Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Fluorescent Assay"”.
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Confocal laser scanning microscopy of nanoparticles applied to immunosorbent assays
… and develop a confocal readout method for fluorescent immunosorbent assays and investigate its potential advantages in comparison to traditional immunoassays. The key point of a confocal immunosorbent assay is the ability to detect the thin layer of immunoassay in the presence of unbound …
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The role of proteases in development of cancer in mice
… liver and skelatal muscle. Using a modified fluorescent assay, differences in m-calpain and calpastatin activity were found between seven and nine month ethyl carbamate treated mice. The calpain to calpastatin ratio is higher in the nine month treated mice due to a decline in the activity of …
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Directed evolution of a subtilisin Carlsberg variant towards improved perhydrolytic activity for industrial application
… system. To overcome this obstacle, a sensitive, fluorescent assay with detection limit below 1 µM of peroxyacetic acid and suitable for detection of perhydrolytic activity without previous enzyme purification was developed. The assay was optimized for screening in 96 and 384-well microtiter plate …
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Vitamin B12 Bioconjugates In Pharmaceutical Design
… small intestinal cells?</p> <p>Goal: Design a fluorescent assay to establish functional cubilin and megalin expression and conduct western blotting to complement observations.</p> <p>Q4: (Chapter 4) Does B12-Exendin-4, a conjugate of B12 and the diabetes drug exendin-4 (Ex-4; a peptide agonist …
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The analysis of a conserved RNA structure in the 3D polymerase encoding region of Human Parechovirus 1
… structure has a role in RNA stability, an EGFP fluorescent assay was used. Sequences containing the structure was added to the 3’ UTR of the EGFP gene. A mutant with 21 mutations which completely destroys the RNA structure was also used. A FACS-based method was used to measure expression levels …
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Chemical controls on the cycling and reactivity of marine dissolved organic matter
… the lower euphotic zone using a newly developed fluorescent assay, which measures the activity of carbon-phosphorus lyase. C-P lyase activity (CLA) profiles from the North Pacific Subtropical Gyre (NPSG) showed a sharp activity maximum near the deep-chlorophyll maximum (DCM). High-resolution …
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Chemical Controls on the Cycling and Reactivity of Marine Dissolved Organic Matter
… the lower euphotic zone using a newly developed fluorescent assay, which measures the activity of carbon-phosphorus lyase. C-P lyase activity (CLA) profiles from the North Pacific Subtropical Gyre (NPSG) showed a sharp activity maximum near the deep-chlorophyll maximum (DCM). High-resolution …
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CRISPR-based approaches for the detection of antimicrobial-resistant genes
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01
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Methods of cellular integration and techniques for improving encapsulated cell viability for the purpose of developing living materials and cell based bio-sensing materials
… Findings based on a superoxide indicating fluorescent assay suggest that inclusion of manganese phosphate buffer reduces oxidative stress immediately upon integration and that oxidative stress is likely a primary stress in the first few hours of integration. Addition of fluid (DOPC) or …
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Immunotoxic Effects of Mixtures of Endosulfan and Permethrin Via Caspase Dependent Thymocyte Apoptosis
… and late apoptotic/necrotic cells. DNA ladder assay, a hallmark of apoptosis, was also used to determine the occurrence of apoptosis. Both endosulfan and permethrin were found to cause significant apoptotic death of thymocytes in a dose- and time- dependent manner. Thus, permethrin at 50, 100 …