Global ETD Search
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Showing 1 to 11 of 11 for “"FokI"”.
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Analysis of FokI Cleavage Resistance Observed in DNA Sequences Generated via the Combinatorial Selection Method, REPSA
<p>FokI is a thoroughly investigated and highly utilized restriction endonuclease that recognizes the DNA sequence, 5’-GGATG-3’, and cleaves outside of this site 9 and 13 base-pairs downstream. The shifted cleavage function possessed by this kind of endonuclease is utilized in many applications …
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Yüksek kan kurşun düzeyi olan işçilerde vitamin D reseptör gen polimorfizminin kan ve idrar kurşun düzeylerine etkisi
… yüksek kan kurşun düzeyine sahip kişilerde VDR FokI başlangıç bölgesindeki C→T tek nükleotid polimorfizminin kan ve idrar kurşun düzeyine etkisi araştırıldı. İzole edilen DNA'ların VDR FokI başlangıç bölgesi PCR tekniği ile çoğaltılarak, PCR ürünü FokI restriksiyon enzimi ile kesildi ve …
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Influence of Vitamin D Receptor Gene Polymorphisms on Changes in Insulin Sensitivity with Aerobic Exercise Training
… of the study was to examine the influence of the FokI and BsmI polymorphisms of the vitamin D receptor gene on changes in insulin sensitivity with aerobic exercise training in men and women at increased risk for type 2 diabetes mellitus (T2DM). Subjects were genotyped and underwent oral glucose …
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Estudo da influência da variabilidade em genes de apolipoproteínas sobre níveis lipídicos e parâmetros de massa e gordura corporal na população de Porto Alegre
… C-I (HpaI), C-II (AvaII), C-III (SacI, FokI e MspI) e A-IV (XbaI, HinfI e PvuII). A amostra foi composta por 391 indivíduos caucasóides de Porto Alegre (RS) e dados sobre hábitos de vida, dosagens lipídicas e medidas antropométricas foram obtidas para cada indivíduo. Os fragmentos de …
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Engineering of transcription activator-like effector nucleases (TALENs) for targeted genome editing
… by fusing the DNA binding domain of TALE and a FokI non-specific DNA cleavage domain. TALEs are isolated from the plant pathogenic bacteria from the genus Xanthomonas and their DNA binding domains are composed of a series of tandem repeats. Each repeat comprises 33–35 amino acids and recognizes …
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Entwicklung, Modellierung und Aufbau des 10 Joule-Vorverstärkers mit adaptiver Optik für den Petawatt-Laser PHELIX
… durch Vorkompensation der Wellenfrontstörungen Foki nahe dem Beugungslimit erreicht.
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The Design and Development of Artificial Zinc Finger Transcription Factors and Zinc Finger Nucleases to the hTERT Locus
… DNA binding domain (ZFP) linked to the FokI nuclease domain by a short amino acid “inter-domain linker”. The general sequence motif of a ZFN target is 5’-(ZFN site1)-(6 bp spacer)-(ZFN site2)-3’ and each half-site is 5’-GNNGNNGNN-3’. Variations of this motif come in the forms of variable …
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Optimizing Zinc Finger Nucleases for Use in Mammalian Cells
… binding domain to the nuclease domain from the FokI restriction endonuclease and have been used to induce double strand breaks at precise sequences. Although ZFNs have been successfully used to stimulate gene targeting at specific loci, several issues remain. First, a generalized optimal design …
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Mechanistic Understanding of the Role of TRF1 in Telomere Replication
… fragile telomere formation in cells with FokI-induced telomeric DSBs and in alternative lengthening of telomeres (ALT) cells, which have spontaneous telomeric damage. BIR of telomeric DSBs competes with PARP1-, LIG3-, and XPF-dependent alternative non-homologous end joining (alt-NHEJ). …
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Functional characterization of poly(ADP-ribose) dependent ubiquitin E3 ligase in the DNA damage response
… 25 19. Electro-mobility shift assay 26 20. FokI assay 26 21. Biotin pull down assay 27 22. Purification of recombinant protein from insect Sf9 and bacteria cells 27 23. Statistical analysis 28 Ⅲ. RESULTS 29 Part A. Systematic screening of pADPr dependent E3 ligase functions in the DNA damage …
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Genetic strategies to manipulate meiotic recombination in Arabidopsis thaliana
… normally mature into crossovers. Instead, TALFokI nucleases (TALENs) were expressed from meiotic promoters in order to generate exogenous DSBs at sites determined by the DNA binding specificity of the TAL repeat domains. The project succeeded in transforming TALENs into spo11-1 mutants and …