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Showing 1 to 13 of 13 for “"Freeze-thawing"”.
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Protection Mechanisms of Excipients on Lactate Dehydrogenase during Freeze-Thawing and Lyophilization
… protein, lactate dehydrogenase (LDH), during freeze-thawing and lyophilization. Cryoprotectants are excipients that protect labile molecules during freezing. Some investigators believe that preferential exclusion is the predominant mechanism to explain the cryoprotection of labile proteins by …
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Freeze-Thawing of Polyester/E-Glass as an Evaluation of an ASTM International Test Standard Proposal
… with applications subjected to freezing and thawing. FRP encompass many characteristics that are beneficial to these sectors and also in resisting this exposure. ASTM International has yet not developed a standard to test FRP materials resistance to freeze-thaw (FT) cycling exposure. The …
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Effect of bioxcell and triladyl extenders and removal of seminal plasma of equilibrated and cryopreserved goat semen
… reduced (P < 0.05) following equilibration and freeze-thawing process, respectively on washed semen groups. Live and normal spermatozoa percentages were drastically reduced in Bioxcell® (5.2±4.9) and Triladyl® (6.9±8.6) washed semen groups, following freeze-thawing. There was a significantly …
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Studies on the frost resistance of natural stone
This work deals with the effects of freeze-thawing on the deterioration of some types of natural stone, especially stones frequently used in old buildings in Sweden. Laboratory studies were performed mainly on Gotland sandstone. This stone has been widely used in old buildings in the whole Baltic …
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Factors affecting preservation of liquid and frozen ram spermatozoa
… of ram spermatozoa to the stress of freeze-thawing. When ram spermatozoa were subjected to freeze-thaw stress, there was more variation among treatments reflected in maintenance of both intact acrosomes and motile life (Experiment 2, Table 9) than when unfrozen sperm were studied …
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A surimi-like material made from beef and pork and a novel protease in bovine and porcine skeletal muscle
… NaCl up to 3%. Gel formation was not affected by freeze-thawing, and the addition of known cryoprotectants did not show any effect on the gel forming ability.
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Through Vial Impedance Spectroscopy (TVIS): A novel process analytical technology for investigating the thawing of frozen cell suspensions.
… of time. However, many cell types die when they freeze because of intracellular ice formation causing cell disruption from within (Inc. gene and protein expression, etc.) and extracellular ice formation causing concentration of solutes, and osmotic shrinkage, with the potential to damage cell …
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Development and In-Vitro Evaluation of Long Circulating Liposomes for Targeted Delivery of Gemcitabine and Irinotecan in Pancreatic Ductal Adenocarcinoma
… chloride. This dispersion was subjected to freeze thawing and subsequent membrane extrusion. The liposomes were surface modified by incubating the liposomes with 5 mol% of PEG or poly(2-ethyl-2-oxazoline) conjugate and dialyzed against 5% glucose for 12 hours. The study found that the …
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The Requirement for Viable Platelets in the Coagulation of Heparinized Blood
… The non-viable platelets were prepared by freeze/thawing, and the abnormally functional platelets resulted from the addition of adenosine or adenosine 5'-diphosphate (ADP).</p> <p>The ACT’s of the heparinized samples containing treated platelets were considerably longer than the ACT’s …
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Assembly, characterization and evaluation of a 3rd generation nanoparticle based drug carrier for metastatic breast cancer treatment
… at a 1:1 ratio was formed during the repeated freeze-thawing process. Supported immunoliposomes with high homogeneity in size and structure were purified by sucrose density gradient centrifugation. Thirdly, the drug loading, triggered release, cell binding, cell uptake and cell toxicities of …
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Investigating cultivation and biorefinery process techniques on the microalgae Porphyridium aerugineum and Chromochloris zofingiensis
… method of mild, selective cell disruption was freeze thawing the centrifuged pellet. Subsequently, phycocyanin was obtained from the resuspended pellet via centrifugation or microfiltration. This allowed recovery of the intracellular and water-soluble compounds with little impurities. …
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Structure-property relations of porcine trabecular bone and micro-CT imaging of various materials
… Effects of preservation (fresh, and 1 and 5 year freeze durations) and micro-CT radiation are also investigated. Cylindrical porcine specimens were made from femoral head and divided into three groups depending on the freezing period. We conclude, using multiple regression, that porosity and …