Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 3 of 3 for “"FrbF"”.
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Toward the synthesis and evaluation of novel N-acylated FR-900098 analogs
… and characterized the N-acetyltransferase FrbF from the FR-900098 biosynthetic pathway, which was recently completely reconstituted in E. coli. This enzyme is responsible for transfer of the acetyl group from acetyl-CoA to the phosphonate substrate, representing the penultimate step in …
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Directed Evolution of Phosphite Dehydrogenase and Engineered Biosynthesis of Fr-900098
… (FrbH), an N-acetyltransferase (FrbF), a novel amide hydroxylase (FrbG), and a promiscuous nucleotide hydrolase (FrbI). The presence of cytidine 5'-monophosphate (CMP) conjugated phosphonate intermediates in the pathway also gives fascinating new insights into how phosphonate …
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Natural product discovery and engineering at the protein, pathway, and genome scales
… characterization of the N-acetyltransferase FrbF and the corresponding target for FR-900098 inhibition, Dxr from Plasmodium falciparum, we show that the novel FR-900098 derivatives can serve as more potent inhibitors. A platform for their biosynthesis is also established in Escherichia coli. …