Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"Fluorescent Imaging"”.
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DEVLOPEMNT OF FLUORESCENT IMAGING TOOLS FOR LIVE CELL IMAGING
… cellular processes and behaviors in real time. Fluorescent live cell imaging presents an appealing approach to learn about cell dynamics and function by providing specificity and high resolution. However, one of the biggest limitations of these imaging modalities is penetration depth. With …
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Studies of fluorescent imaging for mRNA detection in living cells
This dissertation focuses on the study of imaging mRNA in living cells. To achieve this research objective, three approaches have been utilized:: 1) Imaging of a transgenic mRNA tagged by multiple repeats of malachite green: MG) binding aptamer.: 2) Imaging of inducible nitric oxide synthase: iNOS) …
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An Efficient Architecture For Networking Event-Based Fluorescent Imaging Analysis Processes
… algorithms and tools utilized for analysis of fluorescent imaging data. Although an extensive collection of cloud-computing solutions exist allowing software developers to resolve these issues, such solutions often abstract both developers and integrators from the executing hardware particulars …
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Engineering fluorescent imaging platforms to characterise molecular interactions with HIV-1 capsid
The HIV-1 capsid is a protein shell utilised by the virus as a binding platform to hijack host machineries to promote infection. Characterisation of novel cofactors binding to capsid demands sensitive and quantitative assays to better understand their underlying binding mechanisms. Here, I have …
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Polymer Dynamic Organic Theranostic Spheres for Photothermal Therapy and Fluorescent Imaging of Cancer
Nanoparticle mediated photothermal ablation of cancer is a promising technique that utilizes light energy to destroy cancer cells. Specifically, nanoparticles that absorb in the near infrared (NIR) region of light, 700 - 900 nm, are optimal because these wavelengths are an absorption minimum for …
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Brain Signal Quantification and Functional Unit Analysis in Fluorescent Imaging Data by Unsupervised Learning
… animal models (zebrafish and mouse), and imaging modalities (two-photon, light sheet, and confocal), AQuA2 consistently delivers promising results and novel insights, showcasing its versatility in fluorescent imaging data analysis.
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Near-Infrared High Efficiency Organic Dye Sensitized Solar Cells (DSCs) and Biological Fluorescent Imaging Dyes
With the energy consumption increase every year, the non-renewable energy sources such as fossil fuels, natural gas, and coal will not sustain forever. In this case, searching for a way to develop a renewable energy source is an emergency. For decades, dye-sensitized solar cells (DSCs) have …
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Parallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator control
High resolution imaging in three dimension is important for biological research. The RESOLFT (Reversible Saturable Optical Fluorescence Transitions) fluorescent microscopy is one technique which can achieve lateral super-resolution imaging. Two-photon microscopy naturally generate high resolution …
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Anti-inflammatory drugs for modulation of host response to biomaterials and application in diabetes therapy
… Firstly, we developed a new non-invasive in vivo imaging technique to study the activity of early immune cells in the host response to implanted biomaterials. A fluorescent imaging probe (Prosense680*, Perkin Elmer) activatable by cathepsins, a class of inflammatory proteases secreted from immune …
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Development and Investigation of the Fluorescence of Cyclopropenium Ions
… design element towards the goal of developing fluorescent, superbasic and boronium-substituted compounds. A novel guanidine-cyclopropenimine proton sponge with exceptional basicity is reported that was further utilized to develop a stable tetracoordinate boronium-substituted proton sponge. A …
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Mixing with complex patterns : from the impact of miscible viscous fingering to the effects of motile bacteria
… flows in porous media, and high-resolution fluorescent imaging, to measure the temporal and spatial evolution of the mixing zone. We identify distinct regimes in both the interface length and average thickness of the mixing zone.
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Records of Great Basin precipitation during MIS 11 from two Lehman Cave stalagmites
… into two parts due to a hiatus observed through fluorescent imaging. The section prior to the hiatus spans 411 ka to 402 ka. The post-hiatus section of the stalagmite has a single age of 383 ka; an age model cannot be constructed for this part of the record. These stalagmites span Marine Isotope …
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Investigation of Akirin Nuclear Localization and the Molecular Interaction between Akirin and Taf4 in Drosophila
… calcium phosphate transfection of S2 cells and fluorescent imaging, we were able to compare wild-type full-length Akirin protein fused to the Venus fluorescent protein to full length Akirin::Venus fusion protein with alanine mutations in the Akirin NLS to determine if a change in nuclear …
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Manipulating macrophages to enhance liver regeneration
… of hepatic clearance of indocyanine green by fluorescent imaging and assessment of hepatic phagocytic capacity using fluorescent microbeads. I then describe macrophage based therapeutic interventions in mouse models of liver injury. Firstly the direct administration of bone marrow derived …
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Multimodal imaging monitoring of key physiological changes of peripheral arterial disease
… To define these landmarks, we use a multimodal imaging approach starting with Laser Speckle Contrast Imaging (LSCI) and Power Doppler ultrasound for perfusion (superficial and deep tissue, respectively); followed by fluorescent imaging of hypoxia and imaging both angiogenesis and metabolism …
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Wide-field structured illumination microscopy for fluorescence and pump-probe imaging
… limited resolution, but mostly focused on fluorescent imaging. Furthermore, there are few non-fluorescence wide-field super-resolution techniques that can fully utilize the applicable laser power to optimize imaging speed. Structured illumination microscopy is a super-resolution method that …
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Near-infrared emitting quantum dots for cellular and vascular fluorescent labeling in in vivo multiplexed imaging studies
… been limited by the paucity of suitable probes fluorescent in the near-infrared spectrum that is favored by tissue optics. This project attempts to address this problem by developing cellular and vascular fluorescent imaging probes comprised of semiconductor nanocrystals, or quantum dots (QDs), …
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The detection of nitric oxide and its reactivity with transition metal thiolate complexes
… To this end, we seek to create tools for the fluorescent imaging of NO in vivo. We have adapted an existing small molecule-based sensor for more biologically relevant applications by including it within a polymeric film. We have also developed turn-on fluorescent sensors for NO based on …
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Microfluidics and live imaging advances: applications in host/pathogen, immunity and stem cell single cell phenotyping
Live single-cell imaging has emerged as an advanced single-cell study tool for approaching a quantitative understanding of many biological questions in recent years. In previous cell studies using bulk cell measurements, the population averages can miss the information from cell to cell variability …
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Real-time Image Processing on an FPGA for an Intraoperative Goggle Device
… emergence of near-infrared dyes for fluorescence imaging has had a tremendous impact in the medical field. In particular, indocyanin green (ICG) has been widely used for assessing tumor margins during intraoperative procedures. Typically, the dye is intravenously injected into the patient, and …
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