Global ETD Search
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Showing 1 to 18 of 18 for “"Four Helix Bundle"”.
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Structural and Functional Studies of Munc18 and SNARE Proteins
… families. SNAREs function by forming a four-helix bundle called SNARE complex; assembly of the SNARE complex brings two membranes together and is key for membrane fusion. The function of SM proteins is less clear. The neuronal SM proteins Munc18-1 was identified and linked to synaptic …
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Insights into the Functions of Munc18-1 in Neurotransmitter Release
… interacts with synaptobrevin and the SNARE four-helix bundle with week affinity, which have been shown to stimulate in vitro SNARE-dependent liposome fusion. Biophysical characterization of these two interactions could provide important information to uncover the roles of Munc18-1 in …
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Structural Studies of Complexin/SNARE Interactions
… the N-terminal half of SNARE complex forms a four-helix bundle, while the C-terminal half, starting just after the polar layer, is disordered. In the presence of the Syb2-CT, however, both halves of the SNARE complex form a four-helix bundle. It is interesting to note that Syb2 residues 29-84 …
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Synaptic structure and function at the Drosophila larval neuromuscular junction : a molecular analysis of complexin and radish
… is driven by the formation of a parallel four-helix bundle of SNARE proteins that are present on both the vesicular (v-SNAREs) and target plasma membranes (t-SNAREs). The full zippering of this bundle is thought to provide the driving force for membrane fusion. At synapses, vesicle fusion …
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De Novo Design And Engineering Of Functional Metal And Porphyrin-Binding Protein Domains
… DF2 into CDM13, a single chain di-Manganese four-helix bundle. CDM13 acts as a functional model of natural manganese catalase, exhibiting a k<sub>cat</sub> of 0.08s<sup>-1</sup> under steady-state conditions. The bound manganese cofactors have a reduction potential of +805 mV vs NHE, which is …
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Packaging and Secretion of Airway Mucins
… <p>Mucin exocytosis is mediated by a four helix bundle called the SNARE complex. Prior to complex formation, three of the four helices are located on the target membrane (t-SNARE) and one helix is vesicle bound (v-SNARE). Formation of the complex drives fusion of the exocytic vesicle …
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DNA Recognition by Helix-Loop-Helix Proteins
<p>The Helix-Loop-Helix (HLH) family of eukaryotic transcription factors comprises a large number of proteins which play key roles in homeostasis, the regulation cell proliferation, and differentiation. These proteins share a phylogenetically conserved bipartite b/HLH domain responsible for …
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The Advancement of Mass Spectrometry-based Hydroxyl Radical Protein Footprinting: Application of Novel Analysis Methods to Model Proteins and Apolipoprotein E
… form of which has been characterized as a four-helix bundle. Finally, we find by FPOP that ApoE interacts with beta-amyloid peptide 1-42 at a specific site in its N-terminal domain.
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Molecular Mechanisms Controlling Synaptic Vesicle Fusion
… this energy by zippering up into a parallel four helix bundle in a thermodynamically favored process. Because the zippering of SNAREs is spontaneous, fusion events occur immediately upon a vesicle interacting with its target membrane. But, in certain circumstances, such as in synaptic …
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The Structural and Functional Study of GIT1 Paxillin Binding Domain
… (NMR) spectroscopy. The PBD folds into a four-helix bundle, which is structurally similar to the focal adhesion targeting (FAT) domain and the vinculin tail (Vt) domain. The PBD is more stable than the FAT domain and there is no evidence of helix 1 swapping. <br /><br />Previous studies …
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Uncovering Biophysical Determinants Of Oxidoreductase Function Through De Novo Protein Design
… flavin activation in two classes of four-helix bundle proteins with complementary biophysical properties. This thesis discusses the implementation of both active sites in each of the two classes of scaffolds, and draws conclusions about the essential components of each. With the …
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Design und chemische Synthese von Häm- und Chlorophyll-bindenden Vier-Helix-Bündel-Proteinen
… Assembled Synthetic Protein), bei dem Vier-Helix-Bündel durch Kopplung von helikalen Peptiden auf ein solides Templat-Molekül dargestellt werden. Zu deren Funktionalisierung können Metall-Kofaktoren, z.B. Häm, in den hydrophoben Kernbereich eingelagert werden. Diese Strategie wurde zu einer …
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Storing copper in Streptomyces lividans: structural and biochemical properties of a copper storage protein
… found in the bacterial cytosol and comprise of a four-helix bundle that assemble into homotetramers and can bind between 70-80 Cu(I) ions through mainly Cys thiolate coordination. In Chapter 2 bioinformatic analyses reveals the phylogenetic distribution of Csp3 across Bacteria and Archaea and …
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Molecular modeling and simulation of bacterial chemosensory arrays
… including the previously uncharacterized TM four-helix bundle and HAMP domains. In addition, I report a series of MD simulations of a fragment of the resulting Tsr model, investigating the structural and dynamical effects of mutations on a key control cable residue. Preliminary MD simulations …
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Toxin-membrane interactions of Pasteurella multocida toxin and homologous domains
Toxin-membrane interactions are poorly understood on the molecular level due to the inherent difficulty of crystallizing membrane protein complexes and the large size (>50 kDa) of most functional AB or MARTX protein toxins. Toxins can interact with membranes at three stages during intoxication: 1) …
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Design, Synthesis and Characterization of Heme-proteins: Developing Potential Catalysts for Bio-remediation
The next generation of toxic chemicals and hazardous wastes from sophisticated chemical industries will demand the environmental agencies to employ biological methods over the conventional physical and chemical remediation methods. Over the past decade, natural metallo-enzymes have been identified …
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Einfluss der endo-lysosomalen Qb-SNAREs Vti1a und Vti1b auf die subzelluläre Sortierung sowie auf signalgebende Prozesse im zentralen Nervensystem der Maus
… which is mediated by SNARE proteins forming a four-helix bundle to provide the driving force necessary for fusion. The Qb-SNAREs Vti1a and Vti1b are known to be implicated in several endo-lysosomal fusion processes. In mice, the single knockout of either Vti1a or Vti1b is associated with only …
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Coordination Geometries in Metallobundles Enforcing Oxidative and Hydrolytic Catalysis and Designing Biomaterials for Use As Antimicrobials
… in simpler, yet robust model constructs. Helical bundles serve as premier scaffolds to incorporate diverse reactivities from oxidative, reductive, to hydrolytic transformations observed in much grander O2-utilizing metalloproteins such as radical-generating ribonucleotide reductases and catalases. …