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Showing 1 to 20 of 48 for “"Flux Analysis"”.
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Metabolic flux analysis in mammalian cell culture
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1993.
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Metabolic flux analysis and population heterogeneity in mammalian cell culture
Metabolic flux and population heterogeneity analysis were used to develop relations between mammalian cell physiology and specific culture environments and to formulate strategies for increasing cell culture performance. Mitochondrial characteristics associated with respiration, membrane potential, …
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¹³C-metabolic flux analysis of recombinant yeasts for biofuels applications
… efforts can be facilitated by ¹³C-Metabolic Flux Analysis (MFA), a method for elucidating the otherwise unobservable intracellular metabolic fluxes in biological systems. We first developed protocols for extraction and LC-MS/MS analysis of intracellular metabolites, which provides data that …
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Exploring cancer metabolism through isotopic tracing and metabolic flux analysis
Cancer is the second leading cause of death following heart diseases in the U.S. During the past two decades, cancer metabolism has emerged as an indispensable part of contemporary cancer research. Various types of metabolic alterations in cancer cells have been documented, prompting extensive …
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Nonstationary metabolic flux analysis (NMFA) for the elucidation of cellular physiology
… understanding therein. A cell's metabolic flux distribution has been proposed to be one of the most solid and meaningful indicators and descriptors of metabolism. Metabolic fluxes represent integrative information and are a function of gene expression, translation, post-translational …
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Branchpoint flux analysis in the L-lysine pathway of Corynebacterium glutamicum
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1997.
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Metabolic flux analysis of Escherichia coli MG1655 under octanoic acid (C8) stress
… of large amount of SCFAs, 13C metabolic flux analysis of E. coli was performed for both C8 stress and control condition (without C8). To investigate central carbon metabolism for the culture environmental change, a mixture of labeled and unlabeled glucose was used as the sole carbon …
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Carbon-13 metabolic flux analysis of soybean central carbon metabolism: response to temperature and genotype effects
… changes in the composition are unknown;Metabolic flux analysis (MFA) quantifies carbon flow in a biological system, which is an important characteristic reflective of the system physiology. The application of MFA to study complex plant metabolic networks has however been recent. Towards this goal, …
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Identification of branch-point restrictions in microbial metabolism through metabolic flux analysis and local network perturbations
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1991.
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Gluconeogenesis as a system : development of in vivo flux analysis of hepatic glucose production in Type 2 Diabetes
… to reconstruct hepatic glucose production fluxes by infusion of [13C, 2H]-glycerol and mass spectrometry analysis of plasma metabolites. Using this methodology we observe physiologic changes in liver cell lines and primary hepatocyte cultures in the presence of hormones insulin/glucagon and …
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Development and Application of Fluxomics Tools for Analyzing Metabolisms in Non-Model Microorganisms
… transcriptomics, proteinomics and metabolomics analysis of microbial metabolism, fluxomics tools can measure and simulate the in vivo enzymatic reactions as direct readouts of microbial metabolism. This dissertation develops and applies broad-scope tools in metabolic flux analysis to investigate …
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Development of Steady-State and Dynamic Flux Models for Broad-Scope Microbial Metabolism Analysis
<p>Flux analysis techniques, including flux balance analysis (FBA) and 13C-metabolic flux analysis (MFA), can characterize carbon and energy flows through a cells metabolic network. By employing both 13C-labeling experiments and nonlinear programming, 13C-MFA provides a rigorous way of examining …
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Experimental characterisation of bubbly flow using MRI
… of a model bubble column using drift-flux analysis by supplying experimental closure for those parameters which are considered difficult to measure by conventional means. The system studied consisted of a 31 mm diameter semi-batch bubble column, with 16.68 mM dysprosium chloride …
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Computational Modeling of Planktonic and Biofilm Metabolism
… approaches (e.g., transcriptomics and proteomics analysis). However, these approaches are limited to provide the direct description of intracellular metabolism (e.g., metabolic fluxes) of microorganisms. Therefore, in this study, I have applied computational modeling approaches (i.e., 13C assisted …
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Fermentative utilization of glycerol and lignocellulosic sugars and production of ethanol by Paenibacillus macerans
… of ethanol by P. macerans. Further, metabolic flux analysis (MFA) was used as a systemwide tool to understand the role of various metabolic pathways in the fermentative utilization of these substrates. Glycerol is a byproduct of biodiesel and bioethanol production, which is an abundant, …
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Biochemical Thermodynamic Modelling of Cellular Bioenergetics: A Quantitative Systems Pharmacology Approach
… with experimental in vitro extracellular flux analysis in order to assess drug redox cycling and cellular bioenergetics. It is widely accepted that pharmacological activity of certain classes of drugs (e.g. anticancer, antimalarial) is related to their ability to accept one or two …
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Comprehensive analysis of metabolic pathways through the combined use of multiple isotopic tracers
Metabolic Flux Analysis (MFA) has emerged as a tool of great significance for metabolic engineering and the analysis of human metabolic diseases. An important limitation of MFA, as carried out via stable isotope labeling and GC/MS measurements, is the large number of isotopomer equations that need …
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Investigating Non-model Microorganisms for Biomanufacturing via Systems Biology and Machine Learning
… genome-scale modeling techniques for 13C flux analysis.
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Methodologies and analysis of metabolic flux in mammalian systems
<p>Quantification of metabolic fluxes has broad applications in studying metabolic physiology. Isotope tracing with heavy labeled carbon (13C labeled metabolic flux analysis, 13C-MFA) is a promising strategy due to its ability to compute metabolic fluxes from isotope tracing profiles without …
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