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Showing 1 to 20 of 42 for “"FtsZ"”.
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Synthesis of FtsZ inhibitors : potential antibiotic agents
… one such novel target. The cell division protein FtsZ, which is a highly conserved, essential and druggable protein, could be utilised as a target in this fight against antibiotic resistance. This thesis aimed to develop a set of novel antibacterial compounds that inhibit the function of FtsZ. The …
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FtsZ Protofilament Curvature is the Opposite of Tubulin Rings
<p>Bacterial tubulin homolog FtsZ assembles straight protofilaments (pfs) that form the scaffold of the cytokinetic Z ring. These pfs can adopt a curved conformation forming a miniring or spiral tube 24 nm in diameter. Tubulin pfs also have a curved conformation, forming 42 nm tubulin rings. We …
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INSIGHTS INTO POLYMERISATION OF FTSZ AND OTHER CYTOMOTIVE FILAMENTS
… known to form cytomotive filaments. The protein FtsZ, a homologue of eukaryotic tubulins, forms cytomotive filaments that are used in almost all bacteria and many archaea to organise cell division. Here I show using X-ray crystallography and electron cryomicroscopy (cryoEM) that FtsZ switches …
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Molecular characterization of the cell division gene ftsZ in Neisseria gonorrhoeae.
… of many cell division genes. The ftsZ gene is essential in E. coli as the gene product plays a critical role in the initiation and formation of the septum at the division site. In efforts to understand cell division at the molecular level in N. gonorrhoeae, the ftsZ gene has been …
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Regulation of the Tubulin Homolog FtsZ in <i>Escherichia coli</i>
<p><em>Escherichia coli</em> is a well-known pathogen, and importantly, a widely used model organism in all fields of biological sciences for cloning, protein purification, and as a model for Gram-negative bacterial species. And yet, researchers do not fully understand how this bacterium replicates …
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PopZ and FtsZ coordinate polar growth termination and cell division in Agrobacterium tumefaciens
… to dissect the contributions of PopZ and FtsZ to polar growth and cell division. Although PopZ is not required for polar growth, it is required for proper coordination of polar growth, chromosome segregation, and cell division. This PopZ-mediated coordination ensures that daughter cells …
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Structure-function analysis of ZapC, an FtsZ-ring stabilizer, in Escherichia coli cytokinesis
… of a cytokinetic ring (Z ring) formed by FtsZ, a tubulin-like GTPase, at midcell. Division also involves the formation of a multi-protein complex at midcell known as the divisome. Several divisome proteins promote the assembly/disassembly processes of FtsZ, thereby exercising …
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FtsK Regulates the Assembly of the Division Machinery in the FtsZ-less Chlamydia trachomatis
… cell division in most other bacteria, including FtsZ, the central coordinator of divisome assembly. In the absence of FtsZ, we show that divisome assembly in Ct is initiated by FtsK, a chromosomal translocase. Chlamydial FtsK forms discrete foci at the septum and at the base of the progenitor …
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DEVELOPMENT OF SMALL MOLECULES AS INHIBITORS OF FTSZ AND RNPA ACTING AS POTENTIAL ANTIMICROBIAL AGENTS
… considered to develop new potential inhibitors: FtsZ and RnpA. FtsZ is a 40 kDa protein with 40 -50% of conservation among all bacteria and archaea. This protein represents the main actor of the bacterial cell division cycle, the essential process that allows the parent cell to divide through …
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Characterization of the interaction between FtsZ-ring stabilizer ZapC and the conserved protease ClpXP in E. coli cytokinesis
… by polymers of the essential tubulin-homolog FtsZ. Polymerization of FtsZ is regulated by a number of proteins that bind FtsZ and regulate its assembly/disassembly mechanisms. Despite being extensively studied, the molecular nature of the diverse protein-protein interactions that regulate the …
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Prosthecobacter BtubAB form bacterial mini microtubules
The tubulin/FtsZ superfamily contains a large set of proteins that spans through all kingdoms of life, with αβ-tubulins being the eukaryotic representatives and FtsZ being the best studied prokaryotic homologue. It is believed that all tubulin/FtsZ-related proteins have evolved from a common …
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Genetically encoded division machinery for cell free synthetic biology
… compartment. A particular emphasis was placed on FtsZ, a protein that oligomerizes into a ring at the midcell and splits the cell into two. FtsZ does not contain a membrane interaction domain. In vivo, FtsZ interactions with the membrane are mediated by FtsA and ZipA. Therefore, the influence of …
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Molecular Mechanism By Which The Escherichia Coli Nucleoid Occlusion Factor, Slma, Keeps Cytokinesis In Check
… at the cell center and is orchestrated by the FtsZ protein. FtsZ self-assembles into linear protofilaments in a GTP dependent manner to form a cytoskeletal scaffold called the Z-ring. The Z-ring provides the framework for the assembly of the division apparatus and determines the site of …
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Uncovering the Roles and Evolved Sequence Grammar of Hypervariable Intrinsically Disordered Proteins in Bacterial Cell Division
… the ring is termed the Z-ring after the protein FtsZ, which is foundational to ring formation and is the bacterial homolog of tubulin. Like tubulin, FtsZ is an assembling GTPase, where GTP binding promotes the cooperative assembly into FtsZ polymers that laterally associate to form bundles. While …
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Characterization of ftsA conditional lethal mutants shows that FtsA is required at early and late stages of cell division in Streptococcus pneumoniae
… division site, together or immediately after FtsZ, where it is needed both to tether FtsZ to the membrane and also to recruit to midcell the other cell division proteins. In agreement with this, FtsA interacts, at least, with itself, FtsZ, ZapA and the septal PBP, FtsI. Despite the advances in …
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Mutations Within and Between Early Cell Division Proteins and Their Effects On Division Regulation In Escherichia Coli
… consists mainly of three essential proteins: FtsZ and its membrane tethers FtsA and ZipA. In this work, I aimed to understand the early regulation of division in E. coli by investigating the structure/function relationships of the proto-ring proteins, as well as their interactions with one …
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Biochemical and structural studies of the FtsZ:FtsA complex and polymerising abilities of the FtsA protein
… composed of polymers of the tubulin-like protein FtsZ. The Z-ring constricts and through interaction with other components engages in remodelling the cell wall and membranes in order to yield two daughter cells. During this process, FtsZ is known to interact with FtsA, which is an early component …
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Organization and Regulation of Proteins Required For Initiation of Cell Division In Escherichia Coli
… form the cell division machine, or “divisome.” FtsZ, a bacterial homolog of eukaryotic tubulin, forms a ring-like structure, the “Z ring,” at midcell and serves as a scaffold for divisome assembly and potential force generator during synthesis of the division septum. Both FtsA, a bacterial …
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The bacterial actin-like cell division protein FtsA forms antiparallel double filaments upon binding of FtsN
… separation. Filaments of the tubulin homologue FtsZ form the scaffold for divisome assembly in the cytoplasm, the Z ring. Actin like FtsA tethers FtsZ to the inner membrane and facilitates recruitment of downstream divisome components. The late divisome component FtsN is a bitopic membrane …
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Regulators of Bacterial Cell Division: Investigations of Min Oscillation and Ftsa Activity
… step in divisome formation is the assembly of FtsZ into a Z ring. The work presented in this dissertation focuses on two proteins that regulate the Z ring: MinD, which is a part of the Min system and important for positioning of the divisome; and FtsA, involved in early and late stages of …
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