Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 51 for “"Fluorogenic"”.
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SENSORS BASED ON IMMOBILIZED FLUOROGENIC REAGENTS
… based on the fluorescence of immobilized fluorogenic reagents are prepared and their characteristics are defined. The fluorogenic reagent is chemically immobilized on a solid support and attached to the common end of a bifurcated fiber optic bundle. Fluorescence is excited through one arm …
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SYNTHESIS OF CHROMO FLUOROGENIC SENSORS FOR MOLECULAR RECOGNITION
This work aims to explore the possibility of design, synthesize and test capabilities, firstly in solution and then into the solid state later on in the form of monolayer new molecules, that present the ability to recognize a substrate and to transform this recognition event into a detectable …
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Fluorogenic probes for live-cell imaging of biomolecules
Fluorogenic probes, small-molecule sensors that unmask brilliant fluorescence upon exposure to specific stimuli, are essential tools for chemical biology. Probes that detect enzymatic activity can be used to illuminate the complex dynamics of biological processes at a level of spatiotemporal detail …
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Single-Enzyme Kinetics with Fluorogenic Substrates: Strategies Towards Accurate Single-Turnover Detection
Contains fulltext : 161454.pdf (Publisher’s version ) (Open Access)
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Approaches towards development of novel fluorogenic biosensors for detection of small protein analytes
… approaches toward the development of a set novel fluorogenic biosensors capable of detecting native ErbB-ligands are reported. Using yeast surface display, two libraries of protein-binding scaffolds were engineered to bind selectively to human EGF and human betacellulin with low nM KD. …
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Trimethoprim-Based Chemical Tags for High Resolution Live Cell Imaging
… and subsequently rendered covalent and fluorogenic via proximity-induced protein labeling reactions. To date, the TMP-tag is one of the few chemical tags that enable intracellular protein labeling and high-resolution live cell imaging. In this dissertation I first focused on the …
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Synthesis and evaluation of aziridine and symmetry-based epoxide affinity labels for HIV-1 protease
… HIV-1 protease, based on an internally-quenched fluorogenic substrate. Of eleven compounds assayed for reversible inhibition, five demonstrated modest to potent activities (K$\rm\sb{i}$'s ranging from 70 $\mu$M to 10 nM).
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Nuevas sondas cromo-flurogénicas a partir de derivados organopaladio y compuestos indénicos
… medioambientales. _____________________________ Fluorogenic and chromogenic molecular chemical sensors have become extremely important in the chemical science. These systems are doubly interesting; first from the point of basic research, they study the host-guest interactions, mediated by a …
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Design and Use of Fluorescent Assays to Study Plasminogen Activator and Other Serine Proteases Involved in Biological Control
… of these fluorescent dyes were designed as fluorogenic substrates for the amidase and esterase activity of serine proteases. As a consequence of their unique spectral and biochemical properties, these derivatives represent the most sensitive synthetic substrates for serine proteases yet …
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A Molecular Basis for the C-Domain Selectivity of Angiotensin-Converting Enzyme
… interactions using domainselectiveinhibitors and fluorogenic peptides. Mutants tested with the fluorogenic peptidesdisplayed minimal, if any, acquisition of N-domain-like catalytic properties. Of the singlemutations, S2 (F391Y, tACE numbering) and S1 (V518T) pocket substitutions caused thelargest …
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High-throughput screening for ADP-ribosyl hydrolase 3 inhibitors
… a high-throughput screen was conducted using a fluorogenic substrate to discover an inhibitor of ARH3. A total of 496 primary hits have been identified and will be further studied to validate their ability to inhibit ARH3.
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Design and synthesis of probes for detection of protein-protein interaction and RNA localization
… for use in the solution phase preparation of a fluorogenic ribozyme substrate, and later immobilization of this substrate on a silyl resin for direct ribozyme selection.
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Development and application of chemical tools for investigating dynamic processes in cell migration
… using two wavelengths of light. Finally, a fluorogenic sensor to monitor the real-time activity of the GTPase Cdc42, an essential regulator of migratory processes, has been developed. The solvatochromic fluorophore 4-N,N-dimethylamino-1,8-naphthalimide has been incorporated into a protein …
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Studies on oligopeptidase B of Leishmania major
… it was decided to use short peptidyl fluorogenic substrates, which only fluoresce once the bond linking the peptide to the fluorescent moiety is cleaved. These allow detection of peptidase activity by quantifying the release of the fluorescent moiety. Detection of peptidase activity in …
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Characterisation of the structural motifs Involved in the cleavage and secretion of human angiotensin-converting enzyme
… in an even greater increase in shedding. Soluble fluorogenic peptides mimicking the ACE stalk were used in a cell-based assay to characterise the contribution of the stalk to ACE shedding. Hydrolysis of the wild-type peptide Abz-NSARSEGPQ-EDDnp was not responsive to phorbol ester or the …
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Investigation of the YefM-YoeBSa toxin-antitoxin systems as novel antibacterial targets
… the activity of YoeBSa1 led to the design of a fluorogenic substrate that can be used to screen for activators of this toxin in a high-throughput manner.
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Angiotensin-converting enzyme cleavage of the Alzheimer's beta-amyloid peptide
… activity and affected domain-selectivity. Two fluorogenic substrates, designed specifically to assess ACE's mechanism of Aβ hydrolysis Aβ(4-10)Q and Aβ(4-10)Y, underwent endoproteolytic cleavage at the Asp7/Ser8 bond. The Aβ(4-10)Q peptide was a poor substrate of ACE but was N-selective, with a …
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The Role of The Plasminogen-Fibrin Interaction in The Regulation of Fibrinolysis
… labelled plasminogen. A synthetic, fluorogenic substrate for plasmin was employed in the kinetic studies. Both forms of plasminogen displayed a physiologically relevant affinity for fibrin and limited plasmic cleavage of fibrin created new binding sites for plasminogen. This …
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Microfluidic temporal cell stimulation
… stimulant concentration profiles with real-time fluorogenic monitoring of cell physiological response. While cellular assay automation has been demonstrated in microfluidic devices, applying microfluidics to more difficult, multi-parametric systems biology problems, like the analysis of signal …
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Real time PCR measurement by fluorescence anisotropy
… complexity and cost are reduced compared to fluorogenic probe methods since the probes are eliminated. The design of a prototype instrument, which successfully implements this new method, is presented. Instrument and assay performance are compared to intercalating dye assays run in …
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