Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 73 for “"Forskolin"”.
-
Ire1 regulates cyp11a1 transcription to mediate steroidogenesis in the ovary
… stress response is not induced in KGN cells by forskolin treatment. We hypothesize that IRE1 is necessary to mediate CYP11A1 transcription in response to forskolin, a gonadotropin mimetic, in a mechanism independent to its role in ER stress. In the human KGN granulosa cell line, IRE1 is not …
-
CFTR klórgöng í ristilþekju hænsnfugla (Gallus gallus)
… Efnunum glúkósa, NPPB (klórgangahindi) og forskolin (CFTR örvari) bætt út í baðið í mismunandi röð til að kanna virkni klórganganna. Niðurstöður: Glúkósi hafði engin marktæk áhrif á jónaflutning yfir ristilþekjuna. NPPB hafði marktæk neikvæð áhrif á jónaflutning yfir ristilþekjuna og …
-
The role of Ca²⁺ and cAMP in GnRH-stimulated LH release
… entry via VSCC (about 50%) and ROCC (about 50%). Forskolin, an activator of adenylate cyclase, was used to investigate the role of cAMP in LH release. Forskolin stimulated an increase in both LH release and cellular cAMP levels. GnRH was also able to elevate the cellular CAMP concentration. GnRH …
-
Cyclic AMP and the Induction of Reactive Astrogliosis
… modeled in vitro by treatment of F98 cells with forskolin, an activator of adenylyl cyclase. Here I tested the hypothesis that a cAMP-mediated intracellular pathway is involved in eliciting forskolin-induced reactivity in F98 cells, and I attempted to elucidate that pathway using inhibitors of …
-
Diversity of transduction mechanisms in receptor neurons of the main olfactory epithelium in <i>Xenopus laevis</i> tadpoles
… cAMP-vermittelten Transduktionsweg aktivieren (Forskolin und membranpermeables cAMP). Die Reizantworten wurden mittels der "patch-clamp" Technik und "calcium imaging" gemessen. Sowohl Aminosäuren als auch die Wirkstoffe, die den cAMP-vermittelten Transduktionsweg aktivieren, erwiesen sich als …
-
Autocrine regulation of gonadotropin-releasing hormone in immortalized hypothalamic GT1-7 neurons
… and was enhanced by the addition of K⁺, L-GLU, forskolin and PMA. Furthermore, K⁺- and forskolin-induced GnRH release was dependent on extracellular Ca²⁺. For the analysis of an ultrashort feedback mechanism, GT1-7 cells were cultured in 6-well plates to near confluence and then incubated in …
-
Adrenergic-cholinergic interactions in the heart
… to isoproterenol, a f3-adrenoceptor agonist, forskolin, a direct activator of adenylate cyclase, IBMX, a phosphodiesterase inhibitor, and phenylephrine, an a-adrenoceptor agonist. While the inhibitory effect of carbacholon the isoproterenol-stimulated cAMP generation is believed to contribute …
-
Epac-mediated modulation of neurotransmitter release from cultured hippocampal neurons
… Epac Aktivierung konnte eine Forskolin vermittelte Erhöhung von Aktionspotential evozierten exzitatorischen postsynaptischen Stromamplituden im Durchschnitt zu 38 % und die Forskolin-vermittelten Zunahme der Anzahl spontaner Vesikelfusionen zu 100 % erklären. Allgemein wird …
-
High throughput optical sensor arrays for drug screening
… the cells were exposed to Isoproterenol and Forskolin, which are known to elicit intracellular cyclic AMP production. The assay proved robust in detecting long term drug induced extracellular potassium flux. Ion flux magnitude was used to generate EC50 values of 1.185 nM and 66.5 nM for …
-
Purification of protein phosphatases and 14-3-3-binding proteins and their regulation by the cAMP–PKA pathway
… from cellular extracts. I used the cAMP agonist forskolin to trigger activation of the cAMP–PKA pathway and used the H-89 inhibitor to block PKA activity. Although several protein phosphatases were identified along with their regulatory subunits, I could not find any striking enrichment of …
-
Adenylyl Cyclase Type 9: Regulation and Cardiac Function
… including G-proteins, protein kinases, and forskolin utilizing in vitro and cell based assays. Overall, I conclude that most G-proteins or protein kinases do not directly regulate AC9, except Gαs, in vitro. Although AC9 is forskolin insensitive alone, weak activation by forskolin in the …
-
The human cannabinoid CB(1) receptor stably expressed in Chinese hamster ovary cells provides a model system to predict the pharmacological effects of cannabinoids in man
… to be full agonists by maximally inhibiting forskolin-stimulated cAMP formation in CHO/CB₁ cells. The finding that SR141716A is an inverse agonist was further supported by its ability to augment forskolin-stimulated cAMP formation. Interestingly, Δ⁹-THC was a partial agonist in this assay …
-
Lipid Quantification and Cryopreservation of In Vitro Produced Jersey Cattle Embryos
… is associated with impaired embryo quality. Forskolin is an adenylate cyclase activator that regulates cAMP levels in cells and has been shown to induce lipolysis in IVP embryos. L-carnitine is required for transport of fatty acids from the intermembrane space of the mitochondria into the …
-
Myocardial Contractility in Fetal Sheep Exposed to Long-term Hypoxia at High Altitude : Activator Calcium and Beta-adrenergic Receptor Function
… (10<sup>-10</sup>-10<sup>-6 </sup>M), and forskolin (0.01-10 uM) in isometrically contracting papillary muscles. In addition, we quantified dihydropyridine (DHPR), ryanodine (RyR), and β-adrenergic receptor densities, and measured basal and stimulated intracellular cAMP levels. In hypoxic …
-
Dielectric Relaxation Processes at Apical Membranes of Frog Skin: Changes of Capacitance and Sodium Ion Channel Density
… stimulation of Na$\sp+$-channel density with forskolin (Els and Helman 1991) which caused a reversible increase of C$\sb{\rm a}$(dc) by $\approx$ 0.18 $\mu$F/cm$\sp2$. Inhibition of Na$\sp+$-channel density with quinine (Kizer, 1990) caused a decrease of C$\sb{\rm a}$(dc) by $\approx$ 0.32 …
-
5-HT1A receptor expression : Studies in postmortem tissue and characterisation of a model system.
… the 5-HT[1A] agonist 8-OH-DPAT, inhibited forskolin stimulated Ca[2+] release in the SHSY-5Y cell line and whether the 5-HT[1A] antagonist p-MPPI reversed this effect using flow cytometry. The post-mortem study showed that the G-1019 allele had significantly higher 5-HT[1A] expression …
-
The regulation of expression level in NeuroD/BETA2 by PKA
… of NeuroD/BETA2. Treatment of 293T cells with forskolin overexpressed NeuroD/BETA2, expression of NeuroD/BETA2 was increased. And NeuroD/BETA2 could be phosphorylate at N-terminous region by PKA. We predicted that phosphorylation site of NeuroD/BETA2 was at Thr91, using databases: Netphos, …
-
Allosteric Determinants of Guanine Nucleotide Binding Proteins and Methods to Crystallize the Cytosolic Domains of Adenylyl Cyclase
… of adenylyl cyclase bound to activators Gsa and forskolin were previously determined. However, structural information regarding low activity (non-Gsa or forskolin bound) states of the enzyme is lacking. Genetic and biochemical methods were utilized to overcome the low affinity of the cytosolic …
-
Fluid retention in congestive heart failure-the role of natriuretic peptides and arginine vasopressin
… were done in IMCD cells upon addition of AVP and forskolin. ANF, BNP and CNP radioligand studies were performed in glomerular membrane. Results showed that baseline cGMP/creatinine ratios and the plasma ANF levels were higher in the cardiomyopathic hamsters. The plasma ANF levels correlated with …
-
The involvement of second messenger signalling in impaired integrin function of HIV-1 infected macrophages
… wurden Phagen 7 Tage nach Infektion mit Forskolin (10mins, 0.01µM to 100µM), 2’-5’-Dideoxyadenosin (ddAD, 30min, 0.1µM to 10µM), oder MDL-12,330 A (30mins, 0.1µM to 5µM) vorbehandelt. Um das Verhalten der Zellen in Abwesenheit aktiver retroviraler Replikation zu messen, benutzten wir …
Page 1 of 4