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Showing 1 to 20 of 28 for “"Fluorometer"”.
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Ein neues Fluorometer zum Nachweis organischer Stoffe im Meer
… Gründen ist das Ziel dieser Diplomarbeit, ein Fluorometer für gelöste organische Materie (dissolved organic matter = DOM) zu entwickeln, das die neueste Lichtquellengeneration ausnutzt. Es wurde ein neues Fluorometer mit dem Name FluDOM entworfen. Es ist zur Zeit nur für die Detektion von …
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Variability of phytoplankton production rates in the Atlantic Ocean as observed using the Fast Repetition Rate Fluorometer
… a novel instrument, the Fast Repetition Rate Fluorometer (FRRF). The underlying theory and use of this instrument is described in detail. High resolution FRRF data collection was performed during three oceanographic cruises: RV <i>Pelagia</i>, March 1998, RRS <i>James Clark Ross</i>, May-June …
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Nutrient limitation dynamics of a coastal Cape Cod pond : seasonal trends in alkaline phosphatase activity
… throughput by 75% compared to a standard filter fluorometer method. The detection limit, capability to measure APA at different time intervals, and performance at sea were tested. APA measurements made using the cytofluor were comparable to those made using a standard filter fluorometer, thus …
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Using low cost components to determine chlorophyll concentration by measuring fluorescence intensity
… describes the development of a low cost fluorometer with the aim of using it as an algae and phytoplankton concentration sensor. As it forms the core of this fluorometer's functionality, chlorophyll's fluorescence characteristics and origins are discussed. Special attention is given to …
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Nutrient limitation dynamics of a coastal Cape Cod pond : seasonal trends in alkaline phosphatase activity
… throughput by 75% compared to a standard filter fluorometer method. The detection limit, capability to measure APA at different time intervals, and performance at sea were tested. APA measurements made using the cytofluor were comparable to those made using a standard filter fluorometer, thus …
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Exploring the Structural and Functional Characteristics of a Catalytic DNA
… sources when used in conjunction with a handheld fluorometer. Detection limits under these conditions were lower than 72 nM, which is the EPA standard for Pb2+ in drinking water. An electrochemical Pb2+ sensor has also been developed by conjugating a ferrocene moiety to a gold electrode …
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Design and construction of a Helmholtz coil apparatus for nanoparticle heating
… can manipulate a sample of biomolecules within a fluorometer.
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Synthesis and solvatochromic investigation of 1,4-Di isocyanonaphthalene
… For the fluorescent spectroscopy method the fluorometer was usedto record the emission and excitation spectrums.
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Rapid measurements of periphytic responses to nutrients using PAM fluorimetry
… the use of a pulse amplitude modulated (PAM) fluorometer for measuring biomass and photosynthetic activity in conjunction with NDS over several weeks. The results of this study suggest that this is a comparable methodology not only for measuring periphytic responses to nutrients but also for …
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Studies of Surfactants Effect on Riboflavin Fluorescence and Its Determination in Commercial Food Products and Vitamin Tablets.
<p>A simple and economical fluorometer using blue LEDs excitation sources and simple PMT detection had been built, assembled, optimized, and employed for measurement of fluorescence from riboflavin (vitamin B<sub>2</sub>). Surfactants have been known to enhance the intensity of fluorescence of …
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Large-scale dynamic observation planning for unmanned surface vessels
… ahead of the path of a hurricane, and collecting fluorometer readings to observe and track a harmful algal bloom.
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Fluorometric determination of sulfhydrl plus disulfide as an index to heat treatment of half-and-half
… acetate. Percent quenching was determined with a fluorometer at exciting wavelengths of 415 to 436 nm and 515 nm for measurement. Calculations were made according to the formula: µmoles of -SH + -SS- (X) = percent quenching (Y)/10,256 + 0.00068. Heat treatments of half-and-half between 65.6°C and …
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Are Mussel Shells Environmental DNA Time Capsules? A Comparison Of Extraction Methods For Obtaining DNA From Shell Material.
… the extractions was quantified using a Qubit 3.0 Fluorometer and a Nanodrop 2000. A modified CTAB extraction method was found to be statistically higher for extracting total DNA compared to the other three methods. This suggests that chloroform-based extractions may be optimal for DNA extraction …
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Characterization of Free Oxylipins in Extracellular Vesicles from Bovine Milk
… Protein content was assessed using a Qubit 4 fluorometer, and oxylipin analysis by LC/MS. Descriptive statistics and nonparametric testing compared oxylipin levels across treatments. Results. Twelve oxylipins were detected in bovine milk and EVs. 9-HODE (!2 =0.92) and 15- HEPE (!2=0.91) were …
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Design and construction of an apparatus for induction heating for controlling DNA hybridization
… analyzer and through scans conducted on a fluorometer. The initial design requirements for the coil were that it needed to heat the nanoparticle, should be suitable for optical testing, and require a relatively small sample volume. At the end of the design and testing period, however, a …
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Towards molecular autopsies: Internal validation of the Qubit 1 X dsDNA HS Assay Kit
… the Qubit™ 1X dsDNA HS Assay Kit on the Qubit™ 4 Fluorometer as part of a molecular autopsy workflow for forensic applications. The Qubit™ 1X dsDNA HS Assay workflow was optimised and then used to assess the DNA concentration of control Lambda DNA, extracted DNA from forensic samples and DNA …
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Spectral Fluorometric Characterization of Phytoplankton Community Composition and Biomass, Chromophoric Dissolved Organic Matter, and Photosynthetic Capacity Using the Algae Online Analyzer
… fixed wavelength spectral fluorometer, the Algae Online Analyzer (AOA), which is designed and marketed for continuous monitoring purposes. Monospecific algal cultures and natural waters from Oyster Landing, North Inlet, were used to test the AOA's ability to measure total …
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Changes in Skeletal Muscle Sarcoplasmic Reticulum Function in Adult and Aged Fisher 344 Brown x Norway Rats
… and six in group 27 months. A Jasco CAF-110 Fluorometer and fura-2 were used to determine the rate of Ca2+ uptake and release by isolated SR vesicles. In the aged animals, mass of the soleus was reduced by 22%, while the plantaris was reduced by 23%, and the diaphragm by 15%. However, these …
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Effects of Temperature stress on coral fluorescence and reflectance
… in both A. cervicornis and O. annularis. Fluorometer analyses further demonstrated that the photosystems of the algae (PSII) were damaged by exposure to higher water temperatures, implying that the immediate effect of the heat stress was on them, as opposed to the hosts. These results …
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Dissolved organic material in groundwater and application of fluorescence spectroscopy coupled with EEM PARAFAC models as a means of site characterisation
… systems. The second stage utilised a hand held fluorometer to identify and delineate a landfill leachate plume at concentrations as low as 1%. In the third stage, fluctuations of dissolved organic carbon (DOC) were measured in groundwater during a high intensity abstraction event with …
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