ZHOU JIANXUAN2024

    … viability and integrity, enabling their use in functional assays and biochemical analyses such as western blot, qPCR, and flow cytometry. It provides valuable insights into cellular behaviors and responses as a function of confinement, enhancing our understanding of cell dynamics in confined …

    nus Repository record

  13. ¿ROLE OF ENDOTHELIAL DYSFUNCTION IN THE PATHOGENESIS OF UNPROVOKED VENOUS THROMBOEMBOLISM¿

    CANCELLARA, ASSUNTAUniversità degli Studi di Milano2023

    … an optimal non-invasive model to assess the functionality of the endothelial compartment. In particular, in this project we aimed to: • set-up and optimize ECFC-based functional assays aimed at assessing thrombogenic endothelial properties; • investigate the presence of constitutive ED in …

    milano Repository record

  14. A bioactive peptide QUB3009 from the defensive skin secretion of the frog, Phyllomedusa tomopterna

    Yuan, JialinQueen's University Belfast2017

    … The synthetic replicate was subjected to functional assays, including antimicrobial assays, haemolysis assays and anticancer cell assays. The peptide was demonstrated to have antimicrobial activity against S. aureus, E. coli and C. albicans at concentrations of 64, 64 and 32 µM, …

    qu-belfast Repository record

  15. Combined ultrasound, photoacoustic and elasticity microscope for tissue engineers

    Mallidi, SrivalleeshaThe University of Texas at Austin2006

    … Currently, the ability to have quantitative functional assays of engineered tissues is limited to existing invasive methods like biopsy. Hence, an imaging tool for non-invasive and simultaneous evaluation of the anatomical and functional properties of the engineered tissue is needed. In this …

    texas Repository record

  16. A bioactive peptide QUB2177 from the defensive skin secretion of the frog, <i>Odorrana livida</i>

    He, XinQueen's University Belfast2017

    … was evaluated for its biological effect through functional assays. The results showed that QUB-2177 possessed neither significant antimicrobial nor haemolytic activity on horse erythrocytes. Besides, similar negative results were also found in the assessments of anti-cancer activity, trypsin …

    qu-belfast Repository record

  17. The Investigation of the SKA3 Circular RNA Role in the Ovarian Cancer Progression

    Eshaghi, Esra2022

    … tumors with low malignant potential (LMP). Using functional assays, we demonstrated that overexpression of circSKA3 promoted, while the silence of cirSKA3 suppressed, proliferation, migration, and invasion in OVAR3, and CAOV3 cells derived from HGSC. We found that silencing of circSKA3 resulted in …

    york Repository record

  18. QUB-1951: a bioactive peptide from the skin secretion of the Chinese piebald Odorous frog, Odorrana schmackeri

    Hu, JiayiQueen's University Belfast2018

    … sufficient peptides to carry out the bioactive assays, solid phase peptide synthesis (SPPS) was used to synthesize. The molecular weight was confirmed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and purified by reverse phase-high performance liquid …

    qu-belfast Repository record

  19. Evaluating Phosphatidylinositide as Lipid Biomarkers for Hyperglycemia-related Triple-negative Breast Cancer Aggressiveness

    Kile, Aaron2025

    … progression. To address this, we combined functional assays that measure key cancer traits, including cell migration, attachment to the extracellular matrix (ECM), and cell accumulation, with lipidomic analyses of cell membranes under normal and high-glucose conditions. Our findings …

    iupui Repository record

  20. Interrogating the molecular mechanism of signal transduction in the TGF-β receptor superfamily

    Belcher, EmmaUniversity of Cambridge2025

    … Here, I combine sequence conservation analysis, functional assays and structural approaches to try to build a picture of the molecular choreography of TGF-β signalling. These results lay the groundwork for future studies to unravel the molecular mechanisms of signal transduction in the TGF-β …

    cambridge Repository record

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