Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"Frameshifting"”.
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Messenger RNA Destabilization by -1 Programmed Ribosomal Frameshifting
… studies have identified programmed -1 ribosomal frameshifting (-1 PRF) signals in eukaryotic genomic sequences, and suggested a role in mRNA stability. This work improves and extends the computational methods used to search for potential -1 PRF signals. It continues to examine four yeast -1 PRF …
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Early-stage drug discovery of HIV-1 frameshifting modulators
… drugs. During HIV-1 replication, the viral frameshifting element orchestrates expression of a specific ratio of the essential Gag and Gag-Pol polyproteins from overlapping reading frames. This process is vital for viral assembly and maturation, since altering the ratio of Gag and Gag-Pol …
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Recoding: reprogrammed genetic decoding with an emphasis on antizyme regulatory frameshifting
… Recoding events such as programed ribosomal frameshifting are especially plentiful in viruses. In most organisms only a few cellular genes are known to employ programed ribosomal frameshifting in their expression. By far the most prominent and therefore well-studied case of cellular +1 …
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The role of -2/-1 ribosome frameshifting mechanism in PRRS virus replication
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01
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Crystal structure and functional studies of a viral RNA pseudoknot involved in ribosomal frameshifting
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1999.
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Super-Resolution Force Spectroscopy for Revealing Sub-nucleotide Motion in Ribosomal Frameshifting and DNA Methylation
Programmed ribosomal frameshifting plays an important role for many viruses in their protein synthesis, with well-known cases including HIV and SARS-CoV-2. Aiming for an approach to defend against such viruses, techniques including X-ray crystallography, cryo-electron microscopy (Cryo-EM) and …
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STRUCTURAL STUDY OF HUMAN CATENIN ΒETA-LIKE PROTEIN 1 AND DOUBLE RNA PSEUDOKNOTS IN -1 RIBOSOMAL FRAMESHIFTING
… in the regulation of -1 programmed ribosomal frameshifting in RNA viruses −1 programmed ribosomal frameshifting (PRF) is utilized by many viruses to synthesize their enzymatic and structural proteins at a defined ratio. For efficient −1 PRF, two cis-acting elements are required--a …
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Multi-color visualization and quantification of single RNA translation and HIV-1 programmed ribosomal frameshifting in living cells
… modality was used to investigate ribosomal frameshifting during translation. Frameshifts occur through a ribosomal +1(-2) or -1 (+2) nucleotide(s) "slip" into another frame and are implicated in both human disease and viral infections. While previous work has uncovered many mechanistic …
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A Novel HIV-1 Genomic RNA Packaging Element and its Role in Interplay between RNAPackaging and Gag-Pol Ribosomal Frameshifting
… translation and genome packaging.The ribosomal frameshifting step of translation is critical for efficient synthesis of Gag and Gag-pol proteins through shifting of ribosome in -1 nt direction at the ribosomal frameshift signal (RFS). After identification of a Genomic RNA Packaging Element …
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Investigating and Reprogramming RNA Folding with Molecular Probes
… applied to pinpoint an interaction between the frameshifting stimulation element (FSE) and a sequence of RNA over one kilobase downstream, which occurs in nearly half of the RNA molecules. This technique is expanded to reveal long-range interactions in three additional coronaviruses, suggesting …
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RNA G-quadruplexes and polyamines effects on translation: data analysis and development of software for ribosome pause prediction
… expression at the translation level, ribosomal frameshifting, decoding rate, ribosome pausing etc. The process of translation can be affected locally by the sequence of translated mRNA, e.g. by specific secondary structures as well as globally by cellular conditions, e.g. concentrations of …
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Development of a ribosome profiling protocol to study translation in the yeast Kluyveromyces marxianus
… of mRNA translation including recoding (e.g. +1 frameshifting), ribosome stalling and differential gene expression between multiple conditions. In addition, as RPFs can be mapped back to the genome, novel translated ORFs may be discovered including novel protein coding genes and regulatory …
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A METHOD FOR IDENTIFYING RIBOSOME PAUSE SITES IN MESSENGER RNA THROUGH NEW SEQUENCING TECHNOLOGY
Ribosome frameshifting, pausing, and termination events have a profound effect on the accuracy and reliability of protein synthesis. A variety of translational control mechanisms combine to ensure the fidelity of this process. Mechanisms, such as aminoacyl-tRNA selection and codon:anticodon …
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Methods of single-molecule energy landscape reconstruction with optical traps
… to stimulate -1 programmed ribosomal frameshifting and we measure its structural properties. We measure the binding energy of a GA/AG tandem mismatch, one of many mismatches with unusual properties. We use our single-molecule methods to reproduce bulk measurements of the …
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Programmed genetic decoding with an emphasis on translational bypassing
… subdivided into several respective categories: frameshifting, bypassing, and redefinition. Recoding events are distinct from standard errors in translation due to the programmed fashion in which they occur, with their efficiency tuned by a set of cis-acting elements located 3’ and/or 5’ of the …
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Multilayered regulation of translation: roles of rRNA modifications, alternative initiation factors, and RNA-binding proteins
… alters ribosome dynamics, increases frameshifting, impairs internal ribosome entry site mediated translation, and compromises translational fidelity. Finally, the intrinsically disordered RNA-binding protein Sbp1 is examined for its role in translational regulation and mRNA storage. …
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Recoding and reassignment in protists
… I determined over 1,700 cases of ribosomal frameshifting (22% of genes analysed) in E. crassus. I identified 47 codons upstream of a stop signal which directs the ribosome to either the +1 or +2 reading frames. Termination only occurs in the context of the poly-A tail. In addition I analysed …
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BIOINFORMATICS INVESTIGATION OF RNA PSEUDOKNOTS
… their involvement in the −1 programed ribosomal frameshifting (−1 PRF) and stop codon readthrough translational recoding events as the stimulatory structures. In this dissertation, three large scale bioinformatics investigations were carried out on the roles of pseudoknots in the −1 PRF, as well …
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Discovery of the novel mouFSnrp gene and the characterisation of its in situ expression profile during mouse neurogenesis
… mouFSnrp, and its putative -1 translational frameshifting structure. Further, preliminary data presented in this thesis regarding the mouFSnrp in situ expression pattern during mouse brain development may suggest a key role of the gene in neuronal migration and neurogenesis in mice.
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The roles of papain-like protease-related proteins in viral replication and host immunity
… proteins including nsp2 and two ribosomal frameshifting products-nsp2TF and nsp2N share the same N-terminal PLP2 domain. In chapter 2, nsp2TF and nsp2N were demonstrated to be critical for host innate immunity suppression at least through the PLP2 domain-mediated deubiquitination and …
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