Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Fluorescence signals"”.
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Detection and interpretation of fluorescence signals generated by excitable cells and tissues
… 1: High-Sensitivity Amplifiers for Detecting Fluorescence<br />Monitoring electrical activity and Ca<em>i</em> 2+ transients in biological tissues and individual cells increasingly utilizes optical sensors based on voltage-dependent and Ca<em>i</em> 2+-dependent fluorescent dyes. However, …
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Nano-structured platforms as a spectroscopic tool
… been studied for the purpose of enhancing weak fluorescence signals. Each platform was constructed as a thin oxide layer on top of a metal (aluminum) The oxide was perforated with hexagonal array of nano-holes. The pitch of the array was much smaller than the optical wavelengths used. Surface …
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Alternative strategies for security labelling/encoding of paper and plastic products.
… detect all three dopants based on their discrete fluorescence signals and unique fluorescence lifetimes has been achieved with an in-situ detector. This has advantages over current fluorescent dye labels which have broadband spectra that quickly overlap each other reducing their number of …
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Properties of Nanoscale Biomaterials for Cancer Detection and Other Applications
… and blood sera. The resulting Raman signals from sera collected from horses with malignant melanoma were found to have additional peaks not found in the Raman signals obtained from sera collected from healthy horses. A systematic analysis of the combination of absorbance and …
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Signal Processing Techniques Applied to Biomedical Diagnostics
… to boost the signal-to-noise ratio of optical fluorescence signals. The LIA is designed to be resilient in challenging environments containing high/unpredictable ambient noise. We then develop two rapid diagnostic systems that pair this technology with isothermal CRISPR-Cas12a-based DNA/RNA …
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Fluorescence lifetime-resolved imaging microscopy studies: quantitative image analysis, spectral-FLIM, and photosynthesis
… and image analysis methods for the fluorescence lifetime-resolved imaging microscopy (FLIM) were carried out in this thesis. With the new instrument setup and imaging processing algorithms, valuable information has been provided for studying in vivo photosynthesis activities. Chapter …
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Photophysical characterization of toluene and 3 pentanone for quantitative imaging of fuel/air ratio and temperature in combustion systems
… for fuel visualization based on laser-induced fluorescence (LIF) has grown to an important engineering tool in modern engine research. However, quantitative interpretation of fluorescence signals in terms of fuel/air ratio or temperature requires sound fundamental knowledge of the compound’s …
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Effect of hypoxia on spontaneous neural activity in the cortex of neonate mouse pups
… – 40 weeks). This study utilized wide-field epifluorescence imaging to indirectly record neural activity in the form of fluorescence signals arising from a large volume of brain (from lambda suture to bregma) under normal air and hypoxic air in SNAP-25-2A-GCaMP-6s transgenic mice at postnatal …
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Effect of hypoxia on spontaneous neural activity in the cortex of neonate mouse pups
… – 40 weeks). This study utilized wide-field epifluorescence imaging to indirectly record neural activity in the form of fluorescence signals arising from a large volume of brain (from lambda suture to bregma) under normal air and hypoxic air in SNAP-25-2A-GCaMP-6s transgenic mice at postnatal …
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Remote three-dimensional temperature sensing using planar laser induced fluorescence : development and applications to microwave heated liquids
… thesis describes the development of a 3D imaging fluorescence thermometry system that was used to examine temporal and spatial variations in temperature within various aqueous solutions during their heating by microwave irradiation. The work provides one of the first experimentally-determined …
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Sensitive Detection of Foodborne E. coli O157:H7 by Dendrimer-aptamer Modified Microchannels - with RCA Signal Intensifications
… sensitivity, RCA is employed to intensify fluorescence signals for detection. To better understand the RCA process and optimize its reaction conditions, the RCA reaction processes are followed over time using atomic force microscopy and agarose gel electrophoresis. Subsequently, the …
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Spectroscopic Technologies and Computational Approaches for Real-Time Detection of FMN as Biomarker during Machine Perfusion
… measurements, UV-visible absorption, and fluorescence, using both standard laboratory instruments, such as benchtop spectrophotometers, and an innovative stand-alone device, specifically developed by the company funding the doctoral research, capable of detecting fluorescence signals …
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Optimization of Optical Nanosensor Response for the Detection of Anthracyclines Using a Binary Machine Learning Classifier
… development, as they exhibit near-infrared fluorescence in the tissue-transparent window and a robust response to their local environment. An emerging method for evaluating and optimizing SWCNT sensor response is through machine learning. In this study, anthracyclines Daunorubicin, …
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Catalytic Studies Of Noble Metal Nanocatalysts Using Single-Molecule Fluorescence Microscopy
… to quantify these differences. A highthroughput fluorescence microscopy technique was developed in which a nonfluorescent reactant gets catalyzed to a fluorescent product on a gold catalyst surface. By recording the fluorescence signals at the single-molecule level from many particles on a camera …
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Highly sensitive biosensors using photonic crystal enhanced fluorescence
Due to the importance of fluorescence assays in clinics, life science and genomic research, many nanostructures have been proposed to enhance the weak fluorescence signals from low abundance analytes. Among these structures, photonic crystal (PC) has attracted particular attentions because of its …
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The development of steady State and lifetime fluorescence instruments for real time in situ aquatic chemistry measurements
… a spectrometer to measure steady state chemical fluorescence and absorbance. The instrument is packaged aboard an autonomous underwater vehicle (AUV) and demonstrates the ability to map chemical concentrations in three dimensions. The second instrument repackages the sensor components to study …
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Vector magnetometry using cavity-enhanced microwave readout of solid-state spin sensors
… techniques that go beyond measuring optical fluorescence signals, which are often difficult to detect, and unique only to some solid-state spin systems. In this thesis, I discuss these two approaches, and begin by designing a three dimensional microwave resonator that overcomes the many …
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Developing Tools to Physically Magnify Biological Substrates for Clinical Applications
… aggressiveness. dExPath results in improved fluorescence signals even as it eliminates lipofuscin-associated autofluorescence. Thus, this form of expansionmediated protein decrowding may, through improved epitope access for antibodies, render immunohistochemistry more powerful in clinical …
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The development of methods for the determination of selenium using spectrofluorimetric and ICP-MS techniques.
… type and concentration of acid. The most intense fluorescence signals were observed in HNO3 and at a PTQA concentration of 5x10[-4] M. A sample throughput of 8 samples/hr was achieved. Method validation was by comparing the results obtained for the determination of selenium in nutritional …
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Microfluidic-based Fluorescence Detection for Biomarker Analysis
… with the combination of microfluidics and fluorescence detection for biomarker analysis. The first platform described here is to isolate CTCs with high purity by using 2-stage sorting technique and herringbone features for aliquot stretching, including axial and lateral mixing to spread …
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