Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 50 for “"Fluorescent in situ hybridization"”.
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Morphological classification of childhood medulloblastomas with β-catenin immunohistochemistry and mycn fluorescent in situ hybridization
Medulloblastoma is the most frequently occurring childhood malignant brain tumour, affecting 1 of 5 children presenting with a brain tumour, between the ages of 0 and 9 years. The basic prognostic stratification that relies on clinical and histological findings alone has proven unsatisfactory as an …
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Real time PCR and fluorescent in situ hybridization in the detection of the physical tsate of human papillomavirus 16 and 18 in paraffin embedded cervical tissue
… of most cervical dysplasia and all cervical carcinoma. Integration of high risk HPV into the human genome is thought to be a critical event in the progression from cervical dysplasia to invasive cervical carcinoma. The ability to use molecular assays in the detection and evaluation of HPV …
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Fast image and data processing methods for novel neuroscience technologies
… is a suitable model organism for whole-brain measurement of nervous activity. However, under panneuronal labeling, it is difficult to resolve the identity of the neurons by shape or location alone. We propose a fluorescent in situ hybridization (FISH) based pipeline for reading out gene …
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Chromosomal localization of a proinsulin transgene inserted with a transposon-based vector into Japanese quail, Coturnix coturnix
… were to develop a reproducible method of detecting stable DNA insertion into Japanese quail and provide a method for gene location on avian chromosomes. This research resulted in the development of a different method of obtaining chromosome spreads in Japanese quail, the establishment of primed …
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The promise and perils of molecular combing
<p>Dynamic Molecular Combing (DMC) is a method of making visible single molecules of DNA. The method stretches the molecules, attaching them to a glass coverslip; labels them with an intercalating dye or fluorescent antibodies; and images the molecules with fluorescent microscopy. After combing, …
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Development of Optical Tools and Techniques Toward a Functional Connectomic Understanding of C. elegans
… neural activity are popular and wellestablished in the field, but many of the most commonly used optical tools leave much to be improved upon. In this research I sought to establish the utility of new optical tools in C. elegans to address the shortcomings of commonly used ones. First, I present …
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Autosomal random asynchronous replication is analogous to X-chromosome inactivation
… genes are expressed from only one of two alleles in either an imprinted or random manner. Those belonging to the random class include X-linked genes subject to X inactivation, as well as a number of autosomal genes, including odorant receptors, immunoglobulins, T-cell receptors, interleukins, …
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Introducing the starlet sea anemone Nematostella vectensis as a model for investigating microbial mediation of health and disease in hexacorals
All animals in their natural state harbor complex communities of microbes including those that are beneficial (symbionts), neutral, or harmful (pathogens). The dynamic interactions between animals and their microbiota often dictate the balance between health and disease. Environmental perturbations …
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Anammox-based Technologies for Sustainable Mainstream Wastewater Treatment: Process Development, Microbial Ecology and Mathematical Modeling
… biological nitrogen removal, but its application in treating mainstream wastewater remains a great challenge. The key objectives of this dissertation are to develop nitritation-anammox process to treat wastewater with low-nitrogen strength, understand the fundamental microbiology, and optimize its …
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Optimization of Cytogenetic and Physical mapping of Culicinae genomes
Understanding chromosome structure and genome organization of Culicine mosquitoes can potentially contribute to the development of novel approaches to vector control. However, because of highly repetitive nature of the Aedes and Culex genomes, the structure of their polytene chromosomes is damaged …
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Spatial Relationship between the Trichloroethylene Degrading Bacteria Dehalococcoides Sp., Sulfate Reducers and Archaea during Reductive Dechlorination
… is of great concern due to its toxicity and carcinogencity. The microorganisms involved in bioremediation of TCE such as methanogens (organisms within the domain Archaea), sulfate reducing bacteria (SRB) and Dehalococcoides sp. (DHC) are of particular interest in this study. Three different types …
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Epigenetic Regulation of Skin Development and Postnatal Homeostasis The role of chromatin architectural protein Ctcf in the control of Keratinocyte Differentiation and Epidermal Barrier Formation
… regulatory mechanisms play important roles in the control of lineage-specific differentiation during development. However, mechanisms that regulate higher-order chromatin remodelling and transcription of keratinocyte-specific genes that are clustered in the genome into three distinct loci …
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Studying Tissue Specific Diversity of Splicing Regulator Muscleblind
<p>The muscleblind (mbl) family of RNA-binding proteins regulates alternative splicing, determining mRNA transcript composition for various types of tissue, and has been implicated in myotonic dystrophy. The mbl gene is subject to alternative splicing in Drosophila, leading to multiple isoforms, …
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Effects of Organic and Conventional Agricultural Practices on Soil Microbial Communities and Molecular Detection of Soil Borne Disease
… microbial communities and health through the input of pesticides, herbicides, fertilizers, and cycling of crop rotation. By examining the microbial community structure, we analyzed how microbial species respond to the environment that individual farms create. Early detection of soil borne …
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In situ genome sequencing: genomic measurement at the convergence of structure and molecular identity
… the function and behavior of molecules in the context of whole individual cells. Progress has been largely driven by two families of measurement technology: microscopy and DNA sequencing. Microscopy directly provides rich information on structure and organization, while sequencing …
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New insights on the ecology of free-living heterotrophic nanoflagellates based on the use of molecular biological approaches
… responsible for various trophic processes in marine and freshwater ecosystems. Current methods for identifying small protists do not readily permit identification and enumeration of nanoplanktonic flagellates in cultures or field samples. The aim of this study was to apply molecular …
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Über die Arc-catFISH-Methode als neues Werkzeug zur Charakterisierung der Geschmacksverarbeitung im Hirnstamm der Maus
Intensive Forschung hat in den vergangenen Jahrzehnten zu einer sehr detaillierten Charakterisierung des Geschmackssystems der Säugetiere geführt. Dennoch sind mit den bislang eingesetzten Methoden wichtige Fragestellungen unbeantwortet geblieben. Eine dieser Fragen gilt der Unterscheidung von …
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Behavioral and genetic characteristics of intestinal cell lineages in health and disease
The intestinal crypt is a highly dynamic system, as the entire epithelium is constantly turned over and renewed by the proliferative stem cells located at the bottom of the crypt. While this system is crucial for nutrient absorption, any derangements in the proliferative cells can quickly lead to …
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Identity and dynamics of the microbial community responsible for carbon monoxide oxidation in marine environments
As colored dissolved organic matter in seawater absorbs UV solar radiation, a variety of simple chemical species are produced, including carbon monoxide (CO). The ocean surface water is saturated with respect to CO, and is thus a source of CO to the atmosphere. CO reacts with and removes …
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