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Showing 1 to 3 of 3 for “"Fluoreszenz-Resonanz-Energie-Transfer"”.

  1. Konformationsänderungen im katalytischen Zyklus der RNA-Helikase YxiN - Fluoreszenz-Resonanz-Energie-Transfer in einzelnen Molekülen

    … RNA-Entwindung wurde in einem dafür entwickelten fluoreszenzbasierten Test in Polyacrylamidgelen nachgewiesen. Für Einzelmolekül-FRET-Experimente wurde YxiN mit zwei Fluorophoren markiert. Dazu mussten zunächst die zugänglichen Cysteine des Wildtyps durch Serin ersetzt werden. Anschließend wurden …

    bayreuth Repository record for Konformationsänderungen im katalytischen Zyklus der RNA-Helikase YxiN - Fluoreszenz-Resonanz-Energie-Transfer in einzelnen Molekülen (opens in a new tab)

  2. The ATP synthase from spinach chloroplasts (Spinacea oleracea) : improved isolation and purification of the enzyme and conformational changes observed with fluorescent labels

    … with the help of Fluorescence Resonance Energy Transfer (FRET), Fluorescence Lifetimes (FL) or with Fluorescence Correlation Spectroscopy (FCS). <br>Fluorescent probes were used to selectively label different subunits of CF1, in order to conduct spectroscopic investigations of conformational …

    freiburg-diss Repository record for The ATP synthase from spinach chloroplasts (Spinacea oleracea) : improved isolation and purification of the enzyme and conformational changes observed with fluorescent labels (opens in a new tab)

  3. Applications of two focus fluorescence correlation spectroscopy in colloid and polymer science

    Thermal fluctuations in liquid samples can be used to calculate the hydrodynamic radii (Rh) of observed particles. Therefore, it is necessary to generate scattering contrast between the observed particles and the surrounding liquid phase. In static- and dynamic light scattering (SLS/DLS) …

    aachen Repository record for Applications of two focus fluorescence correlation spectroscopy in colloid and polymer science (opens in a new tab)