Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"Fluorimetry."”.
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Multiplexed Photometry And Fluorimetry Using Multiple Frequency Channels
… </p> <p> <strong>MULTIPLEXED PHOTOMETRY AND FLUORIMETRY USING MULTIPLE FREQUENCY CHANNELS</strong> </p> <p>by</p> <p> <strong>KHALED M. DADESH</strong> </p> <p>August 2013</p> <p> <strong>Advisor</strong>: Dr. Amar Basu</p> <p> <strong>Major</strong>: Electrical Engineering</p> <p> …
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Rapid measurements of periphytic responses to nutrients using PAM fluorimetry
<p>Water quality monitoring has traditionally been done by measuring periphytic algal biomass that has grown on fertilized or unfertilized patches of habitat produced by nutrientdiffusing substrata (NDS). This method requires the destruction of the accumulated periphyton communities and thus does …
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Novel Biophotonic Imaging Techniques for Assessing Women's Reproductive Health
… instrument, which uses simultaneous imaging of fluorimetry and multiplexed low coherence interferometry (mLCI) to measure in vivo microbicide gel thickness distributions. The study explores the optical performance of the device and provides proof of concept measurements on a calibration socket, …
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The Use of High Pressure Fluorescence Spectroscopy to Investigate Subunit Interactions in Oligomeric Proteins (Neurophysin, Lac Repressor)
… polarization and photon counting scanning fluorimetry.
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Engineering nitrilases for enhanced thermostability
… validation using nanoscale differential fluorimetry, negative stain and cryogenic electron microscopy, and single-particle analysis. In this study, atomic resolution structures of wild-type CynDpum and its variant CynDpum (Q86R/H305K/H308K/H323K) were employed to predict, using empirical …
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CE-LIF ANALYSIS OF INTACT MARINE MICROBES ALONG WITH THEIR CONSTITUENT PHYCOBILIPROTEINS AND PHYCOBILIN PIGMENTS
… of these proteins were themselves studied by fluorimetry after liberation from the proteins by way of chemical cleavage with DTT or TCEP. Whereas conventional CE was unable to distinguish between two Synechoccocus sp. bacteria (CCMP 1333 and CCMP 833) in a mixture, the new PectI method was …
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Building a rational model for the identification of allosteric sites
… for DYRK2 over DYRK1A by Differential Scanning Fluorimetry (DSF). This oxyamidine hit was further investigated through a structure activity relationship (SAR) which enabled the synthesis of three compounds that showed a higher stabilization than the initial hit (Tm > 1.2 °C). A second library …
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Inhibition of enzymes from mycobacteria using fragment-based approaches
… utilised, including both differential scanning fluorimetry and isothermal titration calorimetry. This approach led to the development of novel inhibitors with low nanomolar affinity. Select compounds were screened by collaborators against both Mab and Mtb in vitro. In light of encouraging …
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Development and Characterization of a Stopped-Flow-Bypass Analysis System With Applications To Biochemical Measurements
… for ultraviolet/visible spectrophotometry, fluorimetry, and chemiluminescence. Enzyme activities and fundamental enzyme kinetic parameters (Kms, Kis, VMAXs) were determined directly. Genetic transcription levels of luciferase in whole intact E. coli cells were also determined using …
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The Development of Assays for Detecting Proximal Phosphorylation and Covalent Modification of Proteins
… with a variety of assay types including fluorimetry, microscopy, polyacrylamide gels and flow cytometry. The second objective in assay development was to assess a modular electrophilic warhead platform for novel covalent inhibitors. Through nucleophilic aromatic substitution methodology, …
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Anthraquinone-peptide conjugates as inhibitors of DNA transcription factor binding.
… of the peptide conjugates with DNA using fluorimetry and thermal denaturation studies indicated that the AP-I inhibitors were weak intercalators of DNA and suggested that DNA binding of these intercalator-linked peptides was govemed~ in principle, by the peptidic moiety. Electrophoretic …
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Reevaluating Chlorotoxin Targets: Insights Into Glioblastoma Cell Migration and Neuropilin-1 Binding
… were utilized such as nanoDifferential Scanning Fluorimetry (nanoDSF) and Surface Plasmon Resonance (SPR). These analyses revealed that Ctx and its C-terminal fragments exhibit weak binding to NRP-1 at high micromolar affinities.Additionally, an enzymatic inhibition assay showed that Ctx and its …
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Biophysical and structural studies of novel F420-dependent oxidoreductases in Mycobacterium tuberculosis
… of F420 using STD NMR and differential scanning fluorimetry. Rv1155 and Rv2991 show affinity for distinct classes of fragments. Using a computational method known as “virtual fragment linking,” fragment binding data was used to predict and test potential substrates. This approach to probing …
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Large-scale studies and biophysical analysis of systems involved in plant immunity.
… a biophysical method, differential scanning fluorimetry (DSF). We tested the usefulness of these methods by interrogating different immune proteins/genes of the model plant Arabidopsis thaliana. Here is a summary of the major results obtained. In the realm of basal immunity, we discovered …
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Structure guided discovery of inhibitors of indoleamine 2,3-dioxygenase (IDO1)
… Work in this thesis used differential scanning fluorimetry and enzyme inhibition assays to screen a fragment library for novel IDO1 inhibitors. Thirty‐five small compounds that interacted with and inhibited the IDO1 enzyme were identified, and included compounds from the chemical classes: …
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Development of novel lysine targeting covalent inhibitors for Bruton’s tyrosine kinase and casein kinase 2 and synthesis of novel hybrid androgen receptor inhibitors
… mass spectrometry and differential scanning fluorimetry, whereby all analogues induce expected responses with the WT BTK, but fail to induce a response with the loss of function mutant R28C, as expected. A 6-fold improvement in binding affinity from the parent compound was achieved through a …
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One-Carbon Bridge Stapling for use in Peptide-Drug Conjugates
… linker with 7-hydroxycoumarin was analysed using fluorimetry and quencher studies, to confirm hydrogen peroxide dependent release. The same observation was determined for three cytotoxin ROS-cleavable conjugates. Finally, two PDCs were synthesised through a CuAAC reaction with cytotoxin …
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Salinity, radon, and radon precursors in the soils of Grand Forks County, North Dakota
… soil samples were analyzed for uranium by fluorimetry, equivalent uranium (eU) by gamma-ray spectrometry, and for soil salinity. </p><p>Equivalent uranium, equivalent thorium, potassium-40, and derivative maps were produced using aerial γ-ray data collected during the National Uranium …
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Development of peptide inhibitors as molecular therapeutics against tandem-repeat proteins
… plasmon resonance and differential scanning fluorimetry assays using recombinant Cdc20. Furthermore, D-box peptides were able to bind exogenous Cdc20 in mammalian cell lysates using a Cellular Thermal Shift Assay (CETSA). Lastly, I determined the co-crystal structures of three of the highest …
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