Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Free flow electrophoresis"”.
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Enhancing Micro Free Flow Electrophoresis: Detection, Application, And Fabrication
Micro Free Flow Electrophoresis (µFFE) is a separation technique where analytes are moved through a planar separation channel via pressure driven flow. Analytes streams are deflected laterally within an electric field applied perpendicular to the flow and separated from one another based on …
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Isolation of hog renal brush border membrane vesicles with application to the study of cadmium nephrotoxicity
… using differential centrifugation followed by free-flow electrophoresis or sucrose density centrifugation. Utilizing the self-forming gradient capabilities of Percoll, we have developed a less cumbersome procedure to effect simultaneous isolation of brush border and basal-lateral membrane …
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On transient assemblies in amyloid formation: mechanistic insights and therapeutic strategies
… a novel microfluidic platform integrating free-flow electrophoresis with complementary analytical techniques, which allows for a multifaceted characterisation of amyloid oligomers. In this approach, heterogenous oligomer mixtures are fractionated on-chip and subsequently collected for …
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Development of Original and Notable Techniques for Protein Analysis, Native Identification, and Characterisation
… separating the particles using capillary electrophoresis and diffusional sizing. This allowed to characterise the components of heterogenous solutions in function of their size and the mobility of the proteins. The third chapter is looking at existing well-known microfluidic techniques, …
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Microfluidic Methods for Biomolecular Analysis
… from fluid behaviour on bulk scales and laminar flow is the key characteristic on this scale. Microfluidic systems have a wide range of applications in many disciplines from engineering to physics, chemistry and biotechnology. In this thesis, I explore different strategies exploiting the …
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Analytik und präparative Trennungen liposomaler Systeme mit Zonen-Free-Flow Elektrophorese
… herauszufinden, inwieweit sich die Methode der Free-Flow Elektrophorese (FFE) dazu eignet, liposomale Systeme aufzutrennen. <br>Zwischen einzelnen Liposomen findet ein Lipidaustausch statt, d.h. Lipide wechseln ihren Aufenthaltsort zwischen zwei benachbarten Liposomen. Die FFE ermöglicht auf …
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Downstream processing of recombinant and endogenous proteins from livestock milk
… chromatography(HIC), Recycle continuous flow electrophoresis(RCFE) and Free flow isoelectric focusing(FFIEF). The first process(EBAC/HIC) used with Zn2+ as a selective precipitating agent, purified recombinant human protein C(rhPC) and IgG(contaminated with less than 1% IgA) from swine …
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Herstellung und Charakterisierung von unsymmetrischen Liposomen unter Verwendung von rekombinanten Proteinen
… der beiden Liposomenpopulationen gelang über die Free Flow Elektrophorese (FFE). Bei der FFE handelt es sich um eine trägerfreie Elektrophorese, bei der Proben in einem laminaren Pufferstrom aufgetrennt und in 96 Fraktionen gesammelt werden, wodurch sie für nachfolgende Untersuchungen weiter zur …
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Advances in biophysical characterisation through micron scale flow engineering
… measurements on protein self-assembly in a label-free environment. I proceeded by addressing two challenges at the core of creating micron scale separation platforms - the integration of strong and stable electric fields with micron scale channels and the enhancing of the resolution limit of such …