Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Fluorescent Resonance Energy Transfer (FRET)"”.
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Mechanotransduction of subcellular AMPK and its role in breast cancer cell migration
… and the potential link between cellular energy and cell migration remain poorly understood. In this study, we investigated the role of mechanotransduction (via Src and FAK) in AMP-activated protein kinase (AMPK) activation in breast cancer cells in response to interstitial fluid flow. …
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Rapid Rac GTP-ASE activation in live cells by mechanical stress is independent of Src
… guanosine triphosphatase, was quantified using a fluorescent resonance energy transfer (FRET) method that measures changes in Rac activity in response to mechanical stresses by quantifying intensity ratios of ECFP (enhanced cyan fluorescent protein as a donor) and YPet (a variant yellow …
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The design, synthesis and evaluation of selective, non invasive imaging agents for atherosclerotic plaque.
… plaques. This was achieved by using a Fluorescent Resonance Energy Transfer (FRET) active system to evaluate the substrate specificity of synthesized peptide sequences for legumain. The self quenched FRET substrates were engineered to carry a legumain sensitive bond in between the donor …
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Super-Resolution Force Spectroscopy for Revealing Sub-nucleotide Motion in Ribosomal Frameshifting and DNA Methylation
… cryo-electron microscopy (Cryo-EM) and fluorescent resonance energy transfer (FRET) have been utilized to study the translocation mechanism. However, it remains challenging to directly resolve the different reading frames. This dissertation focuses on applying super-resolution force …
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Role of an Adenylyl Cyclase Isoform in Alcohol's Effect on Cyclic AMP Regulated Gene Expression in Mammalian Cells
… To examine these objectives, time-lapse fluorescent resonance energy transfer (FRET) and cAMP accumulation assays were used to monitor cAMP levels within the cells. A reporter gene (luciferase) driven by an artificial promoter inducible with cAMP was utilized to evaluate the effect of …
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Macromolecular Organization and Cell Function: A Multi-System Analysis
… of flavonoid enzymes in living cells, using fluorescent lifetime imaging microscopy fluorescent resonance energy transfer analysis (FLIM-FRET), is presented in Chapter 4.
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Novel molecular engineering approaches for genotyping and DNA sequencing
… increase the read-length of SBS with cleavable fluorescent nucleotide reversible terminators (CF-NRTs) and nucleotide reversible terminators (NRTs) or hybrid-SBS with cleavable fluorescent nucleotide permanent terminators and NRTs. The idea of the walking strategy is to recover the initial …